Huaman plasma DNA quantitative analyser

A technique for quantitative analysis of human plasma
CN1811387AInactive Publication Date: 2006-08-02潘世扬
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
潘世扬
Publication Date
2006-08-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to a quantitative detection analysis method of human plasma DNA level. Said method includes the following steps: selecting artificially-synthetic exogenous DNA series whose extraction efficiency is identical to or similar to that of human plasma DNA, but they are non-homologous, selecting housekeeping gene beta actin as determination gene, inserting artificially-synthetic exogenous DNA into carrier pMD18T and placing it in the bacteria to make culture, making blue and white spot screening, colony PCR identification, enzyme-cutting into linearity, placing the above-mentioned material into plasma, adopting double fluorescent quantitative gene amplification, fluorescent groups are respectively FAM and HEX, extracting known exogenous positive plasmid recovery efficiency, using computation formula to make computation so as to obtain the quantity of plasma DNA.
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Description

technical field

[0001] The invention relates to a DNA measurement method, in particular to a quantitative detection and analysis method for plasma DNA level. Background technique

[0002] Human plasma DNA refers to free extracellular DNA (cell free DNA), also known as circulating DNA, which is always at a relatively constant level and maintains a balance. Only when the human body suffers from various diseases, such as: body inflammation caused by infection, large area tissue damage caused by trauma, failure of tissues and organs, autoimmune diseases and tumors, etc. due to a large number of cell death, nucleic acid is released from the cells This balance will be broken only when a large amount of internal organs enter the blood of the peripheral circulation, resulting in a substantial increase in human plasma DNA levels and abnormal DNA quality.

[0003] Therefore, the quantitative detection and analysis of plasma DNA can be used for the diagnosis and severity assessment of...

Claims

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