Cosmetic use of a carob seed extract as a slimming active agent

a technology of carob seed extract and active agent, applied in the field of cosmetics, can solve the problems of unattractive hyperplasia of body fat mass and more specifically appearance of localized excess body fat, and products generally have moderate or limited efficacy

Inactive Publication Date: 2015-10-22
ISP INVESTMENTS LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If a sustained imbalance occurs in the body promoting lipogenesis, the quantity of lipids stored in the adipocytes increases, leading to hyperplasia of the mass of body fat and more specifically to the appearance of localized excess body fat.
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Method used

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  • Cosmetic use of a carob seed extract as a slimming active agent

Examples

Experimental program
Comparison scheme
Effect test

example 1

Preparation of a Carob Peptide Extract (Ceratonia siliqua L.)

[0091]Carob germ (Ceratonia siliqua L.) in powder form is placed in solution in 70 volumes of water and the pH is adjusted to a value of between 4.5 and 5.5.

[0092]To eliminate insoluble sugars, hydrolysis with a cellulase is performed. For this, 2% CELLUCLAST® CL (Novozymes) and 2% POLYCLAR® 10 (polyvinylpyrrolidone—PVPP—insoluble) are added to the reaction medium. The reaction medium is then heated for two hours at 50° C. then deactivated for one hour at 80° C. A filtration step makes it possible to separate the carbohydrate-rich filtrate so as to preserve only the solid residue.

[0093]The latter is characterized by a protein content of between 45 and 50% and a sugar content of between 20 and 30%.

[0094]The dry residue thus obtained is placed in solution in 100 volumes of water in the presence of 2% POLYCLAR® 10. The mixture is adjusted to a pH of between 8.0 and 8.5 with a 2 M aqueous soda solution.

[0095]To improve the ext...

example 2

Evaluation of the Expression of Aquaglyceroporin 7

[0106]The objective of this study is to determine the influence of the association of the carob extract according to example 1 and caffeine, on the expression of aquaglyceroporin 7 in differentiated 3T3-L1 adipocyte cells.

Protocol:

[0107]Culture and differentiation of 3T3-L1 cells:

[0108]3T3-L1 adipocyte cells are cultivated in a DMEM medium with 4.5 g / l glucose, 2 mM glutamine and 10% fetal bovine serum.

[0109]Two days after the cell confluence phase, the differentiation of 3T3-L1 cells into adipocytes is induced by adding a solution of 0.5 mM of IBMX, 1 μM of dexamethasone and 10 μg / ml of insulin (Sigma, St. Louis, Mo., USA) in the culture medium for 3 days. Then, only the insulin is kept for 3 to 4 days of additional culture. The cells are then kept in culture, in a standard medium, for another 3 days.

[0110]Treatment:

[0111]The treatment is performed from the start of the differentiation induction phase by a daily application, for 12 ...

example 3

Evaluation of Lipid Droplets Contained in Adipocytes

[0119]The objective of this study is to measure the influence of the association of the carob extract according to example 1 and caffeine, on the size and number of lipid droplets contained in the differentiated 3T3-L1 adipocyte cells.

Protocol:

[0120]The culture, the differentiation of 3T3-L1 cells, then the treatment with the compounds to be tested are performed as in example 2.

[0121]Detection of Lipids by Nile Red:

[0122]The lipid detection is performed using Nile Red fluorescent stain, a phenoxazone that intensely labels the intracellular lipids.

[0123]The color of the fluorescence observed is directly dependent upon the hydrophobicity of the surrounding medium. This specific property of Nile Red makes it possible to differentiate neutral lipids, labeled in gold-yellow, from phospholipids, labeled in red.

[0124]The cells are fixed with a 3.7% formaldehyde solution for 10 minutes, then labeled by a solution of 100 nM Nile Red in PBS ...

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Abstract

Cosmetic methods for obtaining a slimming effect are disclosed. The methods include providing a composition containing a carob (Ceratonia siliqua L.) seed extract as a slimming agent and a physiologically acceptable medium, and topically applying the composition on at least a portion of the body or face. The topical application of the composition includes the application of an effective amount of the composition that increases the expression of aquaglyceroporins, promotes lipolysis, and/or reduces localized excess body fat.

Description

FIELD OF THE INVENTION[0001]This invention concerns the field of cosmetics and more specifically the field of cosmetic weight-loss methods. It relates to the cosmetic use of a carob germ extract (Ceratonia siliqua L.) as a slimming agent. The invention also relates to the cosmetic use of the combination of a carob germ extract for increasing the expression of aquaglyceroporins and promoting the elimination of triglycerides contained in adipocytes.[0002]The invention also relates to a method of cosmetic care including the topical application, on at least a portion of the skin of the body or the face, of a carob germ (Ceratonia siliqua L.) in a composition containing a physiologically acceptable medium, in order to obtain a slimming effect, and more specifically to reduce localized excess body fat.BACKGROUND OF THE INVENTION[0003]The subcutaneous adipose tissue is located at the hypodermis. It is a type of connective tissue where adipocytes are predominant, organized in lobes around 5...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61K8/64A61Q19/06
CPCA61K8/97A61K8/645A61Q19/06A61K8/9706A61K8/9728A61K8/9789
Inventor BOTTO, JEAN-MARIEDOMLOGE, NOUHAPORTOLAN, FREDERIQUE
Owner ISP INVESTMENTS LLC
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