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Composition containing ginsenoside F1 and EGCG for preventing skin damage

A technology of gallic acid ester and ginsenoside, which is used in skin care preparations, medical preparations containing active ingredients, skin diseases, etc.

Active Publication Date: 2007-03-07
AMOREPACIFIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there is no information about the ability to regulate apoptosis in human epidermal cells by protecting weakly injured cells from undergoing apoptosis and by inducing apoptosis of severely damaged cells, thereby protecting the skin and being easy to use by humans without reports of hazardous substances

Method used

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  • Composition containing ginsenoside F1 and EGCG for preventing skin damage
  • Composition containing ginsenoside F1 and EGCG for preventing skin damage
  • Composition containing ginsenoside F1 and EGCG for preventing skin damage

Examples

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preparation example Construction

[0033] The preparation of the ginsenoside of reference example 1 purification

[0034] 4 liters of methanol containing distilled water was added to 2 kg of red ginseng (KT&G, six-year-old red ginseng) and refluxed three times, then left standing at 15° C. for 6 days. The crude extract was filtered through filter paper and the residue and filtrate were separated by centrifugation. The filtrate was concentrated under reduced pressure. The concentrate was suspended in distilled water and extracted five times with 1 L of ether to remove the pigment. The aqueous part was extracted three times with 500 ml 1-butanol. The resulting 1-butanol fraction was treated with 5% KOH and washed with distilled water, then concentrated under reduced pressure. The obtained 1-butanol extract was dissolved in a small amount of methanol and a large amount of ethyl acetate was added. The obtained precipitate was dried to obtain 70 g of purified ginsenoside.

[0035] Reference Example 2 Preparatio...

Embodiment 1

[0037] The combined treatment of embodiment 1 ginsenoside F1 and EGCG anti-apoptotic effect in HaCaT cells:

[0038] Step 1 Cell Lines and Cell Culture

[0039] The human keratinocyte HaCaT cell line was provided by Dr. N.E. Fusenig (DeutschesKrebsforschungszentrum (DKFZ), Heidelberg, Germany) and cultured in Dulbecco's modified Eagle's medium (DMEM) (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum. 'smedium, Gibco 1210-0038). Culture at 37 °C in the presence of 5% CO 2 Incubation in moist air.

[0040] Step 2 Inhibition of UV-induced apoptosis in HaCaT cells by combined treatment with ginsenoside F1 and EGCG

[0041] Treat the cell line cultured in step 1 with trypsin to obtain a single-cell suspension, and plate 2 × 10 per well 5 The cells were seeded on 6-well microplates and cultured for 24 hours. Next, the medium was freshly replaced with serum-free DMEM and the cells were cultured for an additional 24 hours. Each well of the microplate ...

Embodiment 2

[0046] Embodiment 2 uses ginsenoside F1 and EGCG joint treatment to the inhibition of PARP proteolysis:

[0047] Step 1 Cell Lines and Cell Culture

[0048] The cell lines and their cultures used in this experiment were the same as those used in Step 1 of Example 1.

[0049] Inhibitory effect of combined treatment with ginsenoside F1 and EGCG in step 2 on UV-induced PARP cleavage

[0050] Treat the cell line cultured in step 1 with trypsin to obtain a single-cell suspension, and plate 2 × 10 per well 5 The cells were seeded on 6-well microplates and cultured for 24 hours. Next, the medium was freshly replaced with serum-free DMEM and the cells were cultured for an additional 24 hours. The microplates were then treated with 2 μM ginsenoside F1; 10 μM EGCG; a combination of 2 μM ginsenoside F1 and 10 μM EGCG; 5 μM ginsenoside F1 and 50 μM EGCG, respectively. Ginsenoside F1 was dissolved in 100% ethanol at a 1 / 1000-fold concentration relative to the medium, and EGCG (Sigma) w...

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Abstract

The present invention relates to a skin-care composition containing ginsenoside F1 and EGCG. More particularly, the present invention relates to an inhibitor of UV-induced apoptosis in epidermal cells showing an excellent skin-care effect by the synergistic interaction of the said ginsenoside F1 and EGCG even at low concentrations, and to a method for inhibiting apoptosis in epidermal cells.

Description

technical field [0001] The present invention relates to ginsenoside F1 (20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol) and (-)epicatechin-3- Composition of gallic acid ((-) epigallocatechin-3-gallate, EGCG). More particularly, the present invention relates to a composition containing low concentrations of ginsenoside F1 and EGCG capable of synergistically preventing skin damage caused by ultraviolet rays, and to a method for preventing skin damage. Background technique [0002] Human skin serves as the first protective barrier, protecting vital organs of the body from external stimuli such as temperature or humidity changes, ultraviolet rays, and pollution, and plays an important role in the regulation of biological homeostasis such as thermoregulation. However, the skin is vulnerable to external stimuli as it is exposed to the external environment. Among these external stimuli, ultraviolet light is the main stimulus that causes skin aging and apoptotic cell death in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/63A61Q19/00A61K8/97A61K8/04A61K8/34A61K8/60A61Q17/04A61Q19/08
CPCA61Q19/00A61K2800/59A61K8/046A61K8/63A61Q17/04A61Q19/08A61K8/347A61P17/02A61P17/16A61P43/00A61K8/9789
Inventor 曹始泳姜炳永廉明勋李泰龙张利燮
Owner AMOREPACIFIC CORP
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