Epiderm substitute for tissue engineering and its prepn process
A technology of tissue engineering and substitutes, which is applied in the field of tissue engineering epidermal substitutes and its preparation, can solve the problems of lack of air permeability, affect clinical repair effect, and reduce air permeability, achieve good air permeability, increase clinical repair effect, The effect of easy access to raw materials
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example 1
[0018] Tissue engineered epidermis substitute prepared by example 1 chitosan-gelatin film material
[0019] A preparation method of tissue engineering epidermis substitute, comprising the following steps: (1) dissolving chitosan with 1% acetic acid to prepare a chitosan solution with a concentration of 2%; (2) dissolving gelatin in distilled water to prepare into a concentration of 4% gelatin solution; (3) the chitosan solution and the gelatin solution were mixed in a weight ratio of 1:9, and stirred and mixed for 24 hours at 37° C.; (4) the mixed solution was added to a flat dish, Heat at 35°C for 2 hours, and dry the film material; (5) Take out the film material from the flat dish, soak the film in absolute ethanol for 20 minutes, and remove the ethanol; (6) Add 30mM EDC to react for 3 hours, and carry out exchange (7) Rinse with a large amount of distilled water and soak for 24 hours; (8) Heat at 35°C for 2 hours and dry to form a film again; (8) Make a film with a diameter...
example 2
[0020] Tissue engineered epidermis substitute prepared by example 2 chitosan-gelatin film material
[0021] A preparation method of tissue engineering epidermis substitute, comprising the following steps: (1) dissolving chitosan with 1% acetic acid to prepare a chitosan solution with a concentration of 2%; (2) dissolving gelatin in distilled water to prepare into a concentration of 4% gelatin solution; (3) chitosan solution and gelatin solution were mixed in a weight ratio of 1:1, and stirred and mixed for 24 hours at 37°C; (4) the mixed solution was added to a plate, and Heating at 48°C for 2.5 hours, drying the film-forming material; (5) soaking the film in absolute ethanol for 20 minutes to remove the ethanol; (6) adding 0.25% glutaraldehyde solution to react for 3 hours to carry out cross-linking reaction; (7) ) and then washed with 2% sodium borohydride solution to remove excess glutaraldehyde, then rinsed with a large amount of distilled water, and soaked for 24 hours; (...
example 3
[0022] Tissue engineered epidermis substitute prepared by example 3 chitosan-gelatin film material
[0023] A preparation method of tissue engineering epidermis substitute, comprising the following steps: (1) dissolving chitosan with 1% acetic acid to prepare a chitosan solution with a concentration of 2%; (2) dissolving gelatin in distilled water to prepare into a concentration of 4% gelatin solution; (3) chitosan solution and gelatin solution were mixed in a weight ratio of 9: 1, and stirred and mixed for 24 hours at 37°C; (4) the mixed solution was added to a plate, and Heat at 60°C for 3 hours to dry the film-forming material; (5) Soak the film in absolute ethanol for 20 minutes to remove the ethanol; (6) Add 30mM EDC to react for 3 hours to carry out cross-linking reaction; (7) Then use Rinse with a large amount of distilled water and soak for 24 hours; (8) heat at 60°C for 3 hours, and dry again to form a film; (8) punch the film with a diameter of 50 microns and a densi...
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