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Desmethyl tocopherols for protecting cardiovascular tissue

a technology of desmethyl tocopherols and cardiovascular tissue, applied in the field of desmethyl tocopherols for protecting cardiovascular tissue, can solve the problems of increased risk of occlusive thrombosis, failure to normalize, and confusion in data interpretation, so as to achieve convenient delivery and safe delivery of effective doses

Inactive Publication Date: 2001-11-22
OKLAHOMA MEDICAL RES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0037] In practice, the .gamma.-tocopherol (or other desmethyl tocopherols) would be formulated in a manner allowing safe delivery of effective doses to humans. The .gamma.-tocopherol (or other desmethyl tocopherols) can be absorbed orally by mammals and could be used by oral administration. The .gamma.-tocopherol (or other desmethyl tocopherols) could be administered topically to inflamed skin or gum / mouth or other mucosal tissue as a cream or gel, or could be inhaled as an aerosol. The relative stability and lipophilicity of .gamma.-tocopherol (and other desmethyl tocopherols) make these compounds amenable to delivery in numerous possible formulations. Derivatives of .gamma.-tocopherol (or other desmethyl tocopherols) which retain the structure of a phenolic ring lacking a H atom near the --OH group would also be useful as a protectant against nitrative stress in neurodegenerative conditions; intraperitioneal or intravascular administration in appropriate media may also be used when desired.

Problems solved by technology

End-stage disease is characterized by ischemic damage to the heart and major perfused organs, and with increased risk of occlusive thrombus as portions of plaque disintegrate and initiate coagulation cascades.
Initial studies using relatively small populations (<100 subjects) failed to find a correlation between .alpha.-T and vascular disease, although these studies have been criticized for failure to normalize .alpha.T to lipid content, which might confound the interpretation of the data (28).
Data from large-scale, prospective, controlled tocopherol supplementation trials is currently being analyzed and published with somewhat paradoxical results.
The quantitative discrepancies between epidemiological data and intervention studies are disturbing.
While extensive data has been collected on .alpha.-tocopherol as a possibly beneficial molecule in cardiovascular disease, very little data has been collected on .gamma.T.

Method used

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  • Desmethyl tocopherols for protecting cardiovascular tissue
  • Desmethyl tocopherols for protecting cardiovascular tissue
  • Desmethyl tocopherols for protecting cardiovascular tissue

Examples

Experimental program
Comparison scheme
Effect test

example 2

Demonstration of .alpha.KGDH Protection Against Nitrative Stress In Vivo by Gamma Tocopherol

[0041] Rat pups were injected intraperitoneally (I.P.) with .alpha.T or .gamma.T in an olive oil vehicle every other day for 30 days beginning 2 days after birth; control animals received vehicle only. Olive oil was chosen as a vehicle because of the low tocopherol content in this particular vegetable oil. A total of 15 mg tocopherol was delivered to each animal over the 30 day period; animal weight at the end of the period was approximately 90 g for all three groups. One half of each animal group was injected with a septic dose of LPS (2.5 mg / kg, I.P). After 24 H, animals were killed and organs collected. .alpha.KGDH was assayed in heart tissue and tocopherols were measured by HPLC-ECD / PDA. The supplementation paradigm was sufficient to approximately double the heart tissue level of both .alpha.T and .gamma.T [.alpha.T concentration=23.+-.12 ng / mg protein in controls vs. 48.+-.13 ng / mg in .a...

example 3

Demonstration of Endothelial Cell Protection by .gamma.-Tocopherol

[0042] Cultured ECV304 human endothelial cells were exposed to SIN-1 in the presence of 10 .mu.M .alpha.-T, 10 M .gamma.-T or 5 .mu.M of each (FIG. 5). Tocopherols were incubated with the cells for 19 hours prior to addition of SIN-1. Viability was assayed 24 hours later using a standard tetrazolium (MTT) reduction assay. These cells proved very resistant to damage by SIN-1; however, a 5 mM initial concentration of SIN-1 produced approximately 26% toxicity within 24 hours (N=5; FIG. 5). While .alpha.-T had no apparent effect on SIN-1 toxicity, .gamma.-T promoted viability somewhat and the combination of .gamma.-T with .alpha.-T (1:1 molar ratio) completely prevented SIN-1 toxicity (FIG. 5). The data suggest that .gamma.-T may protect cells in a way that .alpha.-T does not.

example 4

.gamma.T Scavenges Reactive Nitrogen Species in Smokers and Hypertensive Individuals

[0043] Smoking is recognized as a major contributing factor to heart disease and .gamma.-T reportedly decreases more than .alpha.-T in smokers. Hypertension is also a strong risk factor for heart disease. We have begun collection of data from "normal" subjects who do not currently have CAD, including smokers and nonsmokers, and hypertensive subjects. 11 of 54 volunteers from the Oklahoma City Veteran's Administration Hospital and the Oklahoma Medical Research Foundation indicated a current smoking habit. Seven subjects indicated a chronic hypertensive condition. As outlined in Table I, .gamma.-tocopherol tended to decrease in hypertensive subjects while nitration products tended to increase in both smokers and hypertensive subjects. This was the trend regardless of whether the tocopherol concentrations were normalized to plasma triglycerides (Table I).

1TABLE I Preliminary statistical data regarding p...

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Abstract

The present invention involves the use of desmethyl tocopherols such as gamma tocopherol for the protection of cardiovascular tissue from nitrative stress. While mechanisms other than scavenging of reactive nitrogen species may be involved, desmethyl tocopherols exhibit significant protection and may be utilized to treat or help prevent cardiovascular particularly arterial vascular disease. The desmethyl tocopherols may be administered dietarily or parenterally when a more direct dosage is desired. Both routes may be utilized together or separately to optimize therapeutic and prophylactic benefits. The lessening of damage induced by reactive nitrogen species leads to the lessening of arterial blockage in thrombosis.

Description

[0001] Priority is claimed from provisional application U.S. Ser. No. 60 / 186,455 filed on Mar. 2, 2000, and incorporated by reference herein.[0002] The present invention relates to concentrated preparations of desmethyl tocopherols, including but not restricted to gamma tocopherol (.gamma.T), which localize to lipid environments in cardiovascular tissue and scavenge reactive nitrogen species (RNS) by virtue of a phenolic structural element lacking one or more methyl substituents on the phenolic ring system. The capability to scavenge RNS imparts cardiovascular protective properties to the compound.[0003] Tocopherols are a class of lipophilic, phenolic compounds of plant origin. The major tocopherol found in mammalian tissue is alpha tocopherol (.alpha.-tocopherol or .alpha.T or vitamin E) FIG. 1, although significant quantities of demethylated (desmethyl) forms (particularly .gamma.-tocopherol or .gamma.T) FIG. 1, are also present. .alpha.-Tocopherol acts as a free radical scavenger...

Claims

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Application Information

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IPC IPC(8): A61K31/355
CPCA61K31/355
Inventor HENSLEY, KENNETH L.FLOYD, ROBERT A.
Owner OKLAHOMA MEDICAL RES FOUND
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