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Method for detecting micro-satellite marker LJ_liu of Lateolabrax japonicus

A technology of microsatellite markers and detection methods, which is applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve problems such as limiting the research and development of molecular genetics, and achieve the effect of simple methods

Inactive Publication Date: 2006-12-20
OCEAN UNIV OF CHINA
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Problems solved by technology

However, due to the lack of microsatellite markers in perch, the development of its molecular genetics research is limited, so there is an urgent need to obtain microsatellite markers for the research and application of genetic diversity and pedigree authentication of perch

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  • Method for detecting micro-satellite marker LJ_liu of Lateolabrax japonicus

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Embodiment Construction

[0022] The following examples describe in detail the method of the present invention on the microsatellite core sequence DNA molecular genetic marker of the perch LJ_liu. Firstly, the genomic DNA in the blood of the perch was extracted and diluted for use; then, using the microsatellite core sequence contained in the AFLP band of the perch, specific primers were designed at both ends of the sequence; The genomic DNA of individuals in the perch group was amplified by PCR, and the PCR products were detected by denatured polyacrylamide gel electrophoresis to determine the genotype of each individual, that is, to obtain the genetic polymorphism map of perch.

[0023] 1. Extraction of Genomic DNA from Perch: Add 100 μl of perch blood into an Eppendorf tube, then add 500 μl of cell lysate and 5 μl of 20 mg / ml proteinase K, shake gently, and leave overnight at 37°C. Then, 600 μl of phenol chloride: imitation: isoamyl alcohol (25:24:1) mixture was added to the lysed sample, shaken for...

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Abstract

The invention discloses a detecting method of flower-perch micro-satellite mark LJ_liu, which comprises the following steps: extracting gene group DNA in the flower-perch blood and diluting to prepare; utilizing micro-satellite core sequence in the flower-perch AFLP band; designing astopic primer in two ends of sequence; proceeding PCR augmentation for gene group DNA of different geographic geographical coenologs or flower-perch groups through primer; detecting the PCR augmented product; analyzing band in the product; affirming gene typed in each individual to obtain genetic polymorphism atlas; making the marked technology satisfy Mendel's pattern of heredity; displaying codominant inheritance; fitting for genetic mark of different geographic geographical coenologs or flower-perch groups, phylogenetic identification and genetic atlas construction.

Description

technical field [0001] The invention belongs to the DNA molecular genetic marker technology of Lateolabrax japonicus, and relates to a technical method for detecting the Lateolabrax japonicus microsatellite marker LJ_liu, in particular to a detection method of the Lateolabrax japonicus microsatellite marker LJ_liu. Background technique [0002] Lateolabrax japonicus, also known as perch, is a broad-temperate near-shore middle and lower layer fish. It is distributed in the coastal waters of my country, Japan, and North Korea. It is an important species for coastal breeding and export in my country. In order to rationally utilize and protect the germplasm resources of wild perch, some scholars have studied the genetic structure and genetic diversity of perch by using isozyme technology and RAPD technology. However, since both isozyme markers and RAPD markers are dominant genetic markers, the detection efficiency is not as high as co-dominant markers such as microsatellite mark...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 刘云国李八方
Owner OCEAN UNIV OF CHINA
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