Pig fat deposition related protein and encoding genes and use thereof

A technology of fat deposition and pig fat, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of delaying the progress of breeding, cloning without reaching the position, and limited number of detected genes

Inactive Publication Date: 2010-02-24
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

[0005] Although some important progress has been made in the research of candidate genes affecting fat deposition performance in pigs, there are still deficiencies: (1) The important economic traits of pigs are usually quantitative traits, and the physiological and biochemical processes involved are quite complicated. , although 1-2 of its controlled genes have been revealed, there are still other novel genes with large effects to be discovered
(2) Whole-genome scanning using markers can only reflect the regional distribution of some genes that affect traits, and cannot find out all QTLs that affect a certain quantity of traits, and the use of QTLs for positional cloning has not yet been achieved to obtain the purpose genetic requirements
(3) The current research on quantitative trait genes basically selects a single candidate gene for analysis, ignoring the interaction between genes
One of the contents of modern molecular breeding is genome breeding, which is based on the overall or whole genome selection, mating, species conservation and heterosis analysis and utilization based on the organizational structure and functional effects of the genes and genotypes of all traits of the individual. A complete high-density gene map, and a full understanding of the organizational structure, function, expression regulation mechanism, and association with traits of all genes. However, the genes and markers that have been genetically and physically mapped in pigs are still very limited. Work still needs to be further strengthened
(4) The method for finding genes with important physiological functions is not comprehensive enough, the number of detected genes is limited, the efficiency is not high, and innovation is needed, and further work to find new genes related to important economic traits of pigs is imminent
In addition, the carcass traits of pigs, except the backfat thickness, can be directly measured on the live body, and other indicators such as: slaughter rate, leg butt meat and bone ratio, internal fat ratio, eye muscle area, etc. need to be measured after slaughter, thus delaying the breeding progress

Method used

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  • Pig fat deposition related protein and encoding genes and use thereof
  • Pig fat deposition related protein and encoding genes and use thereof
  • Pig fat deposition related protein and encoding genes and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, the acquisition of pig fat deposition-related protein (CGI-146) and its full-length cDNA gene and genomic gene (pig PNAS-4 gene)

[0053] 1. Acquisition of full-length cDNA gene of porcine fat deposition-related protein

[0054] Total RNA was extracted from the skeletal muscle of the 55-day-old large white pig embryo. After mRNA purification, cDNA synthesis, proteinase K and SfiI digestion, and cDNA fractionation, the obtained cDNA was combined with pBluescript II SK + Vector ligation, thereby constructing pig fetal skeletal muscle cDNA library (Zhu Zhengmao, doctoral dissertation, Huazhong Agricultural University, 2003). Clones were randomly selected for commercial sequencing, and the results obtained by the sequencing were non-redundant by the National Center for Biotechnology Information (NCBI, National Center for Biotechnology Information, http: / / www.ncbi.nlm.nih.gov / BLAST / ) Retrieval database (nr) and obtain the corresponding sequence biological info...

Embodiment 2

[0068] Embodiment 2, pig PNAS-4 gene physical localization:

[0069] 1. Acquisition of experimental materials for physical positioning

[0070] The pig×rodent somatic cell hybrid panel (SCHP) was used for chromosome region mapping, and the pig radiation hybrid panel (INRA-Minnesota porcine radiation hybrid panel, IMpRH) co-constructed by the University of Minnesota, USA was used for chromosome accuracy. For positioning, see references for the preparation methods of two sets of somatic cell hybrid panels (Yerle et al., A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cytogenet Cell Genet. 1996, 73: 194-202; Yerle et al., Construction of a whole -genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet Cell Genet. 1998, 82: 182-188).

[0071] Among them, SCHP includes 27 somatic hybrid cell lines, Nos. 1-19 are pig×hamster somatic hybrid cell lines, No. 20-27 are pig×mouse somatic hybrid cell lines, and ...

Embodiment 3

[0077] The single nucleotide polymorphism and RFLP polymorphism detection of the partial DNA sequence of embodiment 3, pig PNAS-4

[0078] 1. Detection of single nucleotide polymorphisms in the partial DNA sequence of pig PNAS-4

[0079] 1) Distribution of single nucleotide polymorphisms of pig PNAS-4 gene

[0080] In order to reflect the single nucleotide polymorphism of the porcine PNAS-4 gene more preparedly, two mutation sites with a distance of 20kb were selected for detection in this experiment.

[0081] The DNA samples used for polymorphism detection came from 7 pig herds, see Table 2. Genomic DNA was extracted by conventional methods and stored at -20°C for future use.

[0082] Table 2. Number of populations and samples detected by SNPs

[0083] Variety combination

[0084] Note: LD=Landrace, LW=Larege White, T=Tongcheng

[0085] Using the genome of the material described in Table 2 as a template, the following primers were used to ampl...

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Abstract

The present invention discloses a protein relating to pig fat deposition and the coding gene as well as application of the protein. The pig fat deposition related protein is of one of the following amino acid residue sequences: 1) The No 1 sequence in the sequence table; 2) the No 1 amino acid residue sequence with one or more than one amino acid residue substituted or lost or added; The protein is related to pig fat deposition. The protein relating to pig fat deposition and the correspondent coding gene can be used to detect the body traits such as suet rate, inner-lipid rate, the back-fat thickness between the 6 to 7 inter-ribs and the average back-fat thickness of the pig that can reflect the fat deposition capability. Therefore, the present invention provided a new genetic marker for the pig molecule breeding, and plays an important role in the pig breeding.

Description

technical field [0001] The present invention relates to a pig fat deposition-related protein and its coding gene and application, in particular to a pig fat deposition-related protein and its coding gene and a method for detecting pig fat deposition performance by using the single nucleotide polymorphism of the protein coding gene . Background technique [0002] Modern breeding technology has significantly improved the production performance of pigs. However, with the continuous improvement of people's living standards, pork consumption has changed from the previous fat type to the current lean type. Correspondingly, the production of large-scale commercial pigs has also changed from quantity to quality. In today's pork market, hot carcass weight and lean yield determine the commercial value of a pig carcass. The grading standards of the world's pig-raising developed countries also divide pig carcasses into different grades according to the difference in lean percentage in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12C12Q1/68
Inventor 李奎莫德林朱正茂唐中林李新云王恒杨述林
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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