Small molecule, indirubin-3-oxime, for prevention and treatment of bone disease

a small molecule, bone disease technology, applied in the direction of skeletal disorder, food preparation, medical preparation, etc., can solve the problems of inconvenient use, no drug has a direct target for height growth, and growth hormone injection is not suitable for a child with normal hormone secretion, etc., to achieve low toxicity, low development cost, and clear

Inactive Publication Date: 2016-09-22
IND ACADEMIC CORP FOUND YONSEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0020]As described above, the composition according to the present invention, comprising an indirubin derivative for stimulating longitudinal bone growth, has an effect of treating or preventing short stature, microplasia, dwarfism, or precocious puberty. In addition, unlike conventional therapeutic agents for growth, ind...

Problems solved by technology

However, most of the drugs that are currently in the market simply contain nutritional supplement components, and a question remains as to the proportion of therapeutic agents for growth that produce a real effect.
Furthermore, virtually no drug has a target directly related to height growth.
However, growth hormone injection is not suitable for a child with normal hormone secretion, and instead produces side effects when used, such as hypothyroidism and hyperpituitarism.
Moreover, it is not easily accessi...

Method used

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  • Small molecule, indirubin-3-oxime, for prevention and treatment of bone disease
  • Small molecule, indirubin-3-oxime, for prevention and treatment of bone disease
  • Small molecule, indirubin-3-oxime, for prevention and treatment of bone disease

Examples

Experimental program
Comparison scheme
Effect test

example 1

Cell Culture and Reagents

[0026]Rat chondrosarcoma (RCS) cells were cultured in a Dulbecco's modified Eagle's medium (DMEM; Gibco, Grand Island, N.Y.), supplemented with 10% fetal bovine serum (FBS) and penicillin / streptomycin.

example 2

Western Blot Analysis

[0027]For Western blot analysis, cells were treated with indirubin-3′-oxime for 24 hours, cultured and reached to 70% confluency and then were harvested. After the harvested samples underwent cytolysis, the proteins were quantified, which was followed by electrophoresis using 10% or 12% SDA-PAGE gel. Afterwards, immunoblot was conducted using antibodies.

example 3

Immunocytochemistry

[0028]2.5×104 cells were adhered to a cover glass placed in a 12-well plate. Once securely attached, cells were treated with indirubin-3′-oxime for 24 hours, and then were rinsed with a phosphoric acid buffer solution. After adding 4% paraformaldehyde, cells were fixed at room temperature for 20 min. Then, proteins expressed in the cells were observed through cell membrane permeation, blocking, and immunofluorescent staining processes.

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Abstract

The present invention provides a composition comprising an indirubin derivative for stimulating longitudinal bone growth. Because the composition for stimulating longitudinal bone growth according to the present invention is shown to be effective in longitudinal bone growth, it may be used not only as a composition for stimulating longitudinal bone growth, but also as a pharmaceutical composition for treating or preventing short stature, microplasia, dwarfism, or precocious puberty.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority to Korean Application No. 10-2013-0081464 filed on Jul. 11, 2013, which is incorporated herein by reference.TECHNICAL FIELD[0002]The present invention relates to a small molecule, indirubin-3-oxime, for prevention and treatment of bone disease.BACKGROUND ART[0003]The increased awareness of the importance of normal growth, as well as an increased aesthetic interest, in infants and adolescents has led to an increased public interest in height growth. In the past, height growth was thought to depend on heredity. However, it is said that the proportion to which heredity is responsible for actual height growth is only 23%, and also, depending on the care during the period of growth, sufficient height growth can be achieved. Therefore, a great deal of money is being invested, estimated to be anywhere between 4-100 million dollars, to achieve height growth within the time period in which the growth plate is not c...

Claims

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Application Information

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IPC IPC(8): A61K31/404A23L2/52
CPCA61K31/404A23V2002/00A23L2/52A23L1/3002A23L33/10A23L33/105A61P19/00A61K31/403
Inventor CHOI, KANG-YELLKIM, HYUN YICHOI, SEHEE
Owner IND ACADEMIC CORP FOUND YONSEI UNIV
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