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Non-porous single dispersed polymer weak cation exchange resin, its preparation method and use

A weak cation exchange and monodisperse technology, which is applied in the field of non-porous monodisperse polymer weak cation exchange resin and its preparation and application, can solve the problems of difficult chemical modification, etc., and achieve easy scale-up of equipment, increase of separation and analysis speed, The effect of easy operation

Inactive Publication Date: 2006-06-14
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Su Tiansheng (Chinese invention patents, CN 1132213A and CN1257891A) prepared monodisperse macroporous cross-linked polystyrene microspheres and monodisperse macroporous and non-porous cross-linked polyvinylpyridine microspheres by "one-step seed swelling polymerization method". The surface of linked polystyrene and cross-linked polyvinylpyridine presents strong hydrophobicity, which will produce irreversible adsorption on biomacromolecules. As a stationary phase, it can only be used in reversed-phase chromatography and size-exclusion chromatography with organic solvents as mobile phases. It is quite difficult to chemically modify macromolecules when used in ion-exchange chromatography using saline as the mobile phase to separate biomacromolecules

Method used

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  • Non-porous single dispersed polymer weak cation exchange resin, its preparation method and use
  • Non-porous single dispersed polymer weak cation exchange resin, its preparation method and use
  • Non-porous single dispersed polymer weak cation exchange resin, its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: The preparation method of 7.0 μm non-porous monodisperse weak cation exchange resin is carried out in the following order:

[0036] 1) Preparation of 3.0 μm monodisperse linear low molecular weight polystyrene seeds:

[0037]Add 20mL St, 0.4g AIBN, 1.0g PVP and 80mL absolute ethanol to a 100mL round-bottomed flask, ultrasonically dissolve, blow nitrogen to remove oxygen, stir and heat in a constant temperature water bath at 70°C, and react for 24 hours. Remove the solvent, wash several times with absolute ethanol, transfer to a conical flask with 0.2% SDS solution for later use, take a small amount and observe its shape and size under a microscope. The product is monodisperse polystyrene microspheres with a particle size of 3.0 μm.

[0038] 2) Preparation of 7.0 μm non-porous monodisperse hydrophilic cross-linked polyglycidyl methacrylate microspheres:

[0039] Accurately transfer 6mL GMA, 6mL EDMA, and 0.27g BPO into a 250mL beaker. After the BPO is dissolv...

Embodiment 2

[0043] Embodiment 2: The preparation method of 5.0 μm non-porous monodisperse weak cation exchange resin is carried out in the following order:

[0044] 1) Preparation of 2.2 μm monodisperse linear low molecular weight polystyrene seeds

[0045] Add 10mL St, 0.2g AIBN, 0.5g PVP and 90mL absolute ethanol to a 100mL round-bottomed flask, ultrasonically dissolve, blow nitrogen to remove oxygen, stir and heat in a constant temperature water bath at 70°C, and react for 24 hours. Remove the solvent, wash several times with absolute ethanol, transfer to a conical flask with 0.2% SDS solution for later use, take a small amount and observe its shape and size under a microscope. The product is monodisperse polystyrene microspheres with a particle size of 2.2 μm.

[0046] 2) Preparation of 5.0 μm non-porous monodisperse hydrophilic cross-linked polyglycidyl methacrylate microspheres

[0047] Accurately transfer 4.5mL GMA, 4.5mL EDMA, 0.18g BPO into a 250mL beaker, after the BPO dissolv...

Embodiment 3

[0051] Example 3: The preparation method of 3.0 μm non-porous monodisperse weak cation exchange resin is carried out in the following order:

[0052] 1) Preparation of 0.6 μm monodisperse linear polystyrene seeds:

[0053] Add 12.0mL St, 0.24g AIBN, 1.5g PVP and 135mL 90% ethanol solution into a 100mL round bottom flask, ultrasonically dissolve, blow nitrogen to remove oxygen, stir and heat in a constant temperature water bath at 70°C, and react for 24 hours. Remove the solvent, wash several times with absolute ethanol, transfer to a conical flask with 0.1% SDS solution for later use, take a small amount and observe its shape and size under a microscope. The product is monodisperse polystyrene microspheres with a particle size of 0.6 μm.

[0054] 2) Preparation of 3 μm non-porous monodisperse hydrophilic cross-linked polyglycidyl methacrylate microspheres:

[0055] Accurately add 1.0 g of 0.6 μm monodisperse polystyrene seeds synthesized in the previous step and 20 mL of 0.1...

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Abstract

A weak cationic exchange resin used for fast separating and purifying the genetic engineering products and protein is prepared through preparing the non-porous single-dispersed hydrophilic microspheres of cross-linked epoxy propyl polymethyl acrylate, and preparing said weak cationic exchange resin. Its advantages are easy regeneration, high resistance to strong acid and strong alkali, high separation speed and high recovery rate of protein.

Description

technical field [0001] The invention relates to a non-porous monodisperse polymer weak cation exchange resin and its preparation method and application. Background technique [0002] The non-porous polymer type high performance liquid chromatography (HPLC) stationary phase has developed rapidly since its appearance in the late 1980s. Compared with porous packing, its main feature is that it eliminates the peak broadening caused by stagnant mobile phase mass transfer, improves the column efficiency and separation effect, and at the same time, because the separated biomacromolecules will not enter the particle, only in the The surface undergoes mass transfer and exchange, so it can be used for rapid separation and analysis of biological macromolecules such as proteins and peptides. [0003] At present, the method of preparing non-porous cross-linked monodisperse microspheres at home and abroad still uses the method of "two-step seed swelling polymerization" invented by Ugelta...

Claims

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Application Information

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IPC IPC(8): B01J39/26
Inventor 龚波林朱金霞
Owner NINGXIA UNIVERSITY
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