Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

1-amino cyclopropane-1carboxylic oxidase and its coding gene and application

An aminocyclopropane, encoding gene technology, applied in oxidoreductase, application, genetic engineering and other directions, can solve the problems of cotton fiber development and elongation regulation complexity and other issues

Inactive Publication Date: 2008-06-11
PEKING UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Scientists around the world have done a lot of in-depth and meticulous work on the ACO gene, but due to the multifunctionality of ethylene hormones, the complexity of cotton fiber development and elongation regulation, there are still a lot of unknown areas to be studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • 1-amino cyclopropane-1carboxylic oxidase and its coding gene and application
  • 1-amino cyclopropane-1carboxylic oxidase and its coding gene and application
  • 1-amino cyclopropane-1carboxylic oxidase and its coding gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1. Analysis of hybridization results of cotton genome cDNA chip and acquisition of ethylene synthase gene Gh-ACO1 and its coding gene

[0037] The wild-type and mutant cotton materials at different development stages were selected, and the cDNA chip containing 11,692 independent cotton genes (article number: CAP-F0003) manufactured by Beijing Boao Biochip Co., Ltd. was used to screen for highly expressed genes during fiber development.

[0038] The specific operation steps of this experiment are:

[0039] 1. RNA extraction, reverse transcription, labeling and hybridization experiments. Ovules from wild-type upland cotton (wt) 3 days before flowering, on the day of flowering (0 day) and 3 days after flowering, ovules and fibers mixed at 5 days, 10 days, 15 days and 20 days after flowering, and without Total RNA was extracted from the ovules of the hairy mutant (the hairless mutant was purchased from the Institute of Botany, Chinese Academy of Sciences) 3 days an...

Embodiment 2

[0052] Example 2. Relationship between Gh-ACO1 mRNA expression level and cotton fiber elongation

[0053] The wild-type upland cotton (wt) and hairless mutant (fl mutant) cotton materials at different stages of development were selected, and the expression level of Gh-ACO1 in these materials was analyzed by real-time quantitative PCR (quantitation PCR), so as to obtain the expression level of Gh-ACO1 relationship to fiber elongation.

[0054] The specific operation steps of this experiment are:

[0055] 1. Extraction of RNA. According to the operating manual Micro-TO-Midi Total RNA Purification System (Invitrogen, USA) from wild-type cotton on the day of flowering and ovules 3 days after flowering, a mixture of ovules and fibers 5 days after flowering, 10 days, 15 days and 20 days after flowering Total RNA was extracted from the fibers of the day 1 and the ovules of the mutant 10 days after anthesis.

[0056] 2. Preparation of cDNA template. 5 μg of total RNA was used as a...

Embodiment 3

[0066] Example 3. Analysis of the expression level of Gh-ACO1 mRNA in different tissues

[0067] Wild-type upland cotton (wt) and hairless mutant (flmutant) cotton materials at different stages of development were selected, and real-time quantitative PCR (quantitation PCR) was used to analyze the expression of Gh-ACO1 in these materials, so as to obtain the expression level of Gh-ACO1 and Fiber elongation relationship.

[0068] The specific operation steps of this experiment are:

[0069] 1. Extraction of RNA. Cotton materials were collected, including roots, stems, leaves, fibers and ovules 10 days after flowering of wild-type cotton, and ovules 10 days after flowering of mutants according to the operating manual Micro-TO-Midi Total RNA Purification System (Invitrogen, USA). Total RNA was extracted separately.

[0070] 2. Preparation of cDNA template. Use 5ug of total RNA as a template, digest with DNase I (Invitrogen, USA) to prevent contamination of genomic DNA, and then ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a species 1-amino cyclopropane-1-carboxylic acids oxidase and its coding gene and application. It is one of the following amino acid sequence protein: the second sequence amino acid residue. And one or many amino acid residues of the second sequence amino acid residue sequence are replaced / deleted / added and reacted with ethylene to compose protein. It can be used to culture plant species with fiber length increasing.

Description

technical field [0001] The present invention relates to class 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase) and its encoding gene and application, in particular to class 1-aminocyclopropane-1-carboxylate oxidase (ACC oxidase) and its encoding gene Application in cotton fiber production. Background technique [0002] Cotton is one of the most important economic crops in the world. It is the primary fiber crop and important oil crop. All major cotton-producing countries attach great importance to the research on cotton development and genetics. The development of cotton fiber appears in the form of single cells, with large morphological changes, and its length / diameter can reach 1000-3000; the secondary cell wall of mature cotton fiber is almost composed of pure cellulose. Therefore, cotton fiber is not only a study of carbon participating in cellulose Synthesis is also an ideal material for studying cell elongation (because complex cell division and interference wi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02C12N15/53C12N15/63C12N15/82
Inventor 朱玉贤施永辉卢迎春朱生伟姬生健
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com