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Timing method for measuring micro-protein

A technology of protein and timing method, which is applied in the field of determination of protein content in food, can solve the problems of low sensitivity and expensive instruments and equipment, and achieve the effect of high sensitivity

Inactive Publication Date: 2007-01-03
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the photometric analysis methods are either too complicated, or the sensitivity is not high, and the instruments and equipment used are mostly expensive.

Method used

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  • Timing method for measuring micro-protein
  • Timing method for measuring micro-protein

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Remove impurities from the soybeans bought in the market, grind them into fine powder, accurately weigh 0.01g of 60-mesh soybean powder, add 10mL of buffer solution with pH=1.30 and mix evenly, add 15mL of 1% NaCl solution and stir evenly, extract 1.5 After h, filter, wash the residue with 200mL of 1% NaCl solution for 10 times, set the volume of the filtrate to 1000mL, take an appropriate amount of the solution and centrifuge at high speed for 10min, take 1mL of the clear liquid, and measure the protein content in the extract according to the following detection method:

[0025] Take two 10mL stoppered colorimetric tubes with the same scale, add 1.0mL protein extraction supernatant, 2.0mL 0.02% acidic chrome blue K solution, 1.0mL 2.0mol / L sulfuric acid solution to one of the tubes, and dilute with distilled water to Close to the scale, then quickly add 0.7mL of 10.0g / L potassium bromate solution, dilute with water to the scale, shake well, quickly put it into the 7200 ...

Embodiment 2

[0027] Purchase milk, put the sample in a water bath at a temperature of 20-30°C and slowly shake and mix for 3-4 minutes. Stir the fat separation emulsion and oil layer without foaming. Gently stir to make the milk visibly separate and place in a water bath to raise the temperature to 35-40°C and mix slowly to help the fat separate. When the fat is separated, the temperature should be quickly adjusted to 20 ° C for 1 min, and 1 mL of the solution should be drawn into a centrifuge tube for mixing. Centrifuge for 2min. Take 1mL into a 100mL volumetric flask and dilute to the mark with 0.5% NaCl. The protein content in the extract was determined by the detection method of Example 1.

[0028] Reference values ​​are measured according to the Bradford assay. The measurement method is as follows: Take 0.1 mL of each of the various protein solutions prepared above, add 1 mL of Coomassie Brilliant Blue CBG-250 working solution, oscillate and shake well, and measure the absorbance A ...

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Abstract

A timing method for determining micro-protein content, that is: the Potassium Bromate can oxidize acid Chrome blue K to discolour in the 0.2mol / L vitriol liquor, forms Landolt reaction, the protein produces the sensitive inhibitory effect for the indication reaction and extends the fading time, establishes the determining micro-protein dynamics analysis method according to the above. The longest absorption wavelength is 526 nm (nanometer), the density of the protein with the range of 0-90 mug / 10mL assumes nicer linearity relation.

Description

technical field [0001] The invention relates to a method for quickly measuring trace protein by using kinetic analysis, which can be applied to the determination of protein content in foods such as soybeans and yoghurt. Background technique [0002] Protein is one of the important components of organisms and the material basis of life phenomena. Protein is closely related to nutrition, cell structure, enzyme, hormone, virus, immunity, material operation, genetics, etc. The analysis and determination of protein is the most important work in biochemistry and other related disciplines and product quality inspection. In recent years, with the early completion of the Human Genome Project, bioanalysis technology has entered the post-gene era, and the research on detection methods of biomacromolecules (especially proteins and nucleic acids) has become a frontier field of life science. Due to the importance of protein in life sciences, the research on quantitative analysis of prote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/78G01N21/27
Inventor 李建平熊金平
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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