IgG detection reagent for mixed agglutination reaction of sperm membrane surface antibody, and immuno-microsphere preparation method

A technology of mixing immune microspheres and antibodies, used in measuring devices, instruments, scientific instruments, etc., can solve the problems of difficulty in purchasing, different IgG sizes, and expensive reagents, and achieve the effect of high cross-linking rate and large number of

Inactive Publication Date: 2010-06-16
深圳华康生物医学工程有限公司
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Problems solved by technology

However, the reagents for the diagnostic method recommended by WHO are expensive and difficult to purchase, and in the MAR detection method, due to the different sizes of IgG, it is difficult to observe the test results, and there are also false positive sperm in the test, which makes the accuracy and reliability of the test low.

Method used

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Embodiment Construction

[0030] The present invention will be described in further detail below through specific examples.

[0031] A sperm membrane surface antibody (MAR) IgG detection reagent comprises phosphate buffer solution containing immune microspheres, antiserum freeze-dried powder and antiserum lysate.

[0032] Wherein the immune microsphere plays the role of antibody carrier, and what described immune microsphere adopts in the present invention is the carboxylated polystyrene latex that combines human IgG, and the diameter of this immune microsphere is 1.8um~2.2um, and its The volume content in the buffer solution is 1-2%. The phosphate buffer can also be replaced with other common buffers with a pH of about 7.4 (pH 7.2-7.6) to maintain the pH value and ionic strength of the environment where the human IgG bound on the immune microspheres resides.

[0033] The diameter of 1.8um~2.2μm is to ensure that the size of the immune microspheres is consistent. At the same time, the immune microsphe...

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Abstract

The invention discloses a semen film surface antibody mixing agglutination reaction IgG testing agent which comprises antiserum and immunity micro ball, wherein the dilemma of the immunity micro ballis 1.8um to 2.2um. The invention also provides an immunity micro ball preparing method which comprises the following steps: 1) washing the stabilizing component of the carboxylation rubber latex, wherein the dilemma of the immunity micro ball is 1.8um to 2.2um; 2) activating the carboxylation group on the surface of the carboxylation rubber latex; 3) coating human on the activated group of the surface of the carboxylation rubber latex; 4) sealing the carboxylation group which is uncombined with the antibody on the surface of the carboxylation rubber latex to obtain the immunity micro ball.

Description

technical field [0001] The invention belongs to the category of in vitro diagnostic reagents, and is particularly suitable for the detection of antisperm antibodies on the surface of motile sperm membranes. Background technique [0002] Immune factors are one of the causes of male infertility. The binding of sperm antibodies to the sperm surface before ejaculation is the only way in which sperm antibodies interfere with male reproduction. The interference mechanism is as follows: ①interfering with the metabolic activation of sperm; ②reducing the number of sperm entering the fertilization site; ③interfering with fertilization; ④inhibiting zygotic cell division. The main basis for the diagnosis of male immune infertility is to confirm the attachment of antibodies on the surface of ejaculated sperm, so the detection of antibodies on the surface of live sperm membranes is the most valuable indicator of immune infertility. [0003] At present, enzyme-linked immunoassay (ELISA) ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/546G01N33/531
Inventor 傅剑华刘瑜胡家纯何林何小红
Owner 深圳华康生物医学工程有限公司
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