Peptides derived from the propeptide ntsr3 and their use in the treatment of depression

a technology of propeptides and peptides, which is applied in the field of short peptides, can solve the problems of depression being a major public health problem, depression is a very common and often debilitating disease, and the economic burden of more than 30 billion euros per year, so as to avoid all undesirable side effects, reduce the time of action, and reduce the effect of long tim

Active Publication Date: 2019-09-05
CENT NAT DE LA RECHERCHE SCI +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0043]The peptides of the present invention as defined above, with the exception of G / A-PE(22-28), are natural products which constitute a new type of antidepressant. They enable avoiding all undesirable side effects of drugs currently used for the treatment of depression. The side effects of the drugs used in the prior art come from their chemical nature. These prior art drugs are molecules that often have the property of passing through cell membranes passively, resulting in nonspecific interactions with intracellular effectors. The peptide nature of the present invention enables avoiding this problem.
[0044]Furthermore, the direct action of a peptide of the present invention on the potassium channel TREK-1 could well reduce the time of action, often long, of a conventional antidepressant.

Problems solved by technology

Depression is a major public health problem.
Depression is a very common and often debilitating disease.
Depression is already one of the leading causes of work stoppage, with an economic burden of more than 30 billion Euros per year.
In addition, the time of action of antidepressants is of the order of 3 to 6 weeks and side effects are often significant.
Nevertheless, while these antidepressants improve the state of the patients in approximately 70% of the cases, they bring about a complete remission of the disease only in 30 to 40% of the patients.
In addition, nearly one-third of the treated subjects are resistant to existing treatments.
Although serotonin, noradrenaline and dopamine are definitely implicated, it is now accepted that changes in monoamine levels produced by AD and the adaptive processes that result from them, in particular the alteration of the sensitivity of some of their receptors, cannot explain by themselves the mechanism of action of antidepressants.
Thus, it is difficult to correlate the time of 3 to 6 weeks required to obtain AD with the increase in synaptic levels of monoamines, which occurs as soon as the first administration of the product.
However, the functional importance of these neoformed neurons in the pathophysiology of mood disorders remains controversial [14].
All these indications demonstrate that depression is a complex disease whose pathophysiology is multifactorial and, consequently, the treatment of such a pathology remains a challenge.
AD drugs used today produce a range of side effects, including dry mouth, blurred vision, and impaired bowel function (diarrhea or constipation).
Although many side effects are transient (such as nausea), some appear to be consistent over time (such as sexual effects) and may affect long-term treatment adherence.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Peptides derived from the propeptide ntsr3 and their use in the treatment of depression
  • Peptides derived from the propeptide ntsr3 and their use in the treatment of depression
  • Peptides derived from the propeptide ntsr3 and their use in the treatment of depression

Examples

Experimental program
Comparison scheme
Effect test

example 1

Spadin Analogs on Trek-1 Canal Activity

[0053]All experiments measuring the effect of spadin analogs and spadin were performed on BTC3 or LNCaP or HEK293 cells stably transfected with the TREK-1 channel, hTREK-1 / HEK293. The hTREK-1 / HEK293 cell line was prepared in the laboratory [21]. These cells also express a reporter gene: E-Green Fluorescent Protein (EGFP) enabling their visualization.

[0054]These cells were seeded at a density of 20,000 cells / dish of 35 mm diameter, 24 to 72 hours before being used.

[0055]The HEK293 cells were cultured in DMEM medium (Gibco) / 10% fetal calf serum (FCS, ICN) / 1% streptomycin+penicillin mixture (Gibco) / 1% glutamax (Gibco) / 0.5 mg / mL of G418 (Sigma) at 37° C. in the presence of 5% CO2.

[0056]The BTC3 cells were cultured in RPMI 1640 medium supplemented with 2.5% FCS, 50 μM beta-mercaptoethanol, 10 mM HEPES, 1 mM sodium pyruvate, 50 μg / ml gentamycin and 1% of a streptomycin+penicillin mixture, at 37° C. in the presence of 5% CO2.

[0057]The LNCaP cells were...

example 2

Tests

Forced Swimming Test (FST)

[0069]A classical behavioral test was developed to determine the antidepressant activity of a substance (Nestier E. J. et al, 2002, Cryan J. and Holmes A., 2005) [22, 23]. The effects of the spadin analogs of the present invention were compared to those of the saline solution in which the spadin analogs of the invention were dissolved and those of the spadin. The mouse strain used was strain C57BI / 6J.

[0070]Saline solution, spadin analogs and spadin were administered to the mice intraperitoneally (IP) at a dose of 100 μg / kg in a bolus of 100 μl.

[0071]The experiment consisted in immersing the mouse for 6 minutes in a container of 15 cm in diameter, 30 cm high containing 11 cm of water at 22° C., 30 minutes after administration of the substance to be tested, and measuring the time of immobility during the last 4 minutes.

[0072]The results of these tests are shown in FIG. 3: FST tests, the values±SEM were statistically compared with the control condition (p...

example 3

Spadine Analogs on Neurogenesis and Synaptogenesis

Study of Neurogenesis by Immunohistochemistry

[0080]BrdU (5-bromo-2′-deoxyuridine) is a synthetic nucleoside analogous to thymidine. It is incorporated during DNA replication during phase S (chromosome duplication during interphase) of the cells in division. Injection of BrdU makes it possible to visualize the cells in division of the tissue to be analyzed by means of immunological labeling. In the present study, the hippocampal neurogenesis of the mice was evaluated after treatment with spadin or the different analogs.

[0081]After the behavioral tests, the mice were injected with BrdU at a final concentration of 120 mg / kg intraperitoneally, according to the modified protocol of Beauquis, J. et al. (Eur J Neurosci 23, 1539-1546, 2007) [25]. Four injections of 300 μl at 10 mg / ml, spaced two hours apart, were performed. 24 hours after the injections, the mice were anesthetized with pentobarbital (100 mg / kg) and then perfused intracardial...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
timeaaaaaaaaaa
diameteraaaaaaaaaa
temperatureaaaaaaaaaa
Login to view more

Abstract

Disclosed are new peptides derived from the neurotensin receptor 3 (NTSR3), and to their use, particularly in the treatment of various diseases, especially depression.

Description

TECHNICAL FIELD OF THE INVENTION[0001]The present invention relates to short peptides derived from the neurotensin receptor 3 (NTSR3) and to their use in various pathologies (diabetes, cancer, epilepsy, stroke, neurodegenerative pathologies, etc.), notably in the treatment of psychiatric pathologies, especially patients suffering from, or having suffered from, depression, for example after a stroke. The present invention relates, in particular, to the use of these peptides for the manufacture of a medicament, for example an antidepressant.[0002]The present invention finds application in the sectors of the pharmaceutical industry and, in particular, in the fields of development of medicaments used in the treatment of various pathologies (diabetes, cancer, stroke, epilepsy, psychiatric diseases, neurodegenerative pathologies, etc.).[0003]In the description below, references in brackets ([ ]) refer to the list of references displayed at the end of the text.PRIOR ART[0004]Depression is ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(United States)
IPC IPC(8): C07K7/06C07K7/08A61K38/08A61K31/7105A61P25/24A61P25/08A61P35/00A61P3/10C12N15/63
CPCC07K7/06C07K7/08A61K38/08A61K31/7105C12N15/63A61P25/08A61P35/00A61P3/10A61P25/24C07K14/723A61K38/00A61K38/10A61P25/00A61P43/00A61P9/10C07K16/00C07K14/435C07K16/18G01N33/54306
Inventor MAZELLA, JEANBORSOTTO, MARCHEURTEAUX, CATHERINEDJILLANI, ALAEDDINEMORENO, SÉBASTIENPIETRI, MARIEL
Owner CENT NAT DE LA RECHERCHE SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap