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Method for preparing horse anti blood serum

A technology of antiserum and horse serum, applied in the direction of antibodies, antibacterial drugs, antiviral agents, etc., can solve the problems of low purity and specific activity of antiserum, high incidence of allergic reactions and serum sickness in horse serum, and reduce allergies Incidence of reaction and serum sickness, effect of improving purity

Inactive Publication Date: 2007-04-18
SHANGHAI SERUM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The disadvantage of the antiserum stock solution obtained by the above method is that the prepared antiserum has lower purity [F (ab) 2 content, specific activity (IU / gp)], and the incidence rate of horse serum anaphylaxis and serum sickness is lower. high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Purification and preparation of tetanus antitoxin:

[0027] 1. Digestion

[0028] Take 400,000 ml of tetanus immune plasma and dilute 3 times with distilled water, adjust the pH to 3.2 with 2N hydrochloric acid, add appropriate amount of gastric enzyme and 0.2% toluene (ml / ml), and digest at 30°C for 1.5 hours.

[0029] 2. The first precipitation and heating treatment

[0030] Add 15 g of ammonium sulfate to each 100 ml of the digestion solution above, adjust the pH to 5.3 with 2N sodium hydroxide; heat to 58°C and keep for 30 minutes. After the heating is completed, cool down to below 45°C as soon as possible, filter, and take the clear liquid.

[0031] 3. The second precipitation

[0032] The above clear liquid was adjusted to pH 7.2 with 2N sodium hydroxide, and 20 g of ammonium sulfate was added per 100 ml; after stirring evenly, it was left still for 30 minutes, filtered, and the precipitate was collected.

[0033] 4. Alum precipitation

[0034] The above prec...

Embodiment 2

[0044] Purification and preparation of anti-rabies serum:

[0045] 1. Digestion

[0046] Take 400,000 ml of anti-rabies immune plasma and dilute 3 times with distilled water, adjust the pH to 3.0 with 2N hydrochloric acid, add appropriate amount of gastric enzyme and 0.2% toluene (ml / ml), and digest at 30°C for 1.5 hours.

[0047] 2. The first precipitation and heating treatment

[0048] Add 15g of ammonium sulfate to each 100ml of the digestion solution above, adjust the pH to 4.8 with 2N sodium hydroxide; heat to 58°C and keep for 30 minutes. After the heating is completed, cool down to below 45°C as soon as possible, filter, and take the clear liquid.

[0049] 3. The second precipitation

[0050] The above clear liquid was adjusted to pH 7.2 with 2N sodium hydroxide, and 20 g of ammonium sulfate was added per 100 ml; after stirring evenly, it was left still for 30 minutes, filtered, and the precipitate was collected.

[0051] 4. Alum precipitation

[0052] The above pr...

Embodiment 3

[0061] The purification method is the same as in Example 1.

[0062] Product name

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PUM

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Abstract

A process for preparing house antiserum (antitoxin) for preventing the anaphylactic reaction caused by foreign protein and the serum disease includes such steps as digesting the immune plasma, ammonium sulfate depositing twice, alum-adsorbing deposition, ultrafiltering, concentrating and adsorptive purifying by chromatography by ion exchange column.

Description

technical field [0001] The invention relates to a preparation method of horse antiserum, in particular to a purification method of horse antiserum. Background technique [0002] Horse antiserum, also known as antitoxin, is an immunoglobulin preparation prepared from the corresponding toxin or toxoid, the immune serum obtained from animals immunized with bacteria and viruses, or plasma, digested with pepsin, and salted out with ammonium sulfate, such as The technology disclosed in the "China Biological Products Regulations". [0003] At present, the preparation method of horse antiserum generally includes the following steps: 1. Dilute the immune plasma of various antiserum with distilled water, and digest the digestive juice with gastric enzyme; 2. Precipitate the digestive juice for the first time and add Warm treatment, add ammonium sulfate to the digestive solution, and adjust the pH value, then heat the digestive solution and keep it, then lower the temperature, filter ...

Claims

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Application Information

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IPC IPC(8): A61K35/16A61K39/395A61P31/12A61P31/04
Inventor 李景玉范志和张浩季冲
Owner SHANGHAI SERUM BIOTECH
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