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Amido glycoside of sea pearl oyster, prepartion method, and usage

A technology of aminoglycosides and amino acids, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, glycopeptide components, etc., and can solve problems such as the preparation of aminoglycosides

Active Publication Date: 2007-11-21
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no report of making aminoglycosides from marine pearl oysters

Method used

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  • Amido glycoside of sea pearl oyster, prepartion method, and usage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] After pulverizing 1000g of marine pearl oyster, use 22KHz ultrasonic waves to break the cell membrane, add 2000mL of distilled water and mix evenly, use 1mol / L fluoxetine to adjust the pH value to 6.0, and then obtain the acid solution, take 1000mL of the acid solution and add 2.8g of papain, and then The pH adjusted with 0.1 mol / L NaOH solution was 7.5, the enzymolysis temperature was 50° C., and the enzymolysis time was 6 hours to obtain an enzymolysis solution. Take 30g trypsin, 0.025mol, potassium dihydrogen phosphate, 0.01mol potassium chloride, 4mmol sodium taurocholate and 1mmol phosphatidic acid choline, dissolve with a small amount of water, add the enzymolysis solution obtained above, mix well and set The volume was increased to 1000mL, the pH value of the solution was adjusted to 8.0 with 0.1mol / L NaOH solution, the enzymolysis temperature was 45°C, the enzymolysis time was 2h, and the soluble powder was obtained by freeze-drying.

[0027] Dissolve the powder...

Embodiment 2

[0029] After pulverizing 1000g of marine pearl oyster, use 24KHz ultrasonic waves to break the cell membrane, add 2000mL of distilled water and mix evenly, use 1mol / L lactic acid to adjust the pH value to 6.5, and then obtain the acid solution. Take 1000mL of the acid solution and add 3.5g of papain, and then use The pH adjusted by the mol / L NaOH solution was 7.0, the enzymolysis temperature was 45° C., and the enzymolysis time was 8 hours to obtain an enzymolysis solution. Take 50g of trypsin, 0.032mol of potassium dihydrogen phosphate, 0.03mol of potassium chloride, 6mmol of sodium taurocholate and 2mmol of phosphatidic acid choline, dissolve them in a small amount of water, add the enzymatic hydrolysis solution obtained above, mix well and constant volume to 1000mL, adjust the pH value of the solution to 8.5 with 0.1mol / L NaOH solution, enzymolysis temperature 50°C, enzymolysis time 4h, freeze-dry to obtain soluble powder.

[0030] Add pure ethanol to the powder obtained ab...

Embodiment 3

[0031] Embodiment 3: the effect of sea pearl oyster aminoglycoside against herpes simplex virus type I (HSV-I)

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Abstract

This invention relates to a method for preparing an aminoglycoside and its application. The aminoglycoside has a relative molecular weight range within 1300-33000, and is composed of repeated amino acid fragments (-GASRERCAKLSG-) and chondroitin sulfate B, wherein chondroitin sulfate B is bonded with glutamic acid residue of the oligopeptide. The aminoglycoside is prepared oceanic pearl by breaking cell membrane with ultrasonic wave or liquid nitrogen, acidolyzing, enzymolyzing in gastric juice and intestinal juice-like conditions, extracting with solvent, and centrifuging for separation. The obtained aminoglycoside has antiviral and antiatherosclerotic functions, thus can be used in antiviral drugs, antiatherosclerotic drugs, eye drops, soluble pearl powder, cosmetics, functional foods and healthcare products.

Description

technical field [0001] The present invention relates to a new aminoglycoside, in particular to an aminoglycoside obtained from marine pearl oysters, and to a preparation method and application of the aminoglycoside. Background technique [0002] Aminoglycosides are a class of compounds composed of amino acid molecules and glycogen moieties. Aminoglycosides are widely used in antibiotics, antibiotics, anticancer drugs, and health products. So far, people mainly use animals, plants, fungi, and bacteria as raw materials, and use specific enzymes to hydrolyze the raw materials to obtain aminoglycosides. But so far there is no report on the preparation of aminoglycosides from marine pearl oysters. Contents of the invention [0003] The object of the present invention is to obtain a new aminoglycoside from marine pearl oyster. Another object is to develop a process for the preparation of such aminoglycosides. A further object is to develop applications of such aminoglycoside...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K9/00C07K14/435A61K38/14A61K38/16A61P31/12A61P9/10
Inventor 张偲尹浩龙丽娟闻克威张士忠王世全王士奎
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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