Lichen bacillus strain (B-0A12) and preparation thereof

A technology of Bacillus licheniformis and bacterial agent, applied in the field of microorganisms, can solve the problems of prolonged and short liquid-solid two-phase fermentation time, only 3-6 months, and long fermentation period, so as to improve the rhizosphere micro-ecological environment and promote The effect of soybean growth and short fermentation period

Active Publication Date: 2005-06-01
北京启高生物科技有限公司
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Problems solved by technology

[0004] There are many domestic and foreign research reports on the application of nematode fungi and parasitic bacteria to control plant parasitic nematodes (Lin Maosong, 1990; Liu Xingzhong et al., 1991, 1992), and the fungi Pochonia chlamydosporum and Paecilomyceslilacinus for preventing and controlling soybean cyst nematodes have entered field experiments (Liu Xingzhong, Patent No.: ZL 951 21114.5; Pan Cangsang, Patent No.: CN1149393, CN1149394; Liu Weizhi, Patent No.: CN1225236), but due to the characteristics of the fungus itself, its application is limited. The fermentation time usually takes 5-7 days. If liquid-solid two-phase fermentation is

Method used

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Embodiment Construction

[0032] Bacillus licheniformis CGMCC No.1035 cultivated on the slant of beef extract peptone medium was used as the seed slant, and then cultivated according to the three-stage fermentation process of shake flask→seed tank→fermentation tank, the inoculation amount was 2%, and the medium formula used for:

[0033] Medium Components Content

[0034] Soluble starch 1% (W / V, the same below)

[0035] Bean cake powder 1%

[0036] Beef Extract 0.5%

[0037] Glucose 0.3%

[0038] NaNO 3 0.01%

[0039] Mg 2 SO 4 ·7H 2 O 0.02%

[0040] CaCO 3 0.2%

[0041] pH 7.0-7.2

[0042] Control the fermentation temperature at 28-30°C, pH7.0-7.4, set the secondary fermentation time at 20-24h, and set the tertiary fermentation time as the number of bacteria in the fermentation liquid reaches 7.0×10 8 per ml, the whole fermentation time is 60-72h. Add 0.5% (V / V) trace element mother liquor (ZnSO 4 1% (W / V), H 3 BO 4 0.1% (W / V), NaMoO 4 .2H 2 O 0.05% (W / ...

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Abstract

One strain for preventing and controlling root rot caused by soybean cytocyst nematosis and fusarium is named as Bucillus licheniformis CGMCC No. 1035. The liquid bacteria preparation for preventing and controlling root rot caused by soybean cytocyst nematosis and fusarium is prepared through inoculation with the said strain, fermentation and addition of additive. The fermentation process has temperature of 27-35 deg.c and pH 6.8-8.0; and the bacteria content in finishing fermentation is 700 million/ml. Corresponding solid preparation and seed coating preparation may be also prepared. The preparation for preventing and controlling soybean cytocyst nematosis and fusarium can also regulate microbial ecologic system around root system, promote plant growth and increase yield.

Description

technical field [0001] The invention relates to the field of microorganisms, more specifically, relates to a bacillus licheniformis bacterial strain and a preparation thereof for preventing and treating plant root diseases, in particular for preventing and treating soybean cyst nematode and root rot. technical background [0002] Soybean originated in my country. With the expansion of planting area, the problem of repeated cropping of soybeans has become more and more serious. Among them, the incidence area of ​​soybean cyst nematode and root rot accounted for more than 70% of the soybean planting area, and the loss reached 10-40%. The emergence of serious plots was irregular, the plants were short and yellow, commonly known as "fire dragon seedlings", and even Lead to extinct production, and the incidence area has an increasing trend in recent years. [0003] At present, the effective chemical drugs against soybean cyst nematodes and root rot are still using carbofuran and...

Claims

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Application Information

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IPC IPC(8): A01N63/00C12N1/20
CPCY02A40/10
Inventor 郭荣君李世东刘杏忠孙漫红缪作清张拥华杨怀文
Owner 北京启高生物科技有限公司
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