Production of L phenylalanine gene engineering bacteria and construction method and its application

A technology of genetic engineering bacteria and phenylalanine, applied in the field of preparation of L-phenylalanine, can solve the problem of high cost
CN1757737AInactive Publication Date: 2006-04-12NANJING UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING UNIV OF TECH
Publication Date
2006-04-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

A genetically engineered bacterium for preparing L-Phe is prepared from transaminase gene and constitutive promoter through linking, and inserting them into carrier to configure constitutive expression plasmid. The L-Phe can be prepared through converting the phenylphruvic acid in proper host bacteria by relative said genetically engineered bacterium. It has high mole conversion rate.
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Description

technical field

[0001] The invention belongs to the technical field of amino acid preparation, and in particular relates to a method for preparing L-phenylalanine using constitutively activated genetic engineering bacteria. Background technique

[0002] L-phenylalanine (English name L-phenylalanine) is a physiologically active aromatic amino acid. It is one of the essential amino acids that cannot be synthesized by humans and animals. It is widely used in medicine. For example, L-phenylalanine is a certain amino acid Intermediates of anticancer drugs, such as: amphetamine, sarcoid formic acid, etc. (Wang Duoren. Chemical Intermediates 2004, 1(2): 12~20). Amino acids can also be used as carriers to load anticancer drug molecules or groups into the tumor area to achieve the purpose of not only inhibiting tumor growth, but also reducing the toxicity of tumor drugs (Janet T Powell et al., Eur.J Biochem.1978, (87 ): 391~400). L-phenylalanine is also an important component of co...

Claims

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