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Multilayer hollow Nano fiber in use for cell culture, and preparation method

A technology for culturing nanofibers and cells, applied in tissue cell/virus culture devices, biochemical equipment and methods, chemical instruments and methods, etc., can solve problems such as multi-layer hollow nanofibers that have not been reported, and achieve free choice of components, The effect of simple preparation device, easy storage and transportation

Inactive Publication Date: 2009-02-25
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, no method for preparing multilayer hollow nanofibers has been reported

Method used

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  • Multilayer hollow Nano fiber in use for cell culture, and preparation method
  • Multilayer hollow Nano fiber in use for cell culture, and preparation method

Examples

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Effect test

preparation example Construction

[0029] The preparation method is to dissolve water-soluble polyelectrolyte: sodium polystyrene sulfonate and polyaminoallyl hydrochloride in water, and then assemble it layer by layer on the surface of nano electrospun fibers. The specific steps are as follows:

[0030] 1) Water is used as a solvent to configure positively or negatively charged polyelectrolyte solutions respectively;

[0031] 2) Dip the electrospun polystyrene (PS) fiber into the oppositely charged polyelectrolyte polyaminoallyl hydrochloride solution, rinse with distilled water several times; then dip into the sodium polystyrene sulfonate solution, Multiple layers of polyelectrolytes were assembled on the fiber surface repeatedly.

[0032] 3) Using an organic solvent tetrahydrofuran to selectively remove the internal polystyrene fiber as a template, while the solvent does not damage the external polyelectrolyte, thereby obtaining a multilayer hollow fiber.

[0033] The above-mentioned organic solvent may als...

Embodiment 1

[0034]Example 1: Preparation of (poly(allylaminehydrochloride) (poly(allylaminehydrochloride) (PAH, polycation) / polystyrenesulfonate, sodium salt) (PSS, polyanion) with a size of about 400nm ) 4 Multilayer hollow fiber:

[0035] Preparation of aqueous solution: Polyaminoallyl hydrochloride and sodium polystyrene sulfonate were respectively dissolved in 0.5M sodium chloride aqueous solution to prepare a polyelectrolyte aqueous solution with a concentration of 1.0 mg / ml.

[0036] Template preparation: Polystyrene nanofibers were prepared with an electrospinning spinneret, with a size distribution of 800-1300nm.

[0037] Immerse the negatively charged polystyrene fiber into the polycationic polyaminoallyl hydrochloride solution, so that the surface of the fiber is adsorbed with a layer of polyaminoallyl hydrochloride, so that the surface is positively charged, and the excess polymer is washed away. things. Then immerse the fiber into the sodium polystyrene sulfonate solution, ...

Embodiment 2

[0038] Embodiment 2: use PS fiber as a template, polyaminoallyl hydrochloride solution concentration is 0.5mg / ml, bovine serum albumin aqueous solution concentration is 1.0mg / ml, operation steps are the same as above, and the available size is 400 nanometers Left and right multilayer polyaminoallyl hydrochloride / BSA hollow nanofibers.

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Abstract

A multi-layer hollow nanofiber used to culture cells is prepared through providing electrically spun fibers as template, alternatively adsorbing the polymers with positive and negative charges onto the surface of template, and using organic solvent to remove said template.

Description

technical field [0001] The invention relates to a method for preparing a multilayer hollow nanofiber that can be used for cell culture. The hollow multilayer nanofiber can be used as a support material for cell culture and belongs to the technical field of cell culture carrier materials. Background technique [0002] The hollow nanofiber bioreactor can be used for both suspension cell culture and adherent cell culture. The principle is: to simulate the three-dimensional state of cell growth in the body, and use the longitudinal arrangement of thousands of hollow fibers in the reactor to provide an in vitro growth microenvironment that approximates physiological conditions for cells, so that cells can continue to grow. Hollow nanofiber is a fine tubular structure, the tube wall is an extremely thin semi-permeable membrane, rich in capillaries, the fiber tube is perfused with oxygen-filled serum-free culture medium during culture, and the outer wall of the tube is for cell adh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D69/08C12M3/06
Inventor 葛丽芹潘超顾忠泽
Owner SOUTHEAST UNIV
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