Matrices comprising a modified polysaccharide

Inactive Publication Date: 2014-10-23
ALBERT LUDWIGS UNIV FREIBURG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention relates to a matrix which can be used for regenerative medicine and plastic surgery. The matrix can be made with a controlled ratio of modified polysaccharide to unmodified polysaccharide, which allows for precise control of its mechanical properties. The matrix can be sterilized and brought into contact with cells which form blood vessels or cartilage tissues. It can be used as an artificial skin, blood vessels, nerve tissues, mucosal tissues, or lenses. The matrix has superior optical quality which is important for certain applications. It can also be used for controlled drug release formulations of pharmaceutically active components. The mechanical properties of the matrix can be adjusted depending on the desired purpose.

Problems solved by technology

Nevertheless, most of these intercellular interactions are not fully understood.
(1) Use of animal protein extracts such as Matrigel® which suffer from a lack of batch to batch reproducibility and a poor definition of the components. This leads to difficulties in the interpretation of the results obtained with such ECMs.
(2) Use of synthetic polymers such as degradable polyester that although biocompatible are not easy to synthesize and manufacture and additionally, require knowledge in synthetic chemistry to set it up and are also difficult to translate into in vivo clinical settings.
(3) Use of natural components of the ECM such as collagen or hyaluronic acid, which reproduce only one aspect of the natural ECM environment.

Method used

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  • Matrices comprising a modified polysaccharide
  • Matrices comprising a modified polysaccharide
  • Matrices comprising a modified polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

example 1

Modification of Agarose

[0130]Agarose type I was obtained from Calbiochem. (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl (TEMPO), NaOCl, NaBH4, NaBr, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and 2-(N-morpholino)ethanesulfonic acid (MES) buffer were obtained from Sigma Aldrich and used as received. Solution of 0.5 M NaOH as well as solution of 5 M HCl were freshly prepared every three-month. Ethanol technical grade was used without any further purification. Deionized water was used for non-sterile synthesis.

[0131]Agarose was modified under sterile conditions: all the chemicals were dissolved in autoclaved water and filtered with a 0.2 μm filter. All the glassware was autoclaved and the reaction was conducted under a laminar flow. Agarose (1 g) was autoclaved in MilliQ water. Autoclaved agarose was poured into a 3 necked round bottom flask, which was used as a reactor. A mechanical stirrer was adapted to one of the neck. A pH-meter was adapted another neck of the round bottom flas...

example 2

Modification of K-Carrageenan

[0132]K-carrageenan was obtained from Sigma Aldrich and used as such. K-carrageenan was objected to a TEMPO-mediated oxidation, according to the synthetic protocol of Example 1.

[0133]The resulting modified k-carrageenan was dialyzed and freeze-dried, as specified in Example 1 above.

example 3

Blending of Modified Agarose with Unmodified Agarose

[0134]Blends of modified agarose and native agarose in different proportions were prepared. The resulting blends were studied using CD spectrometry. The obtained results indicate that the blending of two polysaccharides lead to the same change in tertiary structure as the modified polysaccharide. This suggests that these two polysaccharides are miscible and can be used for engineering new matrices.

[0135]The rheology studies show a specific behavior of the gel. Indeed the blended gels have a higher shear modulus than the unmodified gel of agarose. These results suggest that the organization of the modified chains with the unmodified chains follows a new mechanism, that is different from modified agarose.

[0136]The ESEM image illustrates the structure of the gels and reveals a different organization of the fibers than for the unmodified agarose. As well for the surface roughness, the roughness of the blended gels is not dependent of t...

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Abstract

The present invention discloses a matrix comprising a modified polysaccharide consisting of repeating disaccharide units whereby in at least 11% of the disaccharide units one primary alcohol group is oxidized into a carboxylic acid group.

Description

FIELD OF THE INVENTION[0001]The present invention relates to matrices comprising a modified polysaccharide and the applications of such matrices. The matrixes can be used as scaffold for living cells regenerative implants, plastic surgery implants and controlled drug release implants. In addition thereto, the matrixes of the present invention are suitable for use as a food additive, a component for cosmetic compositions and for other industrial purposes.BACKGROUND OF THE PRESENT INVENTION[0002]In the text of the present application, the nomenclature of amino acids and of peptides is used according to “Nomenclature and symbolism for amino acids and peptides”, Pure & Appl. Chem., Vol. 56, No. 5, pp. 595-624, 1984, if not otherwise stated.[0003]The following abbreviations have the meaning as given in the following list, if not otherwise stated:AFM atomic force microscopyCD circular dichroismCSF cell shape factorCT computed tomographyDIC differential interference contrast imagingDLS dyn...

Claims

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Application Information

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IPC IPC(8): C08B37/00C08H1/00A61L31/12A23L1/0532A61K8/73C10M107/36A61Q90/00C08L5/00A61K47/36A23L29/256
CPCC08B37/006C08L5/00C08H1/00A61L31/125A23L1/0532A61K8/73C10M107/36A61Q90/00A61K47/36C08B37/00C08B37/003C08B37/0039C08B37/0042C08B37/0063C08B37/0069C08B37/0072C08B37/0075C08L5/12C12N5/0068C12N2533/50C12N2533/70A23L29/256A61K31/715
InventorSHASTRI, V. PRASADFORGET, AURELIEN
OwnerALBERT LUDWIGS UNIV FREIBURG