Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mesoscale polynucleotide amplification analysis

a polynucleotide and mesoscale technology, applied in the field of methods and apparatus for conducting analyses, can solve the problems of not fully exploiting the potential of mesoscale structures in the life sciences, not producing data associated with an analytical device, and not fully exploring the potential of using mesoscale devices for the analysis of biological fluids. , to achieve the effect of reducing reagent costs, reducing storage and shipping costs, and reducing costs

Inactive Publication Date: 2006-02-23
WILDING PETER +1
View PDF18 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The potential capability of mesoscale structures has not been exploited fully in the life sciences.
Hung and Weissman reported a study of fluid dynamics in micromachined channels, but did not produce data associated with an analytic device.
The art has not fully explored the potential of using mesoscale devices for the analyses of biological fluids.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mesoscale polynucleotide amplification analysis
  • Mesoscale polynucleotide amplification analysis
  • Mesoscale polynucleotide amplification analysis

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0070] A polymerase chain reaction is performed in the device illustrated schematically in FIG. 11. To perform a PCR analysis to detect a polynucleotide in a cell, a sample cell lysate is added to a buffered solution of Taq polymerase, nucleoside triphosphates, polynucleotide primers and other reagents required for PCR. The cell sample lysate is delivered via the appliance through entry port 16A to PCR reaction chamber 22A. Ports 16A and 16D are closed by means of valves included in the appliance, while port 16B and 16C are open. The microprocessor and temperature control element in the appliance are used to implement a temperature cycle in reaction chamber 22A between 94° C., for polynucleotide dehybridization, and 65° C., for polymerase reaction. After the polymerase chain reaction is complete, port 16C is closed, 16D opened, and the pump in the appliance connected to port 16B used to deliver the sample from. the PCR reaction. chamber 22A through flow channel 20B to the detection ...

example 2

[0071]FIG. 12 depicts schematically a device 10 including substrate 14 used to separate a nucleic acid from a subpopulation of cells in a mixture in a biological fluid sample, and then to perform an assay for a particular nucleotide sequence. Microfabricated on device 10 is a mesoscale flow path 20 which includes a cell separation chamber 22A, a cell lysis chamber 22B, a filter region 24, a PCR reaction chamber comprising sections 22C and 22D, and a fractal detection region 40. The mesoscale flow system 20 is also provided with fluid entry / exit ports 16A, 16B, 16C and 16D. The device is used in combination with an appliance, such as appliance 50, shown in FIG. 6A.

[0072] Initially, the valves in the appliance are used to close ports 16C and 16D, while ports 16A and 16B are open. A sample containing a mixture of cells is. directed to the sample inlet port 16A by the pump 52 in the appliance, and flows through the mesoscale flow path 20 to separation chamber 22A. Chamber 22A contains ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
temperatureaaaaaaaaaa
temperatureaaaaaaaaaa
volumeaaaaaaaaaa
Login to View More

Abstract

Disclosed are devices for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide polymerization reaction. The devices comprise a substrate microfabricated to define a sample inlet port and a mesoscale flow system, which extends from the inlet port. The mesoscale flow system includes a polynucleotide polymerization reaction chamber in fluid communication with the inlet port which is provided with reagents required for polymerization and amplification of a preselected polynucleotide. In one embodiment the devices may be utilized to implement a polymerase chain reaction (PCR) in the reaction chamber (PCR chamber). The PCR chamber is provided with the sample polynucleotide, polymerase, nucleoside triphosphates, primers and other reagents required for the polymerase chain reaction, and the device is provided with means for thermally controlling the temperature of the contents of the reaction chamber at a temperature controlled to dehybridize double stranded polynucleotide, to anneal the primers, and to polymerize and amplify the polynucleotide.

Description

REFERENCE TO RELATED APPLICATIONS [0001] This application is being filed contemporaneously with the following related copending applications: U.S. Ser. No. [Attorney Docket No. UPA001 (8261 / 2)], Mesoscale Detection Structures; U.S. Ser. No. [Attorney Docket No. UPA002 (8261 / 3)], Analysis Based on Flow Restriction; U.S. Ser. No. [Attorney Docket No. UPA003 (8261 / 4)], Cell Handling in Mesoscale Analytical Devices; and U.S. Ser. No. [Attorney Docket No. UPA005 (8261 / 6)], Mesoscale Sperm Handling Devices, the disclosures of which are incorporated herein by reference.BACKGROUND OF THE INVENTION [0002] This invention relates generally to methods and apparatus for conducting analyses. More particularly, the invention relates to the design and construction of small, typically single-use, modules capable of analyses involving polymerase chain reaction (PCR). [0003] In recent decades the art has developed a very large number of protocols, test kits, and cartridges for conducting analyses on b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12Q1/68B01D61/18B01D67/00B01D71/02B01J19/00B01L7/00C12M1/26C12M1/34C12M3/08C40B40/06
CPCB01D61/18Y10T436/25375B01D71/02B01J19/0046B01J19/0093B01J2219/0059B01J2219/00722B01L3/502707B01L3/502746B01L7/525B01L9/527B01L2200/027B01L2200/0647B01L2200/10B01L2200/146B01L2200/147B01L2300/0627B01L2300/0645B01L2300/0681B01L2300/1822B01L2300/185B01L2300/1861B01L2400/0487C12Q1/686C40B40/06B01D2325/028Y10S436/809Y10S436/805Y10S436/806B01D67/003
Inventor WILDING, PETERKRICKA, LARRY J.
Owner WILDING PETER
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com