Rice Cyp2 gene for increasing plant salt tolerance
A technology of salt tolerance and rice, which is applied in the field of rice Cyp2 gene, can solve the problems of changing the salt tolerance of plants, achieve the effects of improving plant salt tolerance, increasing farmers' income, and overcoming continuous cropping obstacles
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[0020] The following content involves concentration and content. If there is no special indication, the unit is the mass percentage concentration or content
[0021] Gene acquisition
[0022] The hybrid rice salt-tolerant combination Shanyou No. 10 and the salt-sensitive combination Liangyoupeijiu were used as materials, and the seeds were sown on filter paper culture dishes soaked in 100mM NaCl solution, and germinated in an incubator at 30°C. The salt solution was changed every day to keep the salt The concentration is basically the same. After the seedlings grew to 10 days, the leaves of Shanyou 10 and Liangyoupeijiu seedlings were collected respectively.
[0023] Use cold acetone / trichloroacetic acid precipitation method (according to the improved method of Salekdeh et al.: Salekdeh G H, Siopongco J, Wade L J, Ghareyazie B, Bennett J.A proteomic approach to analyzing drought-and salt-responsiveness in rice. Field Crop Res, 2002, 76 (2-3): 199~219) rapid extraction of tot...
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