Dual purpose pollenizer watermelons

a pollenizer and watermelons technology, applied in food preparation, biochemistry apparatus and processes, agriculture tools and machines, etc., can solve the problems of poor quality pink-fleshed fruit, low brix value, and fruit that is unsuitable for consumption and marketing

Inactive Publication Date: 2013-09-05
NUNHEMS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0018]“Pollenizer plant” or “pollenizer” refers to the (inbred or hybrid) diploid plant, or parts thereof (e.g. its pollen or scion), suitable as pollenizer for inducing fruit set on triploid plants. A pollenizer plant is, thus, able to lead to good fruit set (and good triploid fruit yield) of triploid plants, by producing an appropriate amount of pollen at the appropriate day-time and for an appropriate period of time, e.g. at least during peak flowering time of the triploid female plants. A good triploid fruit yield is, for example, a yield comparable to the yield obtainable when using Polimax (produced by Nunhems) as pollenizer (see e.g. Example 3).
[0036]Other fruit characteristics can be introduced by traditional breeding methods (see further below) and thereby combined with pollenizers producing small, edible diploid fruits according to the invention. For example plants can be selected which produce small, edible fruits, as described above, with increased or reduced rind thickness, increased or reduced rind brittleness, various skin / rind colors (e.g. light green; dark green; green-striped with narrow, medium or wide stripes; grey types; with or without spotting; Golden yellow) and rind surfaces (e.g. furrowed or smooth surface), flesh structure / flesh firmness, different fruit shapes (elongate, oval, blocky, spherical or round), higher brix content, higher lycopene and / or vitamin content, different sugar:acid ratios, very good fruit flavour, etc. Also the combination of small edible fruits with another flesh color than red is possible, for example genetic determinants for yellow flesh or orange flesh or white flesh may be introduced, e.g. by backcross breeding with another color-type. See Guner and Wehner 2004, Hort Science 39(6): 1175-1182, in particular pages 1180-1181 describing genes for fruit characteristics. Generally important breeding objectives are early maturity, high fruit yield, high internal fruit quality (good uniform color, high sugar, proper sugar:acid ratio, good flavor, high vitamin and lycopene content, firm flesh texture, non-fibrous flesh texture, freedom from defects such as hollow heart, rind necrosis, blossom-end rot or cross stitch and good rind characteristics and cracking-resistance).

Problems solved by technology

Some pollenizers produce diploid fruits which could be marketable, while others produce fruits that are unsuitable for consumption and marketing.
The fruits of Sidekick are very poor quality pink-fleshed and those of SP-1 are white-fleshed and have a low brix value.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Breeding History

[0094]Breeding for the hybrids goes back to 1985, to in-house breeding lines and crosses of these with the variety Allsweet. Selected self pollinations were backcrossed with Tomato Seed OP (Sugar Baby mutant; is gene) and selections were made for red flesh and small seed. Further self pollinations were selected for red flesh, small seeds and agronomic traits in general. Selections were crossed with a line with many male flowers, followed by several selfings and selection for the production of many male flowers and small red fruits.

example 2

Pollenizer Characteristics

2.1—Materials and Methods

[0095]A field trial was conducted in Italy (Sant'Agata Bolognese-BO). Seeds were sown on 7 Apr. 2010 and transplanted into the field on 21 May 2010 (100 cm within the row, 250 cm between the rows). The plot contained 10 plants per line. Fruits were harvested on 26 / 27 Jul. 2010 for evaluation.

2.1.1 Flowering

[0096]The number of open male and female flowers were counted for three plants per line on the flowering date (day 1=24 Jun. 2010), and 8, 15 and 22 days after the flowering date (day 8=2 Jul. 2010; day 15=9 Jul. 2010; day 22=16 Jul. 2010). The mean number of three plants per line was calculated.

2.1.2 Fruits

[0097]1) Average fruit number: at maturity (26 / 27 July) the total number of fruits harvested from two plants was counted and the average fruit number determined.[0098]2) Average fruit weight: mean of the weight of three fruits per line randomly harvested from two plants at maturity[0099]3) Brix: Value is the mean of three readi...

example 3

Use of Pollenizers According to the Invention

3.1—Trial Set-Up

[0112]Three trials were carried out in Spain using pollenizers WH9317x, WH9318x, WH9320x and WH9321x for triploid fruit production on the triploid hybrid variety ‘Fashion’.

Trial 1:

[0113]Location: green house

Trial dimensions: 3600 m2

Transplanting date: 19 Mar. 2010

Harvest date: 16 Jun. 2010

Scheme: pollenizers and triploids were in separate rows, alternating one row of triploid and one row of pollenizer. Distance between rows was 3 meter, distance between plants in a row was 1 meter.

Trial 2:

[0114]Location: open field

Trial dimensions: 2500 m2

Transplanting date: 24 Mar. 2010

Harvest date: 6 Jul. 2010

Scheme: pollenizers and triploids were interplanted in the same rows, with pollenizers making up 25% of the total plants. Distance between rows was 3 meters and distance between plants in a row was 1 meter.

Trial 3:

[0115]Location: open field

Trial dimensions: 1500 m2

Transplanting date: 20 Mar. 2010

Harvest date: 2 Jul. 2010

Scheme: p...

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Abstract

The application relates to the field of plant breeding, in particular watermelon breeding. Provided are diploid watermelon plants (and seeds from which these plants can be grown) which produce small, diploid, red watermelon fruits. Also provided are small, diploid watermelon fruits having an average weight of less than 1.8 kg.

Description

FIELD OF THE INVENTION[0001]The present invention relates to the field of watermelon breeding and watermelon improvement. Provided are new diploid watermelon plants (2n=2x=22) and seeds from which such plants can be grown, which produce very small diploid fruits. The diploid watermelon plants are preferably suitable as pollenizers in triploid watermelon (2n=3x=33) production, whereby the pollenizers therefore have a dual purpose: providing sufficient viable pollen to pollinate female flowers of triploid plants (which after pollination produce triploid, seedless watermelon fruits) and / or to provide small (“mini”- or personal size), edible diploid fruits on the pollinizer plants themselves.BACKGROUND OF THE INVENTION[0002]Seedless watermelon (Citrullus lanatus (Thunb.) Matsum. And Nak.) production involves using pollen from diploid male parent plants to fertilize flowers of tetraploid (2n=4x=44) maternal parent plants. Pollination of the tetraploid flowers with diploid pollen leads to...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A01H5/08A01G1/00A23L1/212A23L19/00
CPCA01H5/00A01H5/08A23L1/212A01G1/001A01H5/0806C12N15/8242A23L19/00A01H6/78A01G22/05A01H6/342A01H5/10A01H1/08
Inventor DE GROOT, ERIKCHIAPPARINO, ELENA
Owner NUNHEMS BV
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