Lucid ganoderma fungus with high glycopeptide composite yield, its mutagen breeding method and use

A complex, glycopeptide technology, applied in fungi and other directions, can solve the problems of high cost, difficult to popularize and use, and achieve the effects of convenient operation, shortening production cycle, and improving mutagenesis efficiency.

Inactive Publication Date: 2006-11-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the high cost of preparing ganoderma lucidum glycopeptide complex feed additives in the prior art and is not easy to be popularized and used in the animal breeding industry, and to breed a high-yield glycopeptide complex through protoplast ultraviolet mutagenesis technology. Ganoderma lucidum fungus can be fermented to produce a low-addition feed with clear functions, which can improve animal immunity and di...

Method used

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  • Lucid ganoderma fungus with high glycopeptide composite yield, its mutagen breeding method and use
  • Lucid ganoderma fungus with high glycopeptide composite yield, its mutagen breeding method and use

Examples

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Embodiment 1

[0034] Example 1. Mutagenesis and breeding of Ganoderma lucidum fungi capable of high-yielding glycopeptide complexes of the present invention

[0035] According to the mutagenesis breeding method provided by the present invention, the ganoderma fungi capable of high-yielding glycopeptide complexes are bred by mutagenesis, and the steps are as follows:

[0036] 1) Cultivation of the starting strain CAU55 mycelia

[0037] The ganoderma lucidum fungus CAU55, which was stored at 4°C in this laboratory and purchased from the General Microbiology Center of the China Microbiological Culture Collection Management Committee, with a strain preservation number of CGMCC 5.533, was used as the starting strain;

[0038] The starting strain CAU55 was inoculated on sterilized solid medium A, and solid cultured at 27°C for 10 days; the cultivated strain was inoculated in sterilized liquid medium B, and cultured in shake flasks at 28°C for 4 days, and then the cultured seeds inoculated in ste...

Embodiment 2

[0051] Example 2, Mutation Breeding of Ganoderma lucidum fungi capable of high-yielding glycopeptide complexes of the present invention

[0052] According to the mutagenesis breeding method provided by the present invention, the ganoderma fungi capable of high-yielding glycopeptide complexes are bred by mutagenesis, and the steps are as follows:

[0053] 1) Cultivation of the starting strain CAU55 mycelia

[0054] The ganoderma lucidum fungus CAU55, which was stored at 4°C in this laboratory and purchased from the General Microbiology Center of the China Microbiological Culture Collection Management Committee, with a strain preservation number of CGMCC 5.533, was used as the starting strain;

[0055] The starting strain CAU55 was inoculated on sterilized solid medium A, and solid cultured at 25°C for 10 days; the cultivated strain was inoculated in sterilized liquid medium B, and cultured in shake flasks at 30°C for 3 days, and then the cultivated seeds inoculated in steriliz...

Embodiment 3

[0068] Example 3. Mutagenesis and Breeding of the Ganoderma Fungus of the Present Invention that Can Highly Produce Glycopeptide Complexes

[0069] According to the mutagenesis breeding method provided by the present invention, the ganoderma fungi capable of high-yielding glycopeptide complexes are bred by mutagenesis, and the steps are as follows:

[0070] 1) Cultivation of the starting strain CAU55 mycelium

[0071] The ganoderma lucidum fungus CAU55, which was stored at 4°C in this laboratory and purchased from the General Microbiology Center of the China Microbiological Culture Collection Management Committee, with a strain preservation number of CGMCC 5.533, was used as the starting strain;

[0072] The starting strain CAU55 was inoculated on sterilized solid medium A, and solid cultured at 30°C for 10 days; the cultivated strain was inoculated in sterilized liquid medium B, and cultured in a shake flask at 32°C for 4 days, and then the cultivated seeds inoculated in ste...

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Abstract

The invention involves a kind of Ganoderma Lucidum that can produce saccha-peptide complexes in high yield, the method of mutagenesis and selection, the purpose of it. The name of the fungi is Ganoderma Lucidum according to Systematic nomenclature, held by 2004-11-22 in <the general microbiology center of china culture collection managerial committee >, and the holding number is CGMCC No.1251. The first step of mutagenesis and selection is to culture the Ganoderma Lucidum CAU55 which is bought from <the general microbiology center of china culture collection managerial committee > as the initial strain and the holding number of which is CGMCC 5.533 in solid medium and fluid medium in turn and get the mycelia solution of the initial strain CAU55. The second step is to treat the mycelia solution with enzymolysis and filtration and get the protoplast deposit of the initial strain CAU55. The last step is to treat the protoplast with ultraviolet mutagenesis and regenerative cultivation, in the end getting the Ganoderma Lucidum CAU5501 that can produce saccha-peptide complexes in high yield. This kind of Ganoderma Lucidum is useful for preparing the feed additive of saccha-peptide complexes from liquid fermentation and some microbiological pharmaceutics like immunopotentiator and beneficial biotin.

Description

field of invention [0001] The invention belongs to the field of biotechnology, and in particular relates to a ganoderma fungus capable of high-yielding glycopeptide complexes, a mutation breeding method and application thereof. Background technique [0002] Due to the extensive and long-term use of antibiotics, chemically synthesized drugs, hormones and other growth-promoting health care agents in animal feeding and feed for many years, serious drug residues in livestock and aquatic products, decline in the quality of livestock and poultry products, increased bacterial resistance, and The increase of drug strains has greatly threatened human health and the ecological environment. Due to the above-mentioned many side effects of antibiotics, and consumers' attention to food safety issues in recent years, countries around the world are banning or restricting the use of antibiotics, and accelerating the development and promotion of new safe and efficient green feed additives to ...

Claims

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Application Information

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IPC IPC(8): C12N1/14
Inventor 张日俊鉏晓艳王铮
Owner CHINA AGRI UNIV
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