Reaction box for quick qualitative detecting infection of helicobacter pylori, and application

A technology for qualitative detection of Helicobacter pylori is applied in the application of diagnosis of Helicobacter pylori infection in peptic ulcers, and the field of reaction boxes for qualitative detection of Helicobacter pylori infection can solve the problem of high cost, achieve fast detection speed and rich source of raw materials , Qualitatively accurate effect

Inactive Publication Date: 2007-02-21
SHANDONG UNIV
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] The latest Pronto dry test paper method is currently the mo

Method used

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Examples

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Embodiment 1

[0027] Take 50g of urea (hydrogen eroxide urea), add 5000ml of deionized water and mix well to prepare a urea solution with a concentration of 1%. Add an appropriate amount of phenol red indicator until the solution turns yellow. Put in the Watergate test paper and wait until it is completely soaked. After the test paper is completely soaked, take it out to dry, and cut the test paper into discs for later use.

[0028] 1-5 minutes before use, add 1-2 drops of distilled water into the shallow recess of the reaction plate with Waltgate dry test paper to dissolve the urea and phenol red on the test paper in water; take 1-2 drops of 1% urease solution Add it into the set shallow depression as a positive control hole; take the gastric mucosa sample taken out during gastroscopy detection and put it into the detection shallow depression; the solution turns pink, indicating positive; if the gastric mucosa specimen is positive, it can be qualitative For Helicobacter pylori infection. ...

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Abstract

A kit for quickly determining infection of polyloric spirillum consists of kit body, application reagent prepared from distilled water and 1% of urase solution, plastic reaction plate with round scrobicula on its surface and Huatemen test paper prepared by soaking said paper completely in 1% of urea solution and in phenol red indicator then drying it in the air.

Description

technical field [0001] The invention relates to a reaction box for diagnosing peptic ulcer and its application, in particular to a reaction box for qualitatively detecting Helicobacter pylori infection and its application in diagnosing Helicobacter pylori infection in peptic ulcer. Background technique [0002] Helicobacter pylori (HP) infection is the main cause of chronic type B gastritis and is closely related to non-ulcer dyspepsia, gastric and duodenal ulcers, and gastric cancer. Epidemiological studies have shown that the HP infection rate among asymptomatic or healthy people in China is 39.9-49.6%. [0003] The traditional detection methods of Helicobacter pylori mainly include: one is to detect HP directly from gastric mucosa, including bacterial culture, tissue smear and section staining; the other is urease test or 14 C breath test to diagnose HP infection, the third is immunological methods such as ELISA, etc. In recent years, there are serological detection and ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N33/52C12Q1/10
Inventor 郭拥军刘同宝孙英陈良华
Owner SHANDONG UNIV
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