Rice Cyp2 gene for increasing plant salt tolerance
A technology of salt tolerance and rice, which is applied in the field of rice Cyp2 gene, can solve the problems of changing the salt tolerance of plants, achieve the effects of improving plant salt tolerance, increasing farmers' income, and overcoming continuous cropping obstacles
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[0020] If the following content involves concentration and content, if there is no special indication unit, it is the mass percentage concentration or content
[0021] Gene acquisition
[0022] The hybrid rice salt-tolerant combination Shanyou 10 and the salt-sensitive combination Liangyou Peijiu were used as materials. The seeds were sown in a petri dish containing 100mM NaCl soaked in filter paper and placed in a 30℃ incubator for germination. The salt solution was changed every day to maintain salt. The concentration is basically the same. After the seedlings grew to 10 days, the leaves of the seedlings of Shanyou 10 and Liangyoupeijiu were collected.
[0023] Use cold acetone / trichloroacetic acid precipitation method (according to the improved method of Salekdeh et al.: Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett JA proteomic approach to analyzing drought-and salt-responsiveness in rice. Field Crop Res, 2002, 76 (2-3): 199~219) Quickly extract total leaf protein, ...
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