Production of fatty acid derivatives
a technology of fatty acid derivatives and derivatives, which is applied in the direction of acyltransferases, microorganisms, enzymes, etc., can solve the problems of high cost of petroleum products development, high cost, and high cost of petroleum exploration, and achieve low impurities and/or undesirable contaminants, and clean emission profiles
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example 1
Production of E. coli MG1655 ΔfadE
[0240]This example describes the construction of a genetically engineered microorganism wherein the expression of a fatty acid degradation enzyme is attenuated.
[0241]The fadE gene of E. coli MG1655 was deleted using the Lambda Red (also known as the Red-Driven Integration) system described in Datsenko et al., Proc. Natl. Acad. Sci. USA 97: 6640-6645 (2000), with the following modifications.
[0242]Two primers were used to create the deletion:
Del-fadE-F:(SEQ ID NO: 1)5′-AAAAACAGCAACAATGTGAGCTTTGTTGTAATTATATTGTAAACATATTGATTCCGGGGATCCGTCGACC-3′Del-fadE-R:(SEQ ID NO: 2)5′-AAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCTGTAGGCTGGAGCTGCTTC-3′
[0243]The Del-fadE-F and Del-fadE-R primers were used to amplify the Kanamycin resistance (KmR) cassette from plasmid pKD13 (as described in Datsenko et al., supra) by PCR. The PCR product was then used to transform electrocompetent E. coli MG1655 cells containing pKD46 (described in Datsenko et al., supra). These cel...
example 2
Production of E. coli MG1655 ΔfadE ΔfhuA
[0245]This example describes the construction of a genetically engineered microorganism in which the expression of a fatty acid degradation enzyme and an outer membrane protein receptor are attenuated.
[0246]The fhuA (also known as tonA) gene of E. coli MG1655, which encodes a ferrichrome outer membrane transporter (GenBank Accession No. NP—414692), was deleted from strain E. coli MG1655 D1 of Example 1 using the Lambda Red system described in Datsenko et al., supra, but with the following modifications.
[0247]Two primers were used to create the deletion:
Del-fhuA-F:(SEQ ID NO: 5)5′-ATCATTCTCGTTTACGTTATCATTCACTTTACATCAGAGATATACCAATGATTCCGGGGATCCGTCGACC-3′;Del-fhuA-R:(SEQ ID NO: 6)5′-GCACGGAAATCCGTGCCCCAAAAGAGAAATTAGAAACGGAAGGTTGCGGTTGTAGGCTGGAGCTGCTTC-3′
[0248]The Del-fhuA-F and Del-fhuA-R primers were used to amplify the KmR cassette from plasmid pKD13 by PCR. The PCR product obtained was used to transform the electrocompetent E. coli MG1655 D1 c...
example 3
Production of E. coli MG1655 ΔfadE ΔfhuA ΔpflB ΔldhA
[0251]This example describes the construction of a genetically engineered microorganism in which the expression of an acyl-CoA dehydrogenase, an outer membrane protein receptor, a pyruvate formate lyase and a lactate dehydrogenase are attenuated.
[0252]The pflB gene of E. coli MG1655, which encodes a pyruvate formate lyase (GenBank Accession No. AAC73989), was deleted from E. coli MG1655 DV2 (see, Example 2) using the Lambda Red System according to Datsenko et al., supra, but with the following modifications:
[0253]The primers used to create the deletion strain were:
Del-pflB-F:(SEQ ID NO: 33)5′-GCCGCAGCCTGATGGACAAAGCGTTCATTATGGTGCTGCCGGTCGCGATGATTCCGGGGATCCGTCGACC-3′Del-pflB-R: (SEQ ID NO: 34)5′-ATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCTGTAGGCTGGAGCTGCTTCG-3′
[0254]The Del-pflB-F and Del-pflB-R primers were used to amplify the Kanamycin resistance (KmR) cassette from plasmid pKD13 by PCR. The PCR product was then used to transform...
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