Rapid acting lactobacillus strains and their use to improve aerobic stability of silage

Abstract

A method for treating silage to enhance the aerobic stability by increasing the fermentation and stabilization of silage by inhibiting growth of microorganisms selected from yeasts, molds and spore-forming bacteria and permitting earlier aerobic exposure is disclosed. The method comprises treating silage or feed with a composition comprising Lactobacillus buchneri strain LN7125, or Lactobacillus brevis strain LB5328, or Lactobacillus brevis strain LB7123, and mixtures or a mutant thereof which retains the silage preservative activity of LN7125, LB5328, or LB7123, or the antimicrobial components produced thereby. The strains of Lactobacillus buchneri and Lactobacillus brevis disclosed in the invention have been purified and isolated and have been found to improve aerobic stability of silage allowing earlier aerobic exposure post ensiling than is presently practiced

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of co-pending U.S. patent application Ser. No. 14 / 200,231, filed Mar. 7, 2014, which is incorporated herein in its entirety by reference.FIELD OF THE INVENTION[0002]The invention relates to compositions and methods of treating animal feed and preserving silage to enhance aerobic stability.BACKGROUND OF THE INVENTION[0003]The ensiling process is a method of moist forage preservation and is used worldwide. Silage accounts for more than 200 million tons of dry matter stored annually in Western Europe and the United States alone. The process involves natural fermentation, where lactic acid bacteria ferment water soluble carbohydrates to form organic acids under anaerobic conditions. This causes a decrease in pH which then inhibits detrimental microbes so that the moist forage is preserved.[0004]Aerobic instability is the primary problem in silage production. Traditionally, the recommendation has been to allo...

AI Technical Summary

Benefits of technology

The invention is about using special bacteria to improve the quality of silage, which is a type of stored forage. These bacteria help to maintain the nutritional value of the silage and make it more stable. The invention includes a mixture of specific bacteria species, such as Lactobacillus buchneri and Lactobacillus brevis, and a carrier substance like calcium carbonate or starch. The bacteria can be isolated and purified, and the carrier can be a liquid or solid. When added to silage, the bacteria help to make it more stable, so it can be exposed to air earlier. This improves the quality of the silage and makes it easier to preserve. The amount of bacteria added can range from 10 million to 10 million colony-forming units (CFU) per gram of silage.

Problems solved by technology

Aerobic instability is the primary problem in silage production.

Even before storage units are open for feedout, silage can be exposed to oxygen because of management problems (i.e., poor packing or sealing).

Under these types of aerobic conditions, rapid growth of yeast and mold cause silages to heat and spoil, decreasing its nutritional value.

Feeding a crop that has not been properly fermented can lower dry matter intake (DMI), decrease milk production, and cause digestive upsets.

Aerobic instability can be a problem even in inoculated silage that has undergone what would traditionally be considered a “good” fermentation: a rapid pH drop, and a low terminal pH.

Production of silage inoculant strains and the ensiling process is complex and involves interactions of numerous chemical and microbiological processes.