Particles containing organic catalytic materials and uses
a technology of organic catalytic materials and particles, applied in biochemistry apparatus and processes, inorganic carriers, enzymes, etc., to achieve the effects of simple filtration, easy isolation of reaction products from organic catalytic materials, and easy recovery and reus
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Examples
invention example 1
Preparation of Slurry of Semi-Permeable Particles Using Polycaprolactone and Glucose Oxidase
[0140]In this example the weight ratio of the water-insoluble semi-permeable polymer to the aqueous solution of the organic catalytic material is 0.26. A first water phase (W1) was prepared using 40.3 g of a 3 weight % solution of a carboxy methyl cellulose, sodium salt solution (250,000) in water along with 21.4 g of a 0.015 weight % glucose oxidase solution in 50 mmolar (pH 6.0) phosphate buffer. The oil phase (O) was prepared using 36.5 g of a 35.5 weight % solution of poly(caprolactone) in ethyl acetate, 12.1 g of a 23.5 weight % solution of poly(ethylene oxide-co-b-caprolactone) in ethyl acetate and 153.0 g of ethyl acetate. To this oil phase was added the W1 phase followed by mixing using a Silverson L4R Mixer fitted with a small holed disintegrating head. The resulting water-in-oil (W1 / O) emulsion was further homogenized by using a Microfluidizer model 110T from Microfluidics at a pres...
invention example 2
Preparation of Slurry of Semi-Permeable Particles Using Polycaprolactone and Glucose Oxidase
[0142]In this example the weight ratio of the water-insoluble semi-permeable polymer to the aqueous solution of the organic catalytic material is 0.51. The procedure of Invention Example 1 was followed except that 21.4 g of a 0.015 weight % glucose oxidase solution in 50 mmolar (pH 6.0) phosphate buffer was used in W1 aqueous phase. The oil phase was made using 81.1 g of a 35.5 weight % solution of polycaprolactone in ethyl acetate, 12.1 g of a 23.5 weight % solution of poly(ethylene oxide-co-b-caprolactone) in ethyl acetate, and 108.4 g of ethyl acetate. The amount of enzyme encapsulated in the resulting 3-8 micron semi-permeable particles was found to be 95 μg / g (dry basis), as determined by assay of the W2 aqueous phase (typical procedure described below in Invention example 27).
invention example 3
Preparation of Slurry of Semi-Permeable Particles Using Polycaprolactone and Glucose Oxidase
[0143]In this example the weight ratio of the water-insoluble semi-permeable polymer to the aqueous solution of the organic catalytic material is 0.64. The procedure of Invention Example 1 was followed except that 21.4 g of a 0.019 weight % glucose oxidase solution in 50 mmolar (pH 6.0) phosphate buffer was used in W1 aqueous phase. The oil phase was made using 103.4 g of a 35.5 weight % solution of poly(caprolactone) in ethyl acetate, 12.1 g of a 23.5 weight % solution of poly(ethylene oxide-co-b-caprolactone) in ethyl acetate, and 86.1 g of ethyl acetate. The amount of enzyme encapsulated in the resulting 3-8 μm semi-permeable particles was found to be 96 μg / g (dry basis), as determined by assay of the W2 aqueous phase (typical procedure described below in Invention Example 27).
PUM
Property | Measurement | Unit |
---|---|---|
particle size | aaaaa | aaaaa |
weight ratio | aaaaa | aaaaa |
weight ratio | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com