Liquids rich in noble gas and methods of their preparation and use
a noble gas and liquid technology, applied in the field of molecular biology, medicine and nutraceuticals, can solve the problems of fraction of inspired oxygen needed for cell survival, inability to give xe or ar in many situations, and difficulty in developing a continuous inhalation strategy for patients
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example 1
Methods for Studies of Example 2
[0138]Preparation of Xe-Rich-Solution.
[0139]Xenon-rich-solution was composed of olive oil (or can be made with other oils such as flaxseed oil, rapeseed oil, soybean oil, walnut oil, fish oil etc), egg phosphocholine (Avanti, polar lipid. Alabama, USA), BSA (or other protein such as milk), and lithocholic acid (Sigma-Aldrich, St. Louis, Mo., USA). The solution, comprising 25% oil component, was emulsified using a sonication method and stabled by fabrication of the emulsion with surfactants such as phospholipids (egg PC, soybean PC, DPPC, DOPC etc), proteins and lithocholic acid. Xenon (Matheson Tri-Gas®, Houston, Tex., USA) was saturated into oil by pressurization at low temperature.
[0140]Murine Experiment Setting.
[0141]All animal studies were approved by the Animal Welfare Committee at The University of Texas Health Science Center at Houston. C57BL / 6J wild type (WT) and apolipoprotein-E (Apo E) knockout (KO) mice were purchased from Jackson Laborator...
example 2
Xe Administration Studies
[0158]Resistance to Cardiac Hypertrophy in Response to Xenon (Xe) Exposure.
[0159]To examine the effect of Xe activity on heart disease an apolipoprotein E knockout (apoE− / −; or “KO” as used herein) mouse model was employed. This is a well-established model for atheriosclerosis, as the animals will develop atherosclerotic lesions even on a normal chow diet, while a high fat diet significantly accelerates this process (Meir et al., 2004). Accordingly, the model has previously been successfully employed to evaluate the effects of both natural compounds and pharmaceuticals on atheriosclerosis and cardiovascular disease.
[0160]Animals were divided into 5 groups (see experiment setting). Echocardiography was used to assess cardiac dimensions and function at baseline, and at 6-weeks following the Xe exposure (FIGS. 1 and 2; Table 1). Cardiac size of WT and KO hearts were determined with LV mass (corrected) and normalized to body weight (mg / g) following 6-weeks of th...
example 3
Material and Methods for Example 4
Preparation of Caged Molecular Enclosed Xenon
[0174]Xenon was enclosed into a soluble caged molecules (e.g., cyclodextrin). To remove possible residue molecules from cage, the caged molecular was baked at 40-80° C. under vacuum for overnight. To enclose xenon into caged molecules, xenon was incubated with caged molecular in a sealed vial under 2-10 atm pressure at 4 to −180° C. for overnight to 3 days.
Preparation of Pure Xenon Supersaturated Water
[0175]Pure water was degassed under 20-80 mbar vacuum at room temperature for overnight. Xenon (99.999% Medical grade, Matheson Tri-Gas®, Houston, Tex., USA) was re-dissolved into degased water by pressure water with 2-10 atm xenon gas at 4° C. for overnight to 3 days.
Preparation of Xe-Rich-Water
[0176]Xe-rich-water is composed of Xe directly dissolved in water and caging with hydroxypropyl-beta-cyclodextrin (hp-beta-CD) in water. To prepare a Xe-rich-water, Xenon supersaturated water (10 ml) was injected int...
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