Method for preparing negative film of antigen of neutrophilic granulocyte

Abstract

This invention relates to the preparation of neutral granulocyte antigen negative used to detect cytoplasmic antigen of neutrophilic granulocyte. It uses heparin anticoagulant method to subside erythrocyte and preserve leucocyte. Then it uses Ficoll-Hypaque gradient reagent to separate neutrophilic granulocyte. Then put in NaCl solution and it becomes cell suspension. Put the suspension directly on to the slide and a neutrophilic granulocyte antigen negative is ready. This negative's detectable rate is obviously greater than other domestic negatives. It has little difference with the import reagent and the cells has only one single deck and is evenly distributed. The result is easy to observe ,so it can meet clinic demand.

Description

Technical field [0001] The invention relates to a preparation method of neutrophil antigen negative film, which is used for detection of anti-neutrophil cytoplasmic antibody. Background technique [0002] Anti-neutrophil cytoplasmic antibody (anti-neutrophil cytoplasmic antibody, ANCA) is an autoantibody that targets neutrophil and monocyte cytoplasmic components. An extremely important serological diagnostic tool for primary vasculitis. Primary vasculitis mainly refers to Wegener's granulomatosis (WG), microscopic polyangiitis (MPA) and necrotizing crescentic glomerulonephritis (NCGN), and their pathogenesis is closely related to ANCA, so clinically The above-mentioned disease is called ANCA-associated vasculitis. ANCA can be divided into cANCA and pANCA according to the different fluorescent staining models. The current research believes that cANCA is more common in WG, while pANCA is often related to MPA and NCGN. They can reflect disease activity, assist diagnosis, gui...

AI Technical Summary

Problems solved by technology

[0006] In the preparation process of the above-mentioned neutrophil antigen negative film, cytocentrifuge spinning is a key step. The expected effect cannot be achieved after using a domestic cytocentrifuge, and the prepared neutrophil antigen negative film is often due to cell It is in a shrunken state, and the cytoplasmic components are not fully displayed, which directly affects the observation of the results, and the price of imported cytocentrifuges is very expensive (about 100,000 yuan). Therefore, most of the commercial neutrophil antigen negatives currently used are imported products. , the price is expensive, and the detection cost is too high, which greatly restricts its routine clinical application