Extraction method of Chinese medicine of gold buckwheat raw anthocyanin

A technology of proanthocyanidin and extraction method, which is applied in the direction of medical formula, drug combination, medical preparations containing active ingredients, etc., to achieve significant antitussive and expectorant effects and simple process

Inactive Publication Date: 2009-07-08
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the extraction method of proanthocyanidins from the traditional Chinese medicine golden buckwheat and its antitussive and expectorant effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Process and steps in the present embodiment are as follows:

[0016] (1) mechanically pulverizing the traditional Chinese medicine golden buckwheat, and passing the particles through a 50-mesh sieve to obtain golden buckwheat coarse powder;

[0017] (2) Add 800 grams of the above-mentioned golden buckwheat coarse powder into 2000 milliliters of distilled water, stir and extract 3 times; each extraction time is 5 hours, and the extraction temperature is 20° C.;

[0018] (3) Collect and combine the above-mentioned three extracts, filter it to obtain the filtrate, which is the filtered extract; gradually add sodium chloride in the extract, and keep stirring until the saturated sodium chloride is separated out Then filter, and the filtrate is extracted 3 times with the organic solvent ethyl acetate; Add anhydrous sodium sulfate to dry; then filter, and concentrate the obtained filtrate to 1 / 5 of its original volume under reduced pressure;

[0019] (4) Add 3 times the volu...

Embodiment 2

[0021] Process and steps in the present embodiment are as follows:

[0022] (1) mechanically pulverizing the Chinese medicine golden buckwheat, and passing the particles through a 60-mesh sieve to obtain golden buckwheat coarse powder;

[0023] (2) adding 1000 grams of the above-mentioned golden buckwheat coarse powder material to 4000 milliliters of concentration in 20% ethanol aqueous solution, stirring and leaching 3 times; each leaching time is 12 hours, and the leaching temperature is 50 ° C;

[0024] (3) Collect and combine the above-mentioned three extracts, filter it to obtain the filtrate, which is the filtered extract; gradually add sodium chloride in the extract, and keep stirring until the saturated sodium chloride is separated out Filter then, and filtrate is extracted 4 times with organic solvent ethyl acetate; The consumption of ethyl acetate is decided according to the amount of filtrate, adopts the volume ratio of filtrate and ethyl acetate to be 1: 1; Merge t...

Embodiment 3

[0027] Process and steps in the present embodiment are as follows:

[0028] (1) Mechanically pulverizing the Chinese medicine Golden Buckwheat, passing the particles through a 40-mesh sieve to obtain the Golden Buckwheat coarse powder;

[0029] (2) adding 1000 grams of the above-mentioned golden buckwheat coarse powder material to 4000 milliliters of concentration in 10% ethanol aqueous solution, stirring and leaching 2 times; each leaching time is 8 hours, and the leaching temperature is 40 ° C;

[0030] (3) Collect and combine the above-mentioned secondary extracts, filter it to obtain the filtrate, which is the filtered extract; gradually add sodium chloride in the extract, and keep stirring until the sodium chloride is saturated Separate out; Then filter, and the filtrate is extracted 4 times with organic solvent ethyl acetate; The consumption of ethyl acetate is decided according to the amount of filtrate, adopts the volume ratio of filtrate and ethyl acetate to be 1: 1; ...

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Abstract

The invention relates to a process for extracting proanthocyanidins from Chinese herbal medicine golden buckwheat, which belongs to the technical field of Chinese herbal medicine extraction chemistry and chemical industry. The technical process of the method of the present invention is as follows: first, the Chinese herbal medicine golden buckwheat is pulverized and sieved, and the obtained golden buckwheat coarse powder is leached with distilled water or low-concentration ethanol aqueous solution, and the leaching solution is saturated with inorganic salt sodium chloride and filtered, and then used Organic solvent extraction, drying the extracted organic solvent phase with anhydrous sodium sulfate, then filtering, and concentrating the obtained filtrate under reduced pressure; then adding 3 to 6 times the volume of a low-polarity organic solvent to the filtrate concentrate, stirring, and then Stand still for 5 to 12 hours, and wait for its precipitation; the precipitate is obtained after filtration, and the water-soluble proanthocyanidin extract is obtained after drying. The purity of the proanthocyanidin extract is greater than 95%, and the yield is 0.6-0.7%. The golden buckwheat proanthocyanidin extract obtained by the method of the invention has significant antitussive and expectorant effects, and can be used as an active ingredient of antitussive and expectorant drugs.

Description

technical field [0001] The invention relates to a process for extracting proanthocyanidins from Chinese medicine golden buckwheat, which belongs to the technical field of Chinese herbal medicine extraction chemistry and chemical industry. Background technique [0002] Wild golden buckwheat (Fagopyrum dibotrys) is a perennial herbaceous plant of the genus Buckwheat in the Polygonaceae family. It is a resource plant with rich nutrition and important medicinal value. It has been listed as a national second-class protected plant in China. Modern research has confirmed that the main active ingredient contained in the rhizome of golden buckwheat is a mixture of proanthocyanidins, including barley catechin, proanthocyanidin B-2, B-4, and 3,3'-digallate of proanthocyanidin B-2. Proanthocyanidin substances are polymerized by different numbers of flavan-3-ols or flavan-3,4-diols, and 2-4 polymers are called oligomeric proanthocyanidin Oligomers (OPC). [0003] Proanthocyanidin molecu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62C07D311/60C07H17/07A61K31/352A61K31/7048A61P11/10A61P11/14
Inventor 吕敬慈雍克岚陆伟华陈旭
Owner SHANGHAI UNIV
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