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Skin wound repairing agar/collagen dressing and its prepn and application

A skin trauma and collagen technology, applied in the field of biomedical materials, can solve the problems of not using agar, and achieve the effects of accelerating tissue formation, increasing added value, and convenient operation

Inactive Publication Date: 2008-07-16
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Therefore, the materials used in the existing skin repair technology do not use agar and its derivatives. In view of the gel-forming properties of agar, some patents only involve agar as a cooling, thickening dressing or as a cell encapsulation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Porous Composite Scaffold Preparation and Skin Dressing

[0029] (1) Agar is dissolved in hot water at 85-95°C, the mass concentration percentage is 1%, collagen (or gelatin) is dissolved in 0.04-0.06M acetic acid solution (or swelled in water), and the mass concentration percentage is 0.5%- 0.6%.

[0030] (2) Mix the agar and collagen (or gelatin) solution after step (1) in a mass ratio of 1: 2, stir for 10 minutes under water bath conditions (water bath temperature 70° C.), centrifugal degassing (500~1000 rpm Divide, 2-5 minutes), then pour into the mold to form (make the height of the mixture in the mold be 5-6mm), put the mold into the refrigerator (-18°C) and freeze for 2-4 hours.

[0031] (3) Put the mold into a freeze dryer, freeze at -35~-45°C for 1~3 hours, then pump air at 0°C for 17~24 hours, freeze-dry to form a porous composite scaffold with a thickness of about 3~4mm, Glutaraldehyde (mass concentration: 0.5%) was cross-linked for half an hour, ...

Embodiment 2

[0033] Embodiment 2 composite film preparation and skin dressing

[0034] (1) Agar is dissolved in hot water at 85-95°C, the mass concentration percentage is 1%, gelatin (or collagen) is dissolved in 0.04-0.06M acetic acid solution (or swelled in water), and the mass concentration percentage is 0.5%- 0.6%.

[0035] (2) Mix the agar and gelatin (or collagen) solution after step (1) in a mass ratio of 1: 4, stir for 3 minutes under water bath conditions (water bath temperature 70° C.), centrifugal degassing (500~1000 rpm minutes, 2-5 minutes), and then poured into a mold for molding (make the height of the mixture in the mold be 3-4mm).

[0036] (3) put it into a blast dryer, and dry at 50-55°C for 48-72 hours to obtain a composite film with a film thickness of about 2 mm. Sterilized with ethylene oxide.

[0037] The composite membrane material is prepared in the form of a disc with a diameter of 1.5 cm and a thickness of 2 to 3 mm, cross-linked with glutaraldehyde (0.5% in mas...

Embodiment 3

[0038] Example 3 Subcutaneous fat tissue engineering scaffold

[0039] (1) Agar is dissolved in hot water at 85-95°C, the mass concentration percentage is 1%, collagen (or gelatin) is swollen in water (or 0.04-0.06M acetic acid solution), and the mass concentration percentage is 0.5%- 0.6%.

[0040] (2) Mix the agar and collagen (or gelatin) solution after step (1) in a mass ratio of 2: 1, stir under water bath conditions for 20 minutes (water bath temperature 60° C.), centrifugal degassing (500~1000 rpm 2-5 minutes), then pour it into a mold to form (make the height of the mixture in the mold 5-6mm), put it in the refrigerator (-18°C) and freeze it for 2-4 hours.

[0041] (3) Put it into a freeze dryer, freeze at -40°C for 2 hours, then pump air at 0°C for 20 hours, and freeze-dry to form a porous composite scaffold with a thickness of about 3-4mm.

[0042] (4) Fumigate the porous composite scaffold on a 2% glutaraldehyde aqueous solution for half an hour for cross-linking ...

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Abstract

The present invention provides one kind of skin wound repairing agar / collagen dressing and its preparation process and application. Agar and collagen or gelatin are mixed, and the mixture is freeze dried to obtain porous compound rack or compound film for promoting the repair of skin wound, and the rack may be also used in other soft tissue engineering. The porous compound rack and compound film of present invention has simple operation, low cost and wide use, and may be used in repairing burnt and ulcer skin as well as for cartilage, cornea, fat and soft tissue engineering.

Description

technical field [0001] The invention relates to biomedical material technology, in particular to an agar / collagen skin wound repair dressing and its preparation method and application. Background technique [0002] Skin diseases caused by extensive burns and ulcers are common clinical diseases, and skin reconstruction or transplantation is one of the important measures to save patients' lives. Since the amount of autologous skin available for transplantation is not enough to solve large-scale repairs, and there are problems such as immune reactions in allogeneic or heterogeneous skin, various tissue-engineered skin or biomaterial dressings have become the way to solve the problem. [0003] The application of skin dressings began in BC. Plant fiber plants such as cotton fibers were used as dressings for skin wounds, which played a good role in protecting wounds and promoting healing for small wounds. The real research at home and abroad began at the beginning of the last cen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/32
Inventor 汤顺清毛萱
Owner JINAN UNIVERSITY
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