Method for biological catalysis for preparing konjak glucomannan esters in mixed solvent

The technology of konjac glucomannan and glucomannan ester is applied in the field of preparation of functional polymer materials, and can solve the problems of easy generation of by-products, poor reaction selectivity, complicated reaction system, etc., and achieves simple and easily controllable reaction process, The effect of mild reaction conditions and high degree of reaction

Inactive Publication Date: 2008-10-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this method prepares glucomannan ester, and reaction system is complicated, and reaction temperature is higher, and energy consumption is big, and reaction selectivity is poor, easily produces by-product and product is difficult to separate; And can bring more serious environmental pollution problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The water activity is 0.5g konjac glucomannan of 0.11, 100U is derived from the lipase of Candida antarctica, 5.0ml vinyl acetate, 5.0ml mixed solvent (wherein mixed solvent, main solvent isooctane and cosolvent N, N-dimethylacetamide (volume ratio: 9:1) was put into a stoppered Erlenmeyer flask, the bottle mouth was strictly sealed with polytetrafluoroethylene sealing tape, and then placed in a water bath constant temperature oscillator at 30°C and 100rpm to oscillate, and reacted for 6 hours. , take out the reactor, add absolute ethanol to stop the reaction, remove the precipitate obtained by suction filtration to remove the mixed solvent, wash thoroughly with absolute ethanol, and dry to constant weight. The lipase is removed through an 80-mesh sieve to obtain konjac glucomannan acetate with a degree of substitution of 0.13.

Embodiment 2

[0022] The water activity is 0.5g konjac glucomannan of 0.53, 1000U is derived from the lipase of Candida antarctica and 0.9ml vinyl acetate, 20.0ml mixed solvent (wherein mixed solvent, main solvent carbon tetrachloride and auxiliary solvent N , the volume ratio of N dimethylacetamide is 9:1) into a stoppered Erlenmeyer flask, tightly sealed with polytetrafluoroethylene sealing tape, and then placed in a water bath constant temperature oscillator at 70°C and 200rpm to vibrate, and the reaction After 48 hours, the reactor was taken out, and anhydrous ethanol was added to stop the reaction. The precipitate obtained by removing the mixed solvent was suction-filtered, washed thoroughly with anhydrous ethanol, and dried to constant weight. The lipase is removed through a 200-mesh sieve to obtain konjac glucomannan acetate with a degree of substitution of 0.15.

Embodiment 3

[0024] The water activity is 0.5g konjac glucomannan of 0.23, 1000U lipase derived from Candida antarctica and 5.0ml vinyl acetate, 50.0ml mixed solvent (wherein mixed solvent, main solvent isooctane and cosolvent N, The volume ratio of N-dimethylacetamide is 9:1) into a stoppered Erlenmeyer flask, the bottle mouth is strictly sealed with a polytetrafluoroethylene sealing tape, and then placed in a water bath constant temperature oscillator at 50°C and 200rpm to oscillate and react for 24 hours Finally, the reactor was taken out, and absolute ethanol was added to stop the reaction, the precipitate obtained by removing the mixed solvent by suction filtration was fully washed with absolute ethanol, and then dried to constant weight. Remove the lipase through a 200-mesh sieve to obtain konjac glucomannan acetate with a degree of substitution of 0.28.

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PUM

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Abstract

The invention discloses a method for preparing konjak gluglucosan in mixed organic solvent with biological catalysis. It takes a mixed solvent system comprising main solvent of hydrophobic and adjuvant solvent of hydrophilic as reaction medium, taking aliphatic acid ethyenyl ester with carbon chain length being C2-C18 as acyl donor, and proceeding esterification reaction by using lipase and getting final product. The invention is characterized by soft reaction condition, low energy consumption, environment-friendly, simple process, selective and controllable reaction and easy-to-separate product.

Description

technical field [0001] The invention relates to a preparation method of a functional polymer material, in particular to a method for biocatalyzing the preparation of konjac glucomannan ester in a mixed solvent system. Background technique [0002] Konjac Glucomannan (KGM for short) is a polysaccharide from the plant Konjac, and it is also a unique renewable resource in southern my country and Southeast Asian countries. KGM is a complex polysaccharide composed of D-glucose (G) and D-mannose (M) at a molar ratio of 1:1.6 or 1:1.69 through β-1,4-pyranoside bonds, usually with a short Branched and contains a small amount of acetyl groups. KGM has high viscosity and unique rheological properties, excellent biodegradability, biocompatibility and many unique physiological and pharmacological functions, and has important clinical application value in the field of medicine and preventive medicine. At present, it has been widely used in food, chemical, textile, petroleum and cosmeti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/08C08B37/02C12N9/00C12S3/10
Inventor 李光吉廖启金宗敏华
Owner SOUTH CHINA UNIV OF TECH
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