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Heat tolerant asafetida mushroom mutant

A technology for asafoetida mushrooms and mutant strains is applied in the field of three high-temperature-resistant edible fungus strains, which can solve the problems of annual production difficulties of asafoetida mushrooms, reduced production or no harvest, and difficulty in fruiting.

Inactive Publication Date: 2007-01-03
曾宪贤 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the artificial cultivation of Ferulae ferulae has strict temperature requirements, and if the temperature of the mushroom bud formation period and the fruiting period is too high, it will make it difficult to form mushroom buds and produce mushrooms, resulting in reduced production or no harvest, thus affecting the annual production of Ferula ferulae. bring difficulties
[0005] At present, the off-season production of Asafoetida is limited by the germplasm. The commercially available Asafoetida is produced through the cold room mode, so the cost is very high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, the ion beam mutagenesis screening of wild Ferula mycelium cells

[0015] Use a sterile inoculation hook to pick a certain amount of fresh mycelium of wild Ferulica ferulae in Qinghe, insert it into a 150ml conical flask filled with glass beads and 30ml sterile water, break up the mycelia as much as possible, and add a single layer of filter paper Filter through a layer of absorbent cotton to prepare a single-cell suspension of mycelia. Add 20% skimmed milk to the mycelia single-cell suspension as a protective agent, pipette 0.2ml mycelia single-cell suspension and spread it on a Φ90mm sterile blank petri dish, and place it in an ultra-clean typhoon to dry , to make a biofilm.

[0016] with different doses of N + Bunch to irradiate the bacterial film, after the treatment, the bacterial film was eluted with 2ml of sterile water, and 0.2ml mycelia single-cell suspension was pipetted with a pipette and spread on the comprehensive plate medium. Under the co...

Embodiment 2

[0023] Embodiment 2, the ion beam mutagenesis of the conidia of wild mushroom ferulae in Qinghe:

[0024] Get a piece of Qinghe wild ferulae ferulae fruiting bodies that have not erupted conidia, place it in a desiccator with a petri dish, let it erupt naturally, after eruption, collect conidia, and collect conidia Wash and dilute with 2ml sterile water to make conidia suspension of certain concentration. Add 20% skimmed milk to the conidia suspension as a protective agent, take 0.2ml of the conidia suspension and apply it to a Φ90mm sterile blank petri dish, place it in an ultra-clean table and air-dry it to form a bacterial film.

[0025] with different doses of N + Bunch to irradiate the bacterial film, after the treatment, the bacterial film was eluted with 2ml of sterile water, and 0.2ml of conidia suspension was pipetted with a pipette and spread on the comprehensive plate medium. Under the extreme temperature condition of mycelia growth, the constant temperature cultu...

Embodiment 3

[0029] Embodiment 3, the cultivation of ferulae mushroom Qinggeli No. 1

[0030] The cultivation process of Ferula ferulae includes several stages of strain slant cultivation, seed bottle and / or fermenter cultivation, bacteria bag cultivation and fruiting cultivation. After being cultivated in a seed bottle and / or a fermenter, the fermented liquid is inoculated into the fungus bag, and cultured at a constant temperature under the optimum temperature condition of the hyphae. After the mycelium is physiologically mature, the fungus is injected into the fruiting water for fruiting culture.

[0031] (1), slant culture: Scrape a small amount of Asafoetida Qinggeli No. 1 (CGMCC 1761) with a sterile inoculation hook, inoculate it on the PDA medium, and cultivate it at 25°C for 5 days;

[0032] (2), seed bottle culture: pick a certain amount of new hyphae cultivated on a slant with a sterile inoculation hook, inoculate into a 250ml shaker flask with 100ml seed culture medium, and cult...

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Abstract

The present invention provides three kinds of heat tolerant asafetida mushroom nutants, including asafetida mushroom Qinggeli No. 1 CGMCC 1761, asafetida mushroom Qinggeli No. 2 CGMCC 1762 and asafetida mushroom Qinggeli No. 3 CGMCC 1763, obtained through ion beam mutagenesis process with Qinghe wild asafetida mushroom. The process of cultivating asafetida mushroom with these heat tolerant asafetida mushroom nutants includes the steps of slant cultivation, seed bottle and / or fermenting tank cultivation, mushroom bag cultivation and mushroom outputting cultivation. Preferably, in the mushroom outputting cultivation step, water solution comprising alpha-naphthylacetic acid 0.0025 wt%, giberellin 0.0025 wt% triacontanol 0.025 wt% and sterilized water for the rest is injected into the bag. The heat tolerant asafetida mushroom nutants have obviously higher growth temperature than wild asafetida mushroom, and this facilitates the production of asafetida mushroom.

Description

technical field [0001] The invention relates to three high-temperature-resistant edible fungus strains, in particular to three mutant strains of ferulae ferulae obtained through ion beam mutagenesis technology with high-temperature resistance and stable genetics. Background technique [0002] Pleurotus ferulae Lenzi (Pleurotus ferulae Lenzi), also known as Pleurotus ferulae and Bailing mushroom, is named as Bailing mushroom. 5-15 cm, initially oblate hemispherical, then gradually flattened, finally concave and flat near the base, with cracked spots on the surface, always white, thicker, elongated gills, slightly dense, light yellow gills, white stipe , 2-6 cm long, tapering toward base. [0003] Wild ferulae mushrooms are mainly distributed in Ili, Tacheng and Altay regions in the desert area of ​​the Jungar Basin in Xinjiang. Among them, the ferulae ferulae in Qinghe County in Altay region are famous at home and abroad for their pure color, delicious taste and high nutriti...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G1/04
Inventor 曾宪贤吕杰陈恒雷吕长武付永前
Owner 曾宪贤
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