Nanometer oleanolic acid powder for injection and preparation method thereof
A technology for oleanolic acid and injection, which is applied in the field of nano-oleanolic acid powder injection for injection and its preparation field, can solve the problems of slow dissolution rate, low bioavailability, can not be improved and outstanding, etc. The effect of reducing side effects, simple preparation method and easy control of process route
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Embodiment 1
[0015] The process route shown in the accompanying drawing: Dissolve 0.25g of oleanolic acid and 8g of soybean lecithin with 150ml of acetone or chloroform or ethanol or methanol and filter to obtain solution A; Solution B, and heated to 55-65°C on a water bath; under stirring conditions, add oleanolic acid in acetone or chloroform or ethanol or methanol solution A, keep warm on a water bath for 2 hours; evaporate acetone or Chloroform or ethanol or methanol to obtain solution C; dissolve 300g of mannitol and 300g of glucose in 500ml of distilled water, mix and dissolve with solution C, set the volume to 5000ml, and divide into vials, 5ml per bottle, filled with neat Fruit acid 2.5 mg, freeze-dried to obtain the finished product.
Embodiment 2
[0017] The process route shown in the accompanying drawing: Dissolve 0.5g of oleanolic acid and 10g of soy lecithin with 200ml of acetone or chloroform or ethanol or methanol and filter to obtain solution A; dissolve 10g-15g of poloxamer with 4000ml of distilled water, and filter Solution B, and heated to 55-65°C on a water bath; under stirring conditions, add oleanolic acid in acetone or chloroform or ethanol or methanol solution A, keep warm on a water bath for 2 hours; evaporate acetone or Chloroform or ethanol or methanol to obtain solution C; dissolve 350g of mannitol and 300g of glucose in 500ml of distilled water, mix and dissolve with solution C, set the volume to 5000ml, and divide into vials, 5ml per bottle, filled with neat 5 mg of fruit acid was freeze-dried to obtain the finished product. (with or without hyperosmotic treatment)
Embodiment 3
[0019] The process route shown in the attached figure: Dissolve 1 g of oleanolic acid and 15 g of soybean lecithin with 150 ml of acetone or chloroform or ethanol or methanol and filter to obtain solution A; dissolve 15 g to 20 g of poloxamer with 4000 ml of distilled water and filter to obtain solution B, and heated to 55-65°C on a water bath; under stirring conditions, add oleanolic acid in acetone or chloroform or ethanol or methanol solution A, keep warm on a water bath for 2 hours; distill off acetone or tristimulus under reduced pressure Chloromethane or ethanol or methanol to obtain solution C; dissolve 450g of mannitol and 300g of glucose in 500ml of distilled water, mix and dissolve with solution C, set the volume to 5000ml, and divide into vials, 5ml per bottle, containing olean fruit acid 10mg, freeze-dried to obtain the finished product.
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