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Biosensor

A technology of biosensors and substances, applied in the field of biosensors, can solve problems such as inability to completely eliminate errors in measurement results

Inactive Publication Date: 2005-02-02
PANASONIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But even with these techniques, errors in the measurement results cannot be completely eliminated

Method used

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Embodiment 1

[0050] As an example of a biosensor, a figure 1 and figure 2 The shown structure of the glucose sensor. figure 1 It is an exploded perspective view of the glucose sensor with the reagent layer and the like removed. figure 2 yes figure 1 Cross-sectional view of the X-X line.

[0051] On an electrically insulating substrate 1 made of polyethylene terephthalate, silver paste was printed by screen printing to form leads 2 and 3 . Next, a conductive carbon paste containing a resin binder is printed on the substrate 1 to form the working electrode 4 . The working electrode 4 is brought into contact with the lead wire 2 .

[0052] Next, an insulating paste is printed on the substrate 1 to form the insulating layer 5 . Covering the outer peripheral portion of the working electrode 4 with the insulating layer 5 ensures that the exposed portion of the working electrode 4 has a constant area. Also, parts of the leads 2 and 3 are covered with an insulating layer 5 . Then, a cond...

Embodiment 2

[0063] In this embodiment, a substrate 1 and a cover member 8 made of glass are used. Biosensors were fabricated in the same manner as in Example 1. However, the formation of electrodes and leads by printing insulating paste has not been performed.

[0064] Drop carboxymethylcellulose (CMC) aqueous solution on the substrate 1, make it dry to form a CMC layer, and then on the CMC layer, as an enzyme, drop GOD as an enzyme and 1-methoxy- An aqueous solution of 5-methylphenazinium (1-Metokishi-5-Mecholphenazinium) was dried to form a reagent layer.

[0065] Next, in order to supply the sample solution onto the reagent layer more smoothly, the toluene solution of lecithin is diffused from the sample solution supply port to the reagent layer and dried to form a lecithin layer on the reagent layer.

[0066] Then, an interfering substance processing member is provided on the surface of the part divided by sticking the spacer member 7 on the cover member 8 . An appropriate amount o...

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Abstract

The present invention is to provide a biosensor capable of determining the concentration of a substrate with higher precision. The biosensor comprises an electrically insulating substrate, a measuring system, a reagent layer including at least an oxidoreductase and an electron mediator, and the biosensor has a member for elimination of interfering compounds including: a redox agent which functions as an oxidant for oxidizing interfering compounds in a sample, and a carrier for immobilizing the redox agent.

Description

technical field [0001] The present invention relates to a biosensor capable of quickly and accurately quantifying a matrix in a sample with ease. Background technique [0002] Various types of biosensors utilizing the special catalytic action of enzymes have been developed in recent years as quantitative analysis methods for sugars such as sucrose and glucose. Here, as an example of a method for quantifying a substrate in a sample solution, a method for quantifying glucose will be described. As an electrochemical glucose quantification method, a quantitative method using glucose oxidase (EC 1.1.3.4, hereinafter abbreviated as GOD) and an oxygen electrode or a hydrogen peroxide electrode is known. [0003] GOD uses oxygen as the electron carrier to selectively oxidize the substrate β-D-glucose to form D-glucono δ-lactone. In the presence of oxygen, oxygen is reduced to hydrogen peroxide during the oxidation reaction caused by GOD. The decrease in oxygen is measured by an o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/00G01N33/487
CPCC12Q1/004G01N27/3274
Inventor 池田信中南贵裕吉冈俊彦桑畑进
Owner PANASONIC CORP
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