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Microorganism combined bacteria for straw fermentation and its process for producing protein fodder

A protein feed and production method technology, applied in the methods of using microorganisms, biochemical equipment and methods, microorganisms, etc., can solve the problems of low nutrient content of straw and difficult rapid transformation of microorganisms, etc., to improve the effective utilization and production methods. Scientifically reasonable and easy-to-promote effect

Inactive Publication Date: 2005-03-23
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pure physical and chemical methods did not solve the fundamental problem of low nutrient content in straw
The biological method has the characteristics of less pollution, high efficiency, and is conducive to industrial production. However, straw, a natural fibrous material, has a complex three-dimensional structure, especially the barrier effect of lignin, which makes it difficult for microorganisms to quickly transform it.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] In Example 1, 100 kilograms of dry corn stalk powder crushed to 80 meshes and 58 kilograms of water were mixed and put into an ammoniating pit for compaction and sealing, 20 kilograms of ammonia were injected from the bottom of the pit, ammoniated at 40°C for 12 days, and then taken out and dried. After drying, 3 kg of urea, 3.4 kg of inorganic salts, 20 kg of combined strains, and 425 kg of water were added into the fermentation chamber, and aerobic fermentation at 25°C for 5 days, and finished protein feed was obtained after drying. According to GB / T 6432-94, the crude protein content is 34.8%; take 1g (dry weight) of the finished product and add 30ml of 75% ethanol to extract for 1h, stir thoroughly, centrifuge at 4000rpm for 10min, discard the supernatant, and settle in 60 After drying at ℃ to constant weight, the true protein content is 29.4% according to GB / T 6432-94.

Embodiment 2

[0016] In Example 2, 100 kilograms of dry corn stalk powder crushed to 70 meshes and 65 kilograms of water were mixed and put into an ammoniating pit for compaction and sealing, 16 kilograms of ammonia were injected from the bottom of the pit, ammoniated at 35°C for 15 days, and then taken out and dried. After drying, 4 kg of urea, 1.7 kg of inorganic salts, 5 kg of combined strains, and 450 kg of water were added to the fermentation chamber, and aerobic fermentation at 28°C for 6 days. After drying, the finished protein feed was obtained. The protein content was measured according to the method of Example 1. The true protein content was 25.2%, and the crude protein content was 30.4%.

Embodiment 3

[0017] In Example 3, 100 kilograms of dry corn stalk powder crushed to 60 meshes and 60 kilograms of water were mixed and put into an ammoniating pit for compaction and sealing, 18 kilograms of ammonia water was injected from the bottom of the pit, ammoniated at 45°C for 9 days, and then taken out and dried. After drying, add 3.5 kg of urea, 2.3 kg of inorganic salts, 10 kg of combined strains, and 400 kg of water mixture into the fermentation chamber, aerobic fermentation at 30°C for 4 days, and the finished protein feed after drying. The protein content was measured according to the method of Example 1. The true protein content was 25.8%, and the crude protein content was 31.8%.

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PUM

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Abstract

The invention is a microbial combined bacterium used in stalk fermentation and a method of using it for producing protein feed. Its characteristic: the combined bacterium is composed of cellulose-decomposing Penicillium sp. and Torula allii; and its fermenting process: using pure stalks as raw material, firstly ammonifying the crushed stalks by ammonia water to enrich them with volatile carbon and nitrogen sources, then fermenting them by the combined bacteria, drying and then obtaining the finished product protein feed, where the fine protein content reaches 25-30% and the crude protein content reaches 30-35%, namely a high-grade protein feed. The producing method has simple process, easy to operate, low-cost and easy to spread.

Description

Technical field [0001] The invention relates to a method for producing protein feed by using microbial fermentation, in particular to a microbial combination bacteria used for straw fermentation and a method for producing protein feed by using the same. Background technique [0002] A large number of crop straws are a strategic reserve renewable resource. According to the United Nations Environment Programme (UNEP), various grains grown in the world can provide 1.7×10 straws per year. 9 t, my country’s various crop straw resources are very rich, with an annual output of up to 7.0×10 8 t. However, the utilization rate of straw material in my country is very low. In most areas, a large amount of straw is treated by incineration. The billowing smoke emitted emits a lot of harmful gases, which not only pollutes the atmospheric environment, affects climate change and ecological changes, but also It is also a serious waste of renewable resources, so exploring the effective use of straw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/32C12N1/00C12N1/14C12N1/22
Inventor 李日强
Owner SHANXI UNIV
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