Culture in vitro for fragrant fruit tree

Abstract

The invention discloses a method for in vitro cultivation of fragrant fruit trees. The culture method comprises the following steps: 1) induction of embryogenic callus: using sterilized seeds as explants, placing them on an embryogenic callus induction medium to induce embryogenic callus; The embryogenic callus induction medium was added 6-benzyl adenine and 2,4-D on the basis of MS basic medium; 2) Differentiation of somatic embryos: the embryogenic callus was placed in differentiation culture basal induction of differentiation; the differentiation medium is added on the basis of MS basic medium with 6-benzyl adenine and α-naphthalene acetic acid; 3) rooting and plant regeneration: the differentiated cotyledon embryos are placed in rooting culture Rooting is induced on the basis to obtain regenerated plants of the fragrant fruit tree; the rooting medium is 1 / 4-3 / 4MS medium. The invention lays a good foundation for expanding the reproduction coefficient of the fragrant fruit tree, the germplasm preservation and genetic transformation of the species, and has broad application prospects.

Description

technical field [0001] The invention relates to an in vitro cultivation method of plants, in particular to an in vitro cultivation method of the rare plant Aromacarpus japonica. Background technique [0002] Emmenopterys henryi Oliv (Emmenopterys henryi Oliv) is a tall tree of the genus Emmenopterys Oliv in the Rubiaceae family (Rubiaceae). , Morphological evolution and important materials of China's phytogeographical flora. The material of the fragrant fruit tree is white and dense, with straight texture and rapid growth. The bark fiber is the raw material for making wax paper and artificial cotton. It is an excellent material and tree species for afforestation; Nice landscaping and garden ornamental plants. The plant not only has important scientific value in the study of plant evolution, but also has high economic value. However, this species is mostly scattered and the population is small. It has been listed as a national second-class key protected plant and a nationa...

AI Technical Summary

Problems solved by technology

However, there are still few studies on the mass propagation of Xiangguo tree through somatic embryogenesis. Only Ji Feiteng et al. use the leaves of Xiangguo tree as explants to induce somatic embryos (Ji Feiteng, Li Fenglan, Gao Shumin, Chen Faju. Xiangguo somatic cell Embryogenesis [J]. Plant Physiology Communications, 2005, 41, 619-621.), but there is still the problem of low reproductive coefficient

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