Absorbent paper and use thereof for breast cancer detection

a technology of absorbent paper and breast cancer, which is applied in the field of absorbent paper and use thereof for breast cancer detection, can solve the problems of significant number of paper cuts to the nipple tissue, affecting the clinical utility of nipple aspirate fluid for breast health management, and affecting the diagnosis of early stage breast cancer

Inactive Publication Date: 2013-05-23
ATOSSA THERAPEUTICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0025]In one embodiment, the total protein concentration of each breast is determined and, if the total protein concentration of one sample is greater than 300 ng, the patient is identified for further assessment of breast cancer

Problems solved by technology

The clinical utility of nipple aspirate fluid for helping in breast health management has been hampered over the last 50 years by the current clinical methodology of collecting and measuring the presence of fluid.
In fact, using current technology, up to 50% of all women are non-secretors, that is, they are judged to not produce nipple aspirate fluid (NAF); this, in turn, m

Method used

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  • Absorbent paper and use thereof for breast cancer detection
  • Absorbent paper and use thereof for breast cancer detection

Examples

Experimental program
Comparison scheme
Effect test

example 1

Assessment of Nipple Aspirate Fluid

[0120]This trial was a single-center study involving three (3) healthy, non-pregnant, non-lactating female subjects. Subjects were enrolled in the order of appearance at the clinic.

[0121]The primary trial objective was to determine the percentage of women from age 30 to 65 that produces ductal fluid, as determined by the presence of protein on the nitrocellulose filter when using the MASCT™ device.

[0122]A secondary objective was to evaluate the nipple aspirate fluid cytologically for the presence and type of cells (if any).

[0123]Abbreviations

[0124]Abbreviations used herein include, for example, MAF: Mammary Aspiration Fluid; MASCT™: Mammary Aspiration Specimen Cytology Test; NA: Not Available; ND: Not Done; NR: Not Recorded; and NAF: Nipple Aspirate Fluid.

[0125]Methodology:

[0126]Briefly, a tared nitrocellulose filter was used to collect ductal fluid by just touching it to each nipple (one for each breast). Next, mammary fluid samples were aspirated...

example 2

Cytology in Biological Samples from Mammary Fluid

[0171]This example describes the use of conventional cytological techniques to identify and assess cells obtained from samples described in Example 1. Following collection of the sample on the filter paper, the filter is washed using any suitable buffered wash solution (e.g., phosphate buffered saline)to collect cells, the fluid may be further processed and cells may be collected by, for example, centrifugation in a modified cytology vial. Processed samples are then transferred to the central region of a clean glass microscopic slide, and a cover slip is slid over the sample to spread it along the surface of the slide. The slide is allowed to air dry and then is fixed, for example in absolute alcohol, and stained with standard cytological stains, such as methylene blue, hematoxyln and eosin, or other suitable stain.

[0172]The slides are then examined by light microscopy for evidence of atypical growth of cells and clumps of cells, usin...

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Abstract

Biological samples of mammary fluid or components thereof are obtained using a breast pump device coupled with an absorbent paper or membrane, optionally facilitated by administering oxytocin to the subject. The breast pump device stimulates expression of mammary fluid and provides for collection of diagnostic samples on the absorbent paper or membrane to evaluate breast disease, including cancer. The biological sample may include fluid containing one or more of cells or cellular components, proteins, glycoproteins, peptides, nucleotides or other desired constituents comprising a breast disease marker. Absorbent paper or membrane, and methods relating to the paper or membrane, and a breast pump device are also provided.

Description

CROSS-REFERENCE[0001]This application claims the benefit of U.S. Provisional Application No. 61 / 550,855, filed Oct. 24, 2011, which application is incorporated herein by reference.BACKGROUND OF THE INVENTION[0002]Breast cancer is by far the most common form of cancer in women, and it is the second leading cause of cancer death in humans. Despite advances in diagnosing and treating breast cancer, the prevalence of this disease has been steadily rising at a rate of about 1% per year since 1940. Today, the likelihood that a women living in North America will develop breast cancer during her lifetime is one in eight.[0003]The current widespread use of mammography has resulted in improved detection of breast cancer. Nonetheless, the death rate due to breast cancer has remained unchanged at about 27 deaths per 100,000 women. All too often, breast cancer is discovered at a stage that is too far advanced, when therapeutic options and survival rates are severely limited.SUMMARY OF THE INVENT...

Claims

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Application Information

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IPC IPC(8): G01N33/68C12Q1/06A61B10/00
CPCG01N33/6836A61B10/0041G01N33/683A61B10/0045G01N33/57415C12Q1/06
Inventor QUAY, STEVEN C.CHEN, SHU-CHIH
Owner ATOSSA THERAPEUTICS INC
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