Method for gel electrophoresis separation of serum lipoprotein and quantization detection thereof

Abstract

The invention discloses a method for gel electrophoresis separation of serum, wherein sectionalized polyacrylamide gels with three different concentrations are used as electrophoresis carrier for forming temperature gradient in the electrophoresis tank, so as to achieve synchronous electrophoretic separation for serum lipoprotein and its subcomponents, wherein the concentrations of the polyacrylamide gel being separation gel 1 3.0%, separation gel 2 3.6%, separation gel 3 7.0%.

Description

technical field [0001] The invention discloses a new method for gel electrophoresis separation of serum lipoproteins and its quantitative detection, relates to a practical method for effectively expressing the metabolic equilibrium state of serum lipoproteins, and is widely used in medical detection and research. Background technique [0002] Serum lipoprotein has a special particle structure, and its composition is complex and heterogeneous. During the metabolic process, it continuously evolves and exchanges substances in sequence, and is usually in a coordinated dynamic equilibrium state. Dyslipoproteinemia is closely related to cardiovascular and cerebrovascular diseases, and it is very important in clinical practice. Over the years, the methods for detecting serum lipoproteins have been continuously improved, such as gradient gel electrophoresis, but there has been a lack of practical methods to effectively express the metabolic balance of serum lipoproteins in clinical ...

AI Technical Summary

Problems solved by technology

There are three main problems: 1. Due to the great difference in the particle characteristics of different types of serum lipoproteins, it is difficult to take into account the experimental conditions of a single method, and the ability to express them simultaneously is weak. Usually, separate methods are used for detection

2. Due to the inconsistency of the reference system in the analysis process, the results of separate determinations cannot be placed in a unified whole of serum lipoproteins to compare their interrelationships

3. Due to the high requirements of experimental conditions, such as a stable linear concentration gradient, it is difficult to meet the needs of clinical testing

The 2001 survey found that 94.9% of patients with coronary heart disease and hyperlipidemia in my country failed to obtain ideal blood lipid control

Therefore, theoretically, it is difficult to explain the pathogenesis of patients from isolated blood lipid factors

There are some problems as follows: (1) The integrity of the serum lipoprotein particle structure is ignored, and the serum lipoprotein exerts its physiological function under the premise of maintaining the complete particle structure

(2) Ignoring the integrity of serum lipoprotein metabolism, the composition of serum lipoprotein is complex and uneven, and its metabolism is usually in a coordinated and balanced state

(2) The main problems existing in the previous methodology are: (1) The separation methods are mutually independent, and it is difficult to carry out synchronous separation

Due to the great difference in particle characteristics of different types of serum lipoproteins, it is difficult to set electrophoresis conditions and the simultaneous expression ability is poor

(2) There is no unified reference for the analysis method, and a unified comparison cannot be carried out

Due to the neglect of the experimental principle of "uniqueness of the reference system", the measurement results lack mutual comparability, and the overall status of serum lipoprotein metabolic balance cannot be uniformly evaluated

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