Method for detecting phosphate radical in urine

A phosphate radical and urine technology, which is applied in the measurement of color/spectral properties and the analysis by chemical reaction of the material, can solve the problems of cumbersome, low sensitivity, unable to give accurate quantitative results, etc., and achieve a simple detection process. , large linear range, good selectivity

Inactive Publication Date: 2005-03-23
SHANXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This is both loaded down with trivial details, and time-consuming, brings a lot of inconvenience clinically
[0010] 3. Many me

Method used

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  • Method for detecting phosphate radical in urine
  • Method for detecting phosphate radical in urine
  • Method for detecting phosphate radical in urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1. prepare the HEPES (10mM) buffer solution of pH7.0, and prepare 2 * 10 -3 MYbCl 3 solution and 2 x 10 -3 Catechol violet solution of M; add 2ml of HEPES buffer solution to a clean UV cuvette as a blank, draw 2×10 -3 50 μl of catechol violet solution of M was added to the cuvette, at this time the solution turned from colorless to yellow, detected on the HP8453 UV-Vis spectrophotometer, and there was a maximum absorption at 443nm; in the above-mentioned cuvette, join 2×10 -3 M's Yb 3+ Solution 100 μl, at this time the solution changed from yellow to blue, detected on the UV-Vis spectrophotometer, and found that the maximum absorption peak changed from the above-mentioned 443nm to 623nm; take adult urine samples, and gradually add them to the cuvette with a micro-sampler , testing on the UV-Vis spectrophotometer while adding the sample, with the addition of the urine sample, the maximum absorption peak gradually changes from 623nm to 443nm, and the color ...

Embodiment 2

[0024] Embodiment 2. prepare the HEPES (10mM) buffer solution of pH6.5, all the other are with embodiment 1, and the phosphate radical content is 0.095% in the urine, and the ultraviolet-visible absorption figure is shown in figure 2 .

Embodiment 3

[0025] Embodiment 3. Get the healthy child urine of one year old, all the other are with embodiment 1, calculate: 100 μ l * 2ml * 95 / 4g * 100%, the phosphate radical content is 0.38% in the urine, and the ultraviolet-visible absorption figure sees image 3 . From the above examples, we can see that the content of phosphate in children's urine is relatively high. This is because compared with adults, children have higher alkaline phosphatase activity and similar metabolic rate due to the fact that children are in the period of vigorous osteogenesis. Wait for the reason.

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Abstract

The invention relates to a method for using ytterbium ion and catechol violet as developer to detect the content of phosphate radical in urine without being diluted in the HEPES buffer sotution with pH value 7.0 directly, qualitatively and quantitatively. The process is simple, convenient, and the result is accurate, the method has a high sensitivity, and it is not disturbed by the other elements in urine, and it also can be applied to clinic.

Description

Technical field: [0001] The invention relates to a method for detecting phosphate radicals, in particular to a method for detecting phosphate radicals in urine. Background technique: [0002] Phosphate is involved in various important biomineralization processes, such as bone formation and pathological processes such as the origin of kidney stones. Therefore, the detection of phosphate in biological fluids is important. We know that urine has a very important diagnostic value in clinical events, so it is necessary to detect free phosphate ions in human urine. Scientific research results show that excessive magnesium ammonium phosphate, carbonate apatite, hydroxyapatite, calcium hydrogen phosphate dihydrate, tricalcium phosphate and other crystals in urine are one of the causes of urinary stones. We know that stone disease is accompanied by many pains such as renal colic, nausea and vomiting, hematuria, fever, etc., and even causes other diseases. Therefore, it is critical...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/75
Inventor 杨频阴彩霞霍方俊
Owner SHANXI UNIV
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