Preparation of triazoline artificial semi-antigen, antigen and antibody
A technology of artificial hapten and triazophos, which is used in the preparation of antibodies and the field of antigens, can solve the problems of cumbersome, complicated and toxic processes, and achieve the effects of high sensitivity, good affinity and low cross-reaction rate.
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[0036] Example 1
[0037] 1. The synthetic method of triazophos artificial hapten is as follows:
[0038] 1) Synthesis of O-ethylthiophosphoryl dichloride (TZLM-1)
[0039] Weigh phosphorus trichloride (PSCl 3 ) 68g (approximately 0.4mol) is placed in a three-necked flask with a low temperature thermometer, cooled to -10~-5℃ in an ice salt water bath, and 18.4g (approximately 0.4mol) of absolute ethanol is added dropwise under vigorous stirring. Strictly control the dropping rate so that the temperature of the reaction liquid is always -30~0℃. After the addition, the reaction was continued at -20~10°C for 1 hour. After the reaction, wash the reaction solution (100ml×2) with distilled water at (0±5)℃, separate the oil layer and use anhydrous Na 2 SO 4 After drying, distillation under reduced pressure by a water pump, and collecting 65-75°C fractions to obtain 28.5 g of colorless and transparent oily liquid (yield 40%, calculated as phosphorous trichloride).
[0040] 2) Synthesis of...
Example Embodiment
[0073] Example 2
[0074] 1. The synthetic method of triazophos artificial hapten is as follows:
[0075] 1) Synthesis of O-ethylthiophosphoryl dichloride (TZLM-1)
[0076] Weigh Phosphorus Trichloride (PSCl 3 ) 68g (approximately 0.4mol) is placed in a three-necked flask with a low temperature thermometer, cooled to -10~-5℃ in an ice salt water bath, and 55g (approximately 1.2mol) of absolute ethanol is added dropwise under vigorous stirring, strictly Control the dropping rate so that the temperature of the reaction liquid is always at -30 to 0°C. After the addition, the reaction was continued at -20~10°C for 2h. After the reaction is complete, wash the reaction solution (100ml×2) with distilled water at (0±5)℃, separate the oil layer and use anhydrous Na 2 SO 4 After drying, distillation under reduced pressure with a water pump, collecting 65-75°C fractions, obtaining 51.8 g of colorless and transparent oily liquid (yield 72.3%, calculated as phosphorous trichloride).
[0077] 2...
Example Embodiment
[0110] Example 3
[0111] 1. The synthetic method of triazophos artificial hapten is as follows:
[0112] 1) Synthesis of O-ethylthiophosphoryl dichloride (TZLM-1)
[0113] Weigh phosphorus trichloride (PSCl 3 ) 68g (approximately 0.4mol) is placed in a three-necked flask with a low temperature thermometer, cooled to -10~-5℃ in an ice salt water bath, and 92g (approximately 2.0mol) of absolute ethanol is added dropwise under vigorous stirring, strictly Control the dropping rate so that the temperature of the reaction liquid is always -30 to 0°C. After the addition, the reaction was continued at -20~10°C for 5h. After the reaction, wash the reaction solution (100ml×2) with distilled water at (0±5)℃, separate the oil layer and use anhydrous Na 2 SO 4 After drying, distillation under reduced pressure with a water pump, collecting 65-75°C fractions to obtain 57.0 g of colorless transparent oily liquid (yield 79.53%, calculated as phosphorous trichloride).
[0114] 2) Synthesis of O-et...
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