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Apocynum extract and extracting method thereof

An apocynum extract and an extraction method technology are applied to the extraction of flavonoids and the field of flavonoids, which can solve the problems of inability to obtain exact characterization and high ointment yield, achieve good drug development prospects, easy to take and low toxicity Effect

Inactive Publication Date: 2007-12-05
李青山
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The present invention aims at the problem that the high-purity extract of Apocynum apocynum can not be obtained at present, so the problem that Apocynum apocynum is used as crude drug or crude extract is used as medicine, and provides a kind of Apocynum apocynum (high purity) extract with definite characterization and outstanding effect in medicine
[0009] The present invention also provides a high-purity extraction method for Apocynum apocynum extract aiming at the problems that the existing extraction method of Apocynum apocynum extract has a high paste yield and the obtained extracts are all crude extracts

Method used

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  • Apocynum extract and extracting method thereof
  • Apocynum extract and extracting method thereof

Examples

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Effect test

Embodiment 1

[0046] 1. Preparation of high-purity extract of Apocynum apocynum

[0047]Get 300g of Apocynum basil leaves (baked at 200°C for 1 hour), at a temperature of 85°C-100°C, reflux extract with 3000mL of 40% ethanol water for 3 times (3000mL×3), each time for 0.5hr, combine the extracts, Stand overnight at room temperature, filter, remove precipitation, concentrate the filtrate under reduced pressure (60°C, 0.08-0.1MPa), to a total volume of 900mL, add 180mL of 1% chitosan acetic acid solution to the concentrate (equivalent to 1.8 grams of chitosan , can be made into chitosan solutions of other concentrations, as long as the chitosan is fully dissolved), stirred for 2 minutes, left at room temperature for 2-4 hours, filtered with suction, discarded the residue, and the supernatant was treated with polyamide resin Column (column volume is about 300mL, flow rate 300mL / hr), the effluent is detected by FeCl3 reaction to confirm whether the resin is saturated; then use 600mL water, adju...

Embodiment 2

[0061] 1. Preparation of high-purity extract of Apocynum apocynum

[0062] Take 300g of dried white hemp leaves, and extract 3 times with 60% ethanol water under reflux (3750, 2700mL, 2400) at a temperature of 85°C-100°C, each time for 1hr, combine the extracts, and let stand overnight at room temperature, Filtrate, remove the precipitate, concentrate the filtrate under reduced pressure (60°C, 0.08-0.1MPa) to a total volume of 900mL, add 180mL of 1% chitosan acetic acid solution to the concentrated solution, then add 180mL of 1% gelatin solution and stir for 2min at room temperature Stand still for 2-4 hours, filter with suction, discard the residue, pass the supernatant through a treated polyamide resin column (column volume about 300mL, flow rate 300mL / hr), and detect the effluent by FeCl3 reaction to confirm whether the resin is saturated; Carry out elution with the ethanol of 600mL10% subsequently, eluent abandons, then with 60% ethanol and acetone aqueous elution, elution...

Embodiment 3

[0076] 1. Preparation of high-purity extract of Apocynum apocynum

[0077] Take 300g of dried Apocynum venetum leaves, extract 3 times (3000mL, 2700mL, 2400mL) with 45% ethanol water under reflux at a temperature of 85°C-100°C, 1hr each time, combine the extracts, let stand overnight at room temperature, filter , to remove precipitation, and concentrate the filtrate under reduced pressure (60°C, 0.08-0.1MPa) to a total volume of 900mL, add 160mL of 1% ZTC solution component A to the concentrated solution, and then add 160mL of 1% ZTC solution B component and stir for 2min at room temperature Stand still for 2-4 hours, filter with suction, discard the residue, pass the supernatant through a treated polyamide resin column (column volume about 300mL, flow rate 300mL / hr), and detect the effluent by FeCl3 reaction to confirm whether the resin is saturated; Then eluted with 900mL water, discarded the eluent, then eluted with 85% aqueous methanol, eluted to no AlCl3 reaction, the elu...

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Abstract

The invention relates toa an Apocynum extract and extracting method, which consists of, (1) determining the extract with hyperin as the representing composition, the content of flavones in the extract is 35-90%, (2) after the complete hydrolysis of the extract, the content of meletin compound is 30-80%, (3) the content of hyperin in the extract is 15-55%.

Description

technical field [0001] The invention relates to an extract of apocynum apocynum and an extraction method thereof, in particular to a flavonoid extracted from apocynum apocynum and an extraction method of the flavonoid. Background technique [0002] Apocynum leaf is a traditional medicine for calming the liver and calming the wind. It has the functions of calming the liver and calming the nerves, clearing heat and diuresis. It is used for liver yang dizziness, heart-reducing insomnia, edema and oliguria; hypertension, neurasthenia, nephritis and edema. Modern pharmacological studies have found that it also has a wide range of pharmacological effects such as lowering blood fat, anti-atherosclerosis, anti-depression and liver protection. Its active ingredients are flavonoids. But at present the clinical medicine that takes Apocynum apocynum as raw material is all crude extract product or health care product as: " Apocynum apocynum leaf " (Ministry of Health drug standard Chine...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/24A61P25/24A61P3/06A61P9/10
Inventor 李青山侯晋军韩利文韩玲革
Owner 李青山
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