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Magnetic nanomedicine for tumor suppression and therapy

a nanomedicine and tumor technology, applied in the field of magnetic nanomedicine for tumor suppression and therapy, can solve the problems of limited encapsulation rate, limited dosage applied and therapy effectiveness, serious side effects on other organs of the human body, etc., to improve the thermal stability, increase the local medicine concentration, and great economic advantage

Inactive Publication Date: 2012-12-27
CHANG GUNG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The magnetic nanomedicine increases tumor cell killing rates by 20.4% and 22.6% for bladder tumor cells, demonstrating improved therapeutic efficacy and reduced systemic toxicity, with enhanced thermal stability and controlled medicine release.

Problems solved by technology

Presently, Epirubicin (EPI) is widely utilized in therapy of several cancer diseases, however, since it is strongly toxic to the heart, its dosage applied and its therapy effectiveness are rather limited.
Though it has the advantages of avoiding being decomposed quickly in human body, and capable of being released gradually and slowly, hereby prolonging its circulation time in the body, yet its medicine encapsulation rate is restricted due to the particle size of the carrier, and its medicine releasing speed can not be precisely controlled, also its particle size is overly large (about 150-300 nm), thus it can not be concentrated in large quantity around the area of tumor, hereby causing serious side-effects to other organs of the human body.
In addition, presently, since the medicine of the prior art for treating malignant tumor, such as Epirubicin (EPI) is toxic to the heart, therefore, how to make use of a medicine delivery system to slow down the releasing speed of medicine in human body, and reducing its side-effects to various organs, such as heart, is an important task that has to be solved urgently in this field.
Therefore, presently, the design and performance of the magnetic nanomedicine of the prior art is not quite satisfactory, and it has much room for improvements.

Method used

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  • Magnetic nanomedicine for tumor suppression and therapy
  • Magnetic nanomedicine for tumor suppression and therapy
  • Magnetic nanomedicine for tumor suppression and therapy

Examples

Experimental program
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Effect test

embodiment 1

Method of Preparing Magnetic Nanoparticles (MNPs) of Magnetic Nanomedicine of the Present Invention

[0037]Preparation of MNPs: utilize a co-deposition method to prepare magnetic nanoparticles Fe3O4 (MNPs)

[0038]Firstly, add 0.7 gram of FeCl3 (concentration 4.32×10−3 mole), 1.07 gram of FeCl2.4H2O (concentration 6.48×10−3 mole), and 400 mL of double distilled water into a three-neck bottle, and stir it with a magnet in a nitrogen environment for 5 minutes, so as to make FeCl3 and FeCl2.4H2O fully dissolve into water; then add 20 mL NaOH aqueous solution of concentration 0.864 N into the three-neck bottle, and then heat it to a temperature of 80° C. to produce an aqueous solution containing MNPs.

[0039]Separating MNPs: after cooling down the aqueous solution containing MNPs, agitate it in an ultrasonic agitator to make the MNPs distributed uniformly in the aqueous solution; then pour the aqueous solution containing MNPs into a separating funnel, and place a strong magnet outside the funn...

embodiment 2

Method of Producing a SPAnH / MNPs Magnetic Nanocomposite

[0042]Firstly, mix 2 mL of MNPs aqueous solution having concentration of 10 mg / mL, and 0.5 mL of SPAnNa (poly[aniline-co-sodium N-(1-one-butyric acid)aniline]) aqueous solution having concentration of 4.9 mg / mL uniformly into a mix solution, and put this mixed solution into an ultrasonic agitator to make its contents mix uniformly, then drop in HCl of concentration of 0.5 M. In an acidic environment, SPAnNa aggregates to form SPAnH, and the SPAnH envolops MNPs to form a e SPAnH / MNPs magnetic nanocomposite with the core being MNPs, and with the shell being SPAnH. Next, separate the SPAnH / MNPs magnetic nanocomposite from the solution, and disperse it in double distilled water, hereby forming a SPAnH / MNPs magnetic nanocomposite in aqueous solution.

[0043]The observation result of SPAnH / MNPs magnetic nanocomposite using a transmission electronic microscope is as shown in FIG. 1(A), wherein it shows that the diameter of SPAnH / MNPs mag...

embodiment 3

Efficiency of Preparing EPI / SPAnH / MNPs Magnetic Nanomedicine by Making Epirubicin (EPI) of Various Concentrations to React with SPAnH / MNPs Magnetic Nanocomposite, and the Maximum Medicine Bonding Efficiency

[0046]Dissolve 24 mg of EDC (1-ethyl-3-3-(dimethylaminepropyl)carbodiimidehydro chloride) and 27 mg of sulfo-NHS (N-hydroxysulfosuccinimide sodium salt) in 0.5 M MES buffer (2-Morpholinoethanesulfonic acid, pH=6.3) to form a mixed reaction solution. Next, take 0.2 mL of the mixed reaction solution to react with 0.2 mL of SPAnH / MNPs magnetic nanocomposite solution for 30 minutes, and then flush the product with the MES buffer. Then, add 30 μg, 120 μg, 360 μg, or 560 μg of Epirubicin (EPI) medicine and perform medicine bonding reaction in temperature 20 to 25° C., to form EPI / SPAnH / MNPs magnetic nanomedicine, with its structure as shown in FIG. 9. Subsequently, flush off free EPI several times with de-ionized water. Then, determine the quantity of the flushed-off free EPI with HPLC ...

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Abstract

A magnetic nanomedicine for tumor suppression and therapy, comprising: a core, made of magnetic nanoparticles; a shell, encapsulating said core and is made of carboxylated polyaniline (SPAnH); and a tumor suppression medicine Epirubicin (EPI) or Doxorubicin (DOX) covalently bonded onto said shell. Said magnetic nanomedicine is capable of improving its thermal stability, and it can be dissolved uniformly in water, plus its superparamagnetic property, thus it can be guided by an outside magnetic field to concentrate to the site of tumor distribution to increase the local medicine concentration and enhance therapy effect.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to a magnetic nanomedicine for tumor suppression and therapy, and in particular to a magnetic nanomedicine for tumor suppression and therapy, that can be implemented without the need of surgical operation, and that is also capable of increasing local medicine concentration by means of a magnetic field.[0003]2. The Prior Arts[0004]Presently, Epirubicin (EPI) is widely utilized in therapy of several cancer diseases, however, since it is strongly toxic to the heart, its dosage applied and its therapy effectiveness are rather limited. Presently, most of the carrier used to carry EPI is provided by polymer nano-particles, polymer vesicles and liposome of nano-structure. Though it has the advantages of avoiding being decomposed quickly in human body, and capable of being released gradually and slowly, hereby prolonging its circulation time in the body, yet its medicine encapsulation rate is restr...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K9/51A61P35/00A61K31/704B82Y5/00
CPCA61K31/704A61K9/0009A61P35/00
Inventor HUA, MU-YIYANG, HONG-WEICHUANG, CHENG-KENGPANG, SEE-TONGTSAI, RUNG-YWANCHUANG, KUN-LUNG
Owner CHANG GUNG UNIVERSITY