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Microencapsulated cell of human tissue kallikrein, microencapsulating method and application thereof

A technology of kallikrein and microencapsulation, applied in biochemical equipment and methods, cells modified by introducing foreign genetic material, application, etc., can solve the problem of low content of kallikrein in human tissue, complicated extraction and purification process, It is difficult to meet the clinical needs and other problems, and achieve the effect of obvious application effect and overcoming immune rejection

Inactive Publication Date: 2007-09-19
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of human tissue kallikrein is low in human body fluid, the extraction and purification process is complicated, and it is difficult to meet the clinical needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Construction of tissue kallikrein adenovirus expression vector

[0026] The construction of tissue kallikrein adenovirus expression vector was as described in the literature (document 6: Chao J, Jin L, Chen LM, Chen VC, Chao L. Systemic and portal vein delivery of human tissue kallikrein gene reduces blood pressure in hypertensive rats, Hum Gene Ther., 1996;7:901-911). The tissue kallikrein cDNA fragment is located under the control of the cytomegalovirus (CMV) enhancer / promoter, the cDNA end is added with the bovine growth hormone gene plus polyA signal sequence, and the transcription unit CMV-cHK-pA is inserted into the adenovirus shuttle vector pAdLink.l At the EcoR V site, construct the recombinant plasmid pAd.CMV-cHK. The purified plasmid pAd.CMV-cHK was sent to the Institute of Human Gene Therapy to produce adenovirus Ad.CMV-cHK containing the transcription unit CMV-cHK-pA.

[0027] 2) Adenovirus Ad.CMV-cHK transfected cells

[0028] The recombinant adenovirus...

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PUM

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Abstract

The present invention relates to human tissue kallikrein, and is especially the microcapsulized cell of human tissue kallikrein and its microcapsulizing method and application. On the cell of expressing human tissue kallikrein for gene modification, rigid envelope material with bi-directional flowability is coated. The human tissue kallikrein is suitable for application in treating hypertension and kidney diseases. The present invention has the obvious advantages of adaptability to practical clinical application, obvious application effect and feasible method.

Description

technical field [0001] The present invention relates to human tissue kallikrein, in particular to microencapsulated cells of human tissue kallikrein and its microencapsulation method and application. Background technique [0002] Tissue kallikrein is a member of the serine protease family, which can cleave low-molecular-weight kininogen to produce biologically active kinin (document 1: Clements J.A. The glandular kallikrein family of enzymes: tissue-specific expression and hormonal regulation, Endocrine Rev. , 1989; 10:393-419.; Literature 2: Bhoola K.D., C.D.Figueroa, K.Worthy, Bioregulation of kinins: kallikreins, kininogens, and kininases, Pharmacol.Rev., 1992; 44:1-8); B 2 Receptor binding can trigger a series of biological activities, such as smooth muscle contraction and relaxation, electrolyte balance, glucose transport, pain generation, inflammation formation, increased vascular permeability, blood pressure drop, etc. (Document 2: Bhoola K.D., C.D.Figueroa, K . Wor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00C12N5/10C12N9/50C12N15/57C12N15/85C12N15/861C12N11/02A61K38/48A61K35/12A61K9/48A61P9/12A61P13/12
Inventor 张玉奎于晓明张维冰杨青徐茂兰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI