The pIFN-gamma gene adjuvant for pig vaccine and its prepn process
A gene adjuvant and adjuvant technology, applied in the field of vaccines for pigs, can solve the problems of swelling, pain, fever, allergies, adverse reactions and the like, and achieve the effects of simple preparation, reduced preparation cost and low cost
Active Publication Date: 2010-03-24
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology
It acts as a storage and carrier for antigens, slowly releasing antigens to prolong the induction of the humoral immune response of the body, but the disadvantage is that it only induces Th2 humoral immune response, and the antibodies are mainly IgG1 type without TCL reaction
Although oil adjuvant and Freund's complete adjuvant can significantly improve the immune response, adverse reactions often occur in practical applications, such as swelling at the injection site, pain, fever, or allergies, etc., and are limited to laboratory animal experiments
206 adjuvant is a high-efficiency animal vaccine oil adjuvant that is currently commercialized. It has been widely used in animal vaccines. Practice has proved that it is reliable, effective and safe, but it needs to be imported and the price is high
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preparation example Construction
[0022] The preparation method of the adjuvant of the present invention is detailed as follows:
[0023] (1) Isolate mononuclear cells from the peripheral blood or lymph nodes of pigs, culture them with inducers, and clone the target cytokine genes by RT-PCR. After sequence homology, genetic evolution, and their molecular structure and function prediction analysis , As a candidate gene for gene adjuvant;
[0024] (2) Using genetic engineering recombination technology, select appropriate expression primers containing restriction sites to clone the target cytokine gene into an animal cell expression plasmid vector;
[0025] (3) Transform the prokaryotic host bacteria with the recombinant plasmid vector obtained in step (2), select positive clones, and then carry out amplification culture after sequencing and identification; and
[0026] (4) Mass extraction and purification of recombinant plasmid DNA of the target gene.
[0027] The plasmid vector in step (2) is a vector commonly used in g...
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Abstract
The present invention discloses one kind of adjuvant for pig vaccine and its preparation process and constituted pig vaccine. The gene adjuvant for pig vaccine is adjuvant including pig gamma-interferon gene (pIFN-gamma), or recombinant plasmid pcDNA-pIFN-gamma of animal cell expression plasmid pcDNA3.1 and pig gamma-interferon gene (pIFN-gamma), or the combination of pcDNA-pIFN-gamma and No. 206adjuvant. The pig vaccine is composition of pig's cysticercus-resisting vaccine composition; pig's deactivated foot-and-mouth disease virus vaccine and the gene adjuvant. The gene adjuvant of the present invention is prepared through gene cloning, recombination, recombinant plasmid proliferation, extraction and purification and other processes.
Description
Technical field [0001] The invention relates to an adjuvant used in a pig vaccine and a preparation method thereof, and a pig vaccine composed of the adjuvant of the invention. Background technique [0002] Since the 1920s, many substances have been tried as immune adjuvants, but only aluminum gel adjuvants have been licensed for human vaccines and have been widely used in pig vaccines. It serves as a storage and carrier for antigens, slowly releasing antigens to prolong the body's humoral immune response, but the disadvantage is that it only induces Th2 humoral immune response. The antibody is mainly of IgG1 type without TCL response. Although oil adjuvant and Freund's complete adjuvant can significantly improve the immune response, adverse reactions such as swelling, pain, fever, or allergies at the injection site often occur in practical applications. They are only used in laboratory animal experiments. 206 adjuvant is currently commercialized high-efficiency animal vaccine o...
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IPC IPC(8): A61K39/39A61K39/002A61K39/135A61K9/107A61K48/00A61P31/12A61P33/02C12N15/09
Inventor 景志忠才学鹏窦永喜蒙学莲王佩雅陈国华罗启慧袁改玲侯俊玲骆学农
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI