Epiderm substitute for tissue engineering and its prepn process

A technology of tissue engineering and substitutes, which is applied in the field of tissue engineering epidermal substitutes and its preparation, can solve the problems of lack of air permeability, affect clinical repair effect, and reduce air permeability, achieve good air permeability, increase clinical repair effect, The effect of easy access to raw materials

Inactive Publication Date: 2006-03-01
上海蓝融股权投资基金管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Yet the chitosan-gelatin film material that uses in this patent is a kind of dense material, does not have air permeability, especially after adding cells, in wet state, chitosan/gelatin sw...

Method used

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  • Epiderm substitute for tissue engineering and its prepn process

Examples

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example 1

[0018] Tissue engineered epidermis substitute prepared by example 1 chitosan-gelatin film material

[0019] A preparation method of tissue engineering epidermis substitute, comprising the following steps: (1) dissolving chitosan with 1% acetic acid to prepare a chitosan solution with a concentration of 2%; (2) dissolving gelatin in distilled water to prepare into a concentration of 4% gelatin solution; (3) the chitosan solution and the gelatin solution were mixed in a weight ratio of 1:9, and stirred and mixed for 24 hours at 37° C.; (4) the mixed solution was added to a flat dish, Heat at 35°C for 2 hours, and dry the film material; (5) Take out the film material from the flat dish, soak the film in absolute ethanol for 20 minutes, and remove the ethanol; (6) Add 30mM EDC to react for 3 hours, and carry out exchange (7) Rinse with a large amount of distilled water and soak for 24 hours; (8) Heat at 35°C for 2 hours and dry to form a film again; (8) Make a film with a diameter...

example 2

[0020] Tissue engineered epidermis substitute prepared by example 2 chitosan-gelatin film material

[0021] A preparation method of tissue engineering epidermis substitute, comprising the following steps: (1) dissolving chitosan with 1% acetic acid to prepare a chitosan solution with a concentration of 2%; (2) dissolving gelatin in distilled water to prepare into a concentration of 4% gelatin solution; (3) chitosan solution and gelatin solution were mixed in a weight ratio of 1:1, and stirred and mixed for 24 hours at 37°C; (4) the mixed solution was added to a plate, and Heating at 48°C for 2.5 hours, drying the film-forming material; (5) soaking the film in absolute ethanol for 20 minutes to remove the ethanol; (6) adding 0.25% glutaraldehyde solution to react for 3 hours to carry out cross-linking reaction; (7) ) and then washed with 2% sodium borohydride solution to remove excess glutaraldehyde, then rinsed with a large amount of distilled water, and soaked for 24 hours; (...

example 3

[0022] Tissue engineered epidermis substitute prepared by example 3 chitosan-gelatin film material

[0023] A preparation method of tissue engineering epidermis substitute, comprising the following steps: (1) dissolving chitosan with 1% acetic acid to prepare a chitosan solution with a concentration of 2%; (2) dissolving gelatin in distilled water to prepare into a concentration of 4% gelatin solution; (3) chitosan solution and gelatin solution were mixed in a weight ratio of 9: 1, and stirred and mixed for 24 hours at 37°C; (4) the mixed solution was added to a plate, and Heat at 60°C for 3 hours to dry the film-forming material; (5) Soak the film in absolute ethanol for 20 minutes to remove the ethanol; (6) Add 30mM EDC to react for 3 hours to carry out cross-linking reaction; (7) Then use Rinse with a large amount of distilled water and soak for 24 hours; (8) heat at 60°C for 3 hours, and dry again to form a film; (8) punch the film with a diameter of 50 microns and a densi...

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Abstract

The present invention relates to biological material and tissue engineering technology, and is especially one kind of tissue engineering epiderm substituent and its preparation process. The tissue engineering epiderm substitute features that the chitosan-gelatin film or rack possesses pores of 20-50 micron size. Compared with available technology, the present invention adopts chitosan and gelatin as the material for the cell carrying system as the cell substituent, and has the features of easy-to-obtain material, simple technological process, high ventilation and raised clinical repair effect.

Description

[technical field] [0001] The invention relates to the technical field of biological materials and tissue engineering, in particular to a tissue engineering epidermis substitute and a preparation method thereof. [Background technique] [0002] The skin is the largest organ of the human body and plays an important role in protecting the body. When skin defects are caused by various reasons, it will cause obstacles to the function of the body, and even life-threatening. For skin tissue defects, the common clinical treatment methods are vaseline gauze and split skin graft or full-thickness skin graft, among which split skin graft is the recognized standard treatment method. However, the problems of causing new skin wounds and shortage of donors for this treatment method have not been properly resolved so far. [0003] After skin defect, the hypertrophic scar at the defect site is an important complication. Studies have shown that if the skin defect can be quickly epithelializ...

Claims

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Application Information

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IPC IPC(8): A61L27/60
Inventor 丁勃元麻锦央
Owner 上海蓝融股权投资基金管理有限公司
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