Composite collagen nerve ductus for promoting neural regeneration, and method for forming filature from hollow wet process

A technology of nerve conduit and wet spinning, which is applied in the direction of biochemical equipment and methods, catheter, filament/thread forming, etc., to achieve the effect of avoiding loss and promoting repair

Inactive Publication Date: 2006-07-05
DONGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The composite collagen nerve guide of the present invention is prepared by the comp

Method used

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  • Composite collagen nerve ductus for promoting neural regeneration, and method for forming filature from hollow wet process
  • Composite collagen nerve ductus for promoting neural regeneration, and method for forming filature from hollow wet process
  • Composite collagen nerve ductus for promoting neural regeneration, and method for forming filature from hollow wet process

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Experimental program
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Embodiment 1

[0029] The preparation of embodiment 1 composite bovine collagen spinning stock solution

[0030] Cut the bovine Achilles tendon, wash, remove the aponeurosis, quick-freeze, slice, then cool and wash, treat with 0.8% dimethyl sulfoxide alkali solution for 58 hours to degrease, and then mash it with a high-speed tissue grinder with a rotation speed of 12000r / min crushed, centrifuged (4000r / min) for 15min, and removed the supernatant. Add NaCl (2.0M) to soak for 5min, stir evenly, add Tris-HCl (0.05M, pH=7.5) to soak for 30min, stir overnight, centrifuge (4000r / min) for 20min, repeat NaCl / Tris-HCl treatment several times until the supernatant Liquid viscosity is extremely low. Rinse briefly and repeatedly with cold distilled water until NaCl is removed, centrifuge (4000r / min) for 5min, and dry in the air. Add 0.1% pepsin (model 1:3000) with a weight ratio of 8:1, tetrasodium EDTA and 20 times the volume of distilled water, and place it in an incubator at about 37°C for 24 hour...

Embodiment 2

[0033] The preparation of embodiment 2 composite bovine collagen spinning dope

[0034] Cut the beef Achilles tendon, wash, remove the aponeurosis, quick-freeze, slice, then cool and wash, treat with 2% dimethyl sulfoxide alkali solution for 24 hours to degrease, and then mash with a high-speed tissue grinder with a rotation speed of 12000r / min crushed, centrifuged (4000r / min) for 20min, and removed the supernatant. Add NaCl (3.0M) to soak for 5min, stir evenly, add Tris-HCl (0.05M, pH=7.5) to soak for 30min, stir overnight, centrifuge (4000r / min) for 20min, repeat NaCl / Tris-HCl treatment several times until the supernatant Liquid viscosity is extremely low. Rinse briefly and repeatedly with cold distilled water until NaCl is removed, centrifuge (4000r / min) for 5min, and dry in the air. Add 0.4% pepsin (model 1:3000) with a weight ratio of 4:1, tetrasodium EDTA EDTA and 10 times the volume of distilled water, and place it in an incubator at about 37°C for 24 hours to make th...

Embodiment 3

[0037] The preparation of embodiment 3 composite bovine collagen spinning dope

[0038] Cut the beef Achilles tendon, wash, remove the fascia, quick-freeze, slice, then cool and wash, treat with 5% dimethyl sulfoxide alkali solution for 72 hours to degrease, and then mash with a high-speed tissue grinder with a speed of 12000r / min , centrifuge (4000r / min) for 30min, and remove the supernatant. Add NaCl (4.0M) to soak for 5min, stir evenly, add Tris-HCl (0.05M, pH=7.5) to soak for 30min, stir overnight, centrifuge (4000r / min) for 20min, repeat NaCl / Tris-HCl treatment several times until the supernatant Liquid viscosity is extremely low. Rinse briefly and repeatedly with cold distilled water until NaCl is removed, centrifuge (4000r / min) for 5min, and dry in the air. Add 0.3% pepsin (model 1:3000) with a weight ratio of 6:1, tetrasodium EDTA EDTA and 10 times the volume of distilled water, and place it in an incubator at about 37°C for 24 hours to make the collagen After enzym...

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Abstract

A composite neural canal able to promote the regeneration of nerve is prepared from collagen, chitosan and pore-forming agent through preparing the spinning dope, and one-step hollow wet spinning. It features the combination of spinning and pore forming technique.

Description

technical field [0001] The invention relates to a composite collagen nerve conduit capable of promoting nerve regeneration and a hollow wet spinning forming method thereof. The nerve conduit can be implanted in a living body to repair peripheral nerve regeneration. Background technique [0002] The nervous system is one of the most important organs in the human body, which controls the sensory and motor functions of the human body. Various traumas such as compression, stretching, tearing, cutting, and other factors such as ischemia, tumors, etc. will cause partial or complete damage to the nervous system, resulting in loss of function and other neurological diseases. The repair and functional reconstruction of defective nerves is one of the difficult problems to be overcome by human beings. [0003] At present, the repair of defective nerves mainly adopts adventitial anastomosis and perineural anastomosis; autologous and allogeneic nerve transplantation; Adventitial and pe...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/20A61L27/14A61L27/00A61F2/04A61M25/00D01D4/02D01D5/00C12M3/00
Inventor 张菁成立萍王庆瑞谢涵坤
Owner DONGHUA UNIV
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