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Micromai's interference biomolecule action sensing method and probe

A technology of biomolecules and sensing methods, which is applied in the field of micro-Myers interference biomolecular action sensing methods and probes, can solve the problems of large optical path and circuit interference, poor vibration resistance, large temperature control range, etc., and achieve stray light. Small interference with the optical path, both versatility and selectivity, and the effect of small constant temperature and temperature control interval

Inactive Publication Date: 2007-07-18
重庆工学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The deficiencies of the above-mentioned methods are: (a) the sensing of biomolecular action and its temperature is separated, and different sensing methods are used in different spaces to realize the sensing of temperature and biomolecular action; When the biomolecules are sensitive to the film, the temperature of the biomolecular action point is different from the measured value, and the small amount of the difference will seriously affect the temperature correction, and sometimes even make the sensing of the biomolecular action difficult to realize; (b) Sensors that implement these methods The structure is complex, basically composed of separate components, with large volume, poor vibration resistance, high cost, difficult remote measurement, and large temperature control range; (c) the sensing system that implements these methods has no data fault tolerance, and the optical path and circuit interfere greatly. demanding working conditions

Method used

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  • Micromai's interference biomolecule action sensing method and probe
  • Micromai's interference biomolecule action sensing method and probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Referring to Fig. 2, the structure of the micro-Mei's interference biomolecular action probe includes: optical fiber cladding 1, optical fiber core 2, long-period fiber grating 3, short-period fiber grating 4, biomolecular sensitive film 6, quartz Support frame 7, reflector 8, first curing adhesive 9, second curing adhesive 10, third curing adhesive 11, leak-proof gasket 12, guide sleeve 13, threaded cap 14, limit ring 15, optical fiber transition buffer sleeve 16, The fourth curing glue 17 and the optical fiber protective layer 18 . Its composition method is as follows: (a) On a section of optical fiber with optical fiber core 2, optical fiber cladding 1, and optical fiber protective layer 18, remove part of the optical fiber protective layer, and use a laser and a long-period fiber grating mask to write a 20mm long, 3dB bandwidth 10nm, long-period fiber grating 3 with a co-coupling efficiency of 4dB at the center wavelength of the co-coupling; the long-p...

Embodiment 2

[0030] Embodiment 2: Referring to Fig. 3, the difference between the structure of the micro-Mei's interference biomolecular action probe and the probe structure of embodiment 1 is that there is a metal film 5 between the optical fiber cladding 1 and the biomolecular sensitive film 6, The material of the metal film 5 is gold, and the thickness of the metal film 5 is 30 nm, and the other is the same as the probe in the first embodiment.

[0031] Using the probe of the second embodiment, the difference between the corresponding embodiment of the sensing method and the sensing method of the first embodiment is that the light in the optical fiber cladding 1 is totally reflected at the interface between the optical fiber cladding 1 and the metal film 5 When surface plasmon resonance (SPR) will be generated, it will be attenuated and cause a phase change. The central wavelength of SPR attenuation, the attenuation amplitude at a specific wavelength and its phase change are affected by ...

Embodiment 3

[0032] Embodiment three: Referring to Fig. 4, the difference between the structure of the micro-Maysonian interference biomolecular action probe and the probe structure of embodiment one is: use the superstructure fiber grating 19 as the forward and reverse coupler to replace the long-period fiber grating 3 and short-period fiber gratings 4. The forming method of this superstructure fiber grating 19 is: use the method for overlapping and moving the long-period fiber grating template and the short-period fiber grating template and the laser to make the superstructure fiber grating 19 on the optical fiber, so that the same direction of the superstructure fiber grating 19 The coupling center wavelength is around 1540nm, the coupling efficiency at the center wavelength of the co-coupling is 3dB, the wavelength bandwidth of the 3dB co-coupling is 10nm, the center wavelength of the reverse coupling is around 1550nm, and the reverse coupling at the center wavelength of the reverse cou...

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Abstract

The invention discloses micro Myers interference organism molecule action sensing method and probe. The method includes the following steps: using micro Michelson interferometer and organism molecule sensing film to gain organism molecule action information, temperature information by coupler to realize organism molecule action sensing after rectifying temperature precision, and gain back scattered light or fluorescence distributing information. It has data fault tolerant ability, universal and selectivity, same time and space sensing temperature information, little noise, interference, and stray light, high sensitivity, and big measuring range. The probe is made up of positive and negative coupler, reflector, organism molecule sensing film, has the advantages of integration, miniaturization, little temperature control area, good anti vibration, low cost etc. The method and probe can be used in organism molecule action or its heat dynamic behavior sensing.

Description

technical field [0001] The invention belongs to the fields of biotechnology, analytical instruments and sensing technology, and in particular relates to a micro-Maysonian interference biomolecular action sensing method and a probe. Background technique [0002] Biomolecular interactions are the basis of life, and many biological studies can be attributed to the study of biomolecular interactions. The interaction of biomolecules is closely related to temperature and is greatly affected by temperature. In order to obtain more biomolecular information, people need to obtain the thermodynamic properties of biomolecular interactions. In the existing biomolecular interaction sensing methods, the signal changes generated by the biomolecular interaction are extremely weak, and the sensing signal itself is greatly affected by temperature changes, and the signal changes caused by extremely small temperature fluctuations are enough to overwhelm the biomolecular interaction information...

Claims

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Application Information

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IPC IPC(8): G01N21/45G01N33/53G06F19/00
Inventor 曾祥楷
Owner 重庆工学院