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Bacillus strain and its uses

A technology of Bacillus and Bacillus licheniformis, applied in the direction of bacteria, enzymes, biochemical equipment and methods, etc., can solve problems such as fermentation characteristics of enzyme-producing bacteria, research on fermentation costs of nutritional characteristics, toxicity and safety, low enzyme production levels, etc. , to achieve good market application value, reduce production costs, and produce high levels of enzymes

Inactive Publication Date: 2006-05-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, microorganisms are also used to produce elastase, but the existing elastase-producing bacteria have obvious toxicity and safety problems, and the enzyme production level is generally low, and the fermentation characteristics, nutritional characteristics and fermentation costs of the enzyme-producing bacteria have not been studied. systematic research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Put 25ml of fermentation medium in a 250ml shake flask, seed inoculum amount 4%, seed age 16-18 hours, fermentation temperature 37°C, shaker speed 200rpm, fermentation medium refer to the above, take samples at different times to determine its enzyme activity Variety. The specific values ​​of the enzyme production results at different times in the shake flask fermentation culture are shown in Table 1:

[0048] Fermentation time (h)

[0049] 12

Embodiment 2

[0051] In this embodiment, the culture medium is composed of agricultural and sideline products as raw materials for fermentation and cultivation, and the specific examples of shake flask fermentation and cultivation are described below. The specific values ​​of the fermentation results of shaking flask fermentation with coarse material as the substrate are shown in Table 2. As can be seen from the results, using coarse material as the fermentation substrate can obtain a higher enzyme production level, which can reach the level of fermentation using glucose and casein. The enzyme production level of the substrate is about 70%, and at the same time, the cost of fermentation raw materials can be significantly reduced.

[0052] Seed culture method: 20ml of seed culture medium in 250ml triangular bottle, connected with a circle of activated seeds on the slant, and cultivated at 37°C for 18h.

[0053] The composition of the fermentation medium is as follows (g / 100mL): 3.42% bran, 9...

Embodiment 3

[0057] Seed culture method: 20ml of seed culture medium in 250ml triangular bottle, connected with a circle of activated seeds on the slant, and cultivated at 37°C for 18h. The specific values ​​of the enzyme production results of the 5-L fermenter fermentation culture are shown in Table 3.

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Abstract

The invention discloses a bacillus strain, which is renamed as Bacillus licheniformisú¼with its preservation strain name: Bacillus licheniformis ZJUEL31410ú¼ preservation unit: Chinese microbe cultrue preservation management committee common microbe center, preservation date: 2005-6-23, preservation number: CGMCC 1397. Said bacillus strain is used to produce elastase, whichi is used for the rejuvenation of animal meat, or the hydrolysis of silkworm chrysalis or colubilized silk.

Description

technical field [0001] The present invention relates to a bacillus strain and the application of the strain. Background technique [0002] Elastase is a broad-spectrum proteolytic enzyme characterized by decomposing insoluble elastin, which has great economic value and application value. In recent years, elastase is mainly used in industries such as medicine, food and daily chemical industry. Elastase is mainly used to treat hyperlipidemia, prevent arteriosclerosis, and improve lipid protein metabolism. [0003] my country is a big silk producing country, and silkworm chrysalis is the main by-product of the silk industry, and about 1 ton of dry chrysalis can be obtained for every 1 ton of raw silk produced. Due to the rich nutritional content of dried pupa, it has high health value, and after separation and extraction, it can be used in food, medicine and other industries, and has extremely high economic value. However, the utilization of silkworm chrysalis can not be sol...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/66C12P21/06C12R1/07
Inventor 何国庆陈启和
Owner ZHEJIANG UNIV
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