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Gel capable of injecting temperature sensitive complex and its preparation method

A complex and gel technology, applied in the field of material science, can solve problems such as unfavorable salt resistance, and achieve the effect of broadening the scope of application and maintaining excellent performance.

Inactive Publication Date: 2005-03-16
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to make this chitosan gel solution gel at body temperature, the concentration of the salt used must reach more than 4.0% w / w, if it can be gelled at lower temperatures, it will be more effective at body temperature. With short gel time and greater gel strength, it is necessary to further increase the salt content of the system, which is unfavorable for encapsulating some drugs and cells with poor salt tolerance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1. prior art:

[0027] Accurately weigh 1.0 g of chitosan and add it to 45 ml of 0.1 mol / l HCl solution. After dissolving at room temperature for 2 hours, filter with a G3 glass sand core funnel to filter out insoluble substances, and take 9 ml of chitosan filtrate. Weigh 420 mg of β-GP and dissolve it in 1 ml of distilled water, and carefully add it dropwise into the chitosan filtrate under continuous stirring to obtain a uniform and clear solution, and then continue to stir for about 20 min. The pH of the final solution was 7.0.

[0028] The prepared complex solution is in a liquid state at room temperature, and gels at a body temperature of 37°C. The gelation time at 37°C is about 10 minutes, and the gel strength is about 0.126KPa.

Embodiment 2

[0030] Accurately weigh 1.0 g of chitosan and add it to 45 ml of 0.1 mol / L HCl solution. After dissolving at room temperature for 2 hours, filter with a G3 glass sand core funnel to filter out insoluble substances, and take 9 ml of chitosan filtrate. The GP (the percentage content of α-GP in GP is 30%w / w) that takes by weighing 420mg is dissolved in 1ml distilled water, under the condition of continuous stirring, carefully drop in the chitosan filtrate, finally obtain uniform and clear solution, and continue to stir for about 20 min. The pH of the final solution was 7.15.

[0031] This complex solution, compared with the system using β-GP alone in Example 1, without increasing the salt content of the system, IGT decreased from 37°C to 29°C, and the gelation time at 37°C was shortened from 10min to 200s, and the gel strength increased about 4 times.

Embodiment 3

[0033] Accurately weigh 1.0 g of chitosan and add it to 45 ml of 0.1 mol / L HCl solution. After dissolving at room temperature for 2 hours, filter with a G3 glass sand core funnel to filter out insoluble substances, and take 9 ml of chitosan filtrate. Weigh 50 mg of disodium hydrogen phosphate and 200 mg of β-GP and dissolve them in 1.0 ml of distilled water, and carefully drop them into the chitosan filtrate under continuous stirring to obtain a uniform and clear solution, and then continue to stir for about 20 min. The pH of the final solution was 6.95.

[0034] The complex solution can gel at 37°C, and the gelation time and gel strength at 37°C are equivalent to those of the system using β-GP alone in Example 1, but the salt content in the system is changed from 4.0%w / The w dropped to 2.4% w / w, a 40% drop in salt content.

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PUM

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Abstract

The invention provides a gel capable of injecting temperature sensitive complex and its preparation method, wherein the constituent percentage by weight of the composition includes chitosan 1.0-2.0, glycerin phosphate 1.9-4.5, disodium hydrogen phosphate 0-0.5, gelatin 0-1.25, 0.1mol / l HCL solution or acetic acid solution 86.4-89.0, distilled water 8.1-10. The gel can be used for encapsulating medicaments and cells with weak salt resistance.

Description

technical field [0001] The invention belongs to the technical field of material science, and in particular relates to an injectable temperature-sensitive complex gel and a preparation method thereof. Background technique [0002] Injectable gel scaffold material is a kind of biological material, which is liquid at room temperature and can wrap active cells and therapeutic drugs. A phase transition occurs at the injection site to form a gel scaffold. Injectable gel scaffolds have the advantages of easy operation, convenient shaping, reduced surgical difficulty, and reduced surgical trauma. They can be used in drug delivery, tissue repair, and cell culture. They have high medical value and broad application prospects. [0003] Chitosan is a copolymer of glucosamine and acetylglucosamine, which can be prepared by deacetylation of chitin under alkaline conditions. It is a natural cationic polysaccharide with good biocompatibility and biodegradabi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00
Inventor 成国祥吴燕
Owner TIANJIN UNIV
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