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Glabrous greenbrier total saponin extract and its prepn and use

A technology of total glucosides of Smilax cocos and its extraction method, which is applied in the field of total glycosides of Smilax cocos and its preparation and application, and achieves the effect of simple extraction process

Active Publication Date: 2006-05-17
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above research is based on the water extract of Smilax cocos and its component astilbin, but there is no report on the extraction process and activity of the total glycosides of Smilax cocos

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the extraction method of Smilax cocos glycosides extract comprises the following steps:

[0026] 1. Carry out percolation extraction to Smilax smilax with 8 times the weight of Smilax smilax, concentration is 60% ethanol aqueous solution, combine ethanol aqueous solution extract, extraction time is 5 hours;

[0027] 2. Recover the aqueous ethanol extract and concentrate it to contain 2 grams of crude drug per milliliter;

[0028] 3. Use ethyl acetate equivalent to 1.0 times the volume of the crude drug to extract 4 times, and combine the extracts;

[0029] 4. Recover the ethyl acetate extract and dry it by vacuum drying at 60° C. to obtain the total glycoside extract.

[0030] After testing, in the total glycosides extract of Smilax cocos, the content of the total glycosides of Smilax cocos extract is 58% (weight), and the content of astilbin is 28% (weight).

Embodiment 2

[0031] Embodiment 2: the extraction method of Smilax cocos glycosides extract comprises the following steps:

[0032] 1. Carry out percolation extraction to Smilax smilax with 6 times the weight of Smilax smilax and 95% ethanol aqueous solution, combine the ethanol aqueous extract, and the extraction time is 2 hours;

[0033] 2. Recover the aqueous ethanol extract and concentrate it to contain 6 grams of crude drug per milliliter;

[0034] 3. Extract 5 times with ethyl acetate equivalent to 2 times the volume of the crude drug, and combine the extracts;

[0035] 4. Recover the ethyl acetate extract and dry it by vacuum drying at 80° C. to obtain the total glycoside extract.

[0036] After testing, in the total glycosides extract of Smilax cocos, the content of the total glycosides of Smilax cocos extract is 66% (weight), and the content of astilbin is 27% (weight).

Embodiment 3

[0037] Embodiment 3: the extraction method of Smilax cocos glycosides extract comprises the following steps:

[0038] 1. Carry out percolation and extraction to Smilax smilax with 12 times the weight of Smilax smilax, concentration is 30% ethanol aqueous solution, combine ethanol aqueous solution extract, extraction time is 12 hours;

[0039] 2. Recover the aqueous ethanol extract and concentrate it to contain 5 grams of crude drug per milliliter;

[0040] 3. Extract 3 times with ethyl acetate equivalent to 0.6 times the volume of the crude drug, and combine the extracts;

[0041] 4. Recover the ethyl acetate extract and dry it in a vacuum drying method at a temperature of 70° C. to obtain the total glycoside extract.

[0042] After testing, in the total glycosides extract of Smilax cocos, the content of the total glycosides of Smilax cocos extract is 55% (weight), and the content of astilbin is 24% (weight).

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Abstract

The present invention discloses one kind of glabrous greenbrier total saponin extract and its preparation process and use. The extraction process includes the following steps: 1. percolation extracting glabrous greenbrier with water solution of alcohol and merging the extracted liquid; 2. concentrating the extracted liquid while recovering alcohol; 3. extracting with ethyl acetate for several times and merging the extracted liquid; and 4. drying the extracted liquid to obtain the glabrous greenbrier total saponin extract while recovering ethyl acetate. The glabrous greenbrier total saponin extract contains glabrous greenbrier total saponin in 50-80 wt%, and astilbin in 20-40 wt%. The present invention features the simple technological process, and high glabrous greenbrier total saponin and effective component content in the extract. The glabrous greenbrier total saponin extract has obvious inhibition on various kinds of inflammation reaction and immune response, and may be used in preparing corresponding medicine.

Description

1. Technical field [0001] The invention relates to a traditional Chinese medicine extract, an extraction method and an application of the extract, in particular to an extract of total glucosides of Smilax cocos and its extraction method, and the extract of total glycosides of Smilax cocos is used for treating various inflammations and immune diseases. 2. Background technology [0002] Smilax smilax is the dried rhizome of Smilax glabra Roxb., a plant of the family Liliaceae. It is a commonly used Chinese medicinal material, which has the effects of dehumidification, detoxification, and joint benefit. Indications for syphilis, stranguria, muscle and bone spasms, scrofula, scabies, carbuncles and other diseases. The water extract of Smilax cocos can inhibit the inflammatory reaction mediated by sensitized T lymphocytes; it has significant anti-inflammatory activity for inflammatory reactions such as adjuvant arthritis; for the delayed phase in adjuvant arthritis, that is, T ly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61K36/90A61P1/16A61P3/10A61P9/00A61P19/02A61P29/00A61P37/06A61P37/08
Inventor 陈婷徐强王双燕
Owner NANJING UNIV
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